Journal: Antioxidants & Redox Signaling

Article Title: The Cysteine Dioxgenase Knockout Mouse: Altered Cysteine Metabolism in Nonhepatic Tissues Leads to Excess H 2 S/HS − Production and Evidence of Pancreatic and Lung Toxicity

doi: 10.1089/ars.2012.5010

Figure Lengend Snippet: Tissue and serum taurine and hypotaurine levels and abundance of CDO, CSD, ADO, and SLC6A6 in female and male null mice and female and male wild-type mice fed a diet enriched in sulfur amino acids without or with supplemental 1% (w/w) taurine. (A, B) Taurine and hypotaurine levels. Values are means±SEM for seven mice. Values for a given tissue that are not denoted with the same letter are significantly different (p<0.05) by Tukey's test. Numerical values and details of the statistical analysis are given in Supplementary Table S1. (C) Western blots for CDO, CSD, ADO, and SLC6A6. Blots are for pooled samples (equal amounts of protein from each of seven mice in the group). Efforts to measure pancreatic CSD and SLC6A6 levels were not successful due to nonspecific antibody reactions. Western blots were quantified and normalized by actin, and the data were analyzed by a general linear model for the three categorical variables: genotype (N, null; W, wild-type); taurine supplementation (−, no taurine; +, with taurine); sex (F, female; M, male); and some two-way interactions. Differences significant at p<0.05 are indicated as the direction of difference. Relative intensities of the bands are reported in Supplementary Table S2. ADO, 2-aminoethanethioldioxygenase; CSD, cysteine sulfinate decarboxylase; SLC6A6, solute carrier 6A6 (taurine transporter).

Article Snippet: Other primary antibodies were obtained from commercial sources: COX5b and COX4 (Abcam), CBS, SQRDL, SUOX and TST (Proteintech Group), CTH (Norvus Biologicals), MPST (Santa Cruz Biotechnology), actin (Cell Signaling), ETHE1 (Thermo Scientific), and SLC6A6 (TAU11-A; Alpha Diagnostic International).

Techniques: Western Blot

Journal: ACS Omega

Article Title: DataPype: A Fully Automated Unified Software Platform for Computer-Aided Drug Design

doi: 10.1021/acsomega.3c05207

Figure Lengend Snippet: Benchmarking and Optimization of the Ligand Preparation Methodology Based on Benchmarking Metrics (AUC and EF1%) Calculation and Time (Minutes) Required to Complete Ligand Preparation and Docking (Using FRED) a

Article Snippet: (iii) taut_maxgenerat_4096 (default value of openEye) , 0.72 , 18 , 9.7 , 5.1.

Techniques: