tar-nm-u23 Search Results


tar-nm-u23  (BioAutomation corporation)
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BioAutomation corporation tar-nm-u23
a Overlay of conformers showing motions in bulge residues for linear (| β h | < 45°), intermediate bend (45° < | β h | < 70°) and kinked (| β h | > 70°) inter-helical conformations in the FARFAR-NMR and Anton-MD-NMR ensembles. b , c The fractional populations of conformers with the number of b extra-helical and c C2′- endo residues (color-coded) in the bulge residues <t>(U23,</t> C24, and U25) as a function of bending angle in the FARFAR-NMR (“F”) and Anton-MD-NMR (“A”) ensembles ( N = 20). d Comparison of one coaxially stacked FARFAR-NMR conformer with the crystal structure of Ca 2+ -bound <t>TAR</t> (PDBID: 397D) . e Nm shifts sugar-pucker equilibrium towards C3′- endo . f Overlay of 2D [ 13 C, 1 H] HSQC NMR spectra of the aromatic spins for TAR-Nm-C24 without Mg 2+ (blue) with blue arrows indicate unstacking, TAR without Mg 2+ (cyan) and TAR with 3 mM Mg 2+ (red) with red arrows indicating increased coaxial stacking.
Tar Nm U23, supplied by BioAutomation corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tar-nm-u23/pmc07608651-253-1-14?v=BioAutomation+corporation
Average 90 stars, based on 1 article reviews
tar-nm-u23 - by Bioz Stars, 2026-08
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a Overlay of conformers showing motions in bulge residues for linear (| β h | < 45°), intermediate bend (45° < | β h | < 70°) and kinked (| β h | > 70°) inter-helical conformations in the FARFAR-NMR and Anton-MD-NMR ensembles. b , c The fractional populations of conformers with the number of b extra-helical and c C2′- endo residues (color-coded) in the bulge residues (U23, C24, and U25) as a function of bending angle in the FARFAR-NMR (“F”) and Anton-MD-NMR (“A”) ensembles ( N = 20). d Comparison of one coaxially stacked FARFAR-NMR conformer with the crystal structure of Ca 2+ -bound TAR (PDBID: 397D) . e Nm shifts sugar-pucker equilibrium towards C3′- endo . f Overlay of 2D [ 13 C, 1 H] HSQC NMR spectra of the aromatic spins for TAR-Nm-C24 without Mg 2+ (blue) with blue arrows indicate unstacking, TAR without Mg 2+ (cyan) and TAR with 3 mM Mg 2+ (red) with red arrows indicating increased coaxial stacking.

Journal: Nature Communications

Article Title: Rapid and accurate determination of atomistic RNA dynamic ensemble models using NMR and structure prediction

doi: 10.1038/s41467-020-19371-y

Figure Lengend Snippet: a Overlay of conformers showing motions in bulge residues for linear (| β h | < 45°), intermediate bend (45° < | β h | < 70°) and kinked (| β h | > 70°) inter-helical conformations in the FARFAR-NMR and Anton-MD-NMR ensembles. b , c The fractional populations of conformers with the number of b extra-helical and c C2′- endo residues (color-coded) in the bulge residues (U23, C24, and U25) as a function of bending angle in the FARFAR-NMR (“F”) and Anton-MD-NMR (“A”) ensembles ( N = 20). d Comparison of one coaxially stacked FARFAR-NMR conformer with the crystal structure of Ca 2+ -bound TAR (PDBID: 397D) . e Nm shifts sugar-pucker equilibrium towards C3′- endo . f Overlay of 2D [ 13 C, 1 H] HSQC NMR spectra of the aromatic spins for TAR-Nm-C24 without Mg 2+ (blue) with blue arrows indicate unstacking, TAR without Mg 2+ (cyan) and TAR with 3 mM Mg 2+ (red) with red arrows indicating increased coaxial stacking.

Article Snippet: TAR, TAR-Nm-U23, and TAR-Nm-C24 RNA samples were synthesized using a MerMade 6 Oligo Synthesizer (BioAutomation) via solid-phase synthesis using standard phosphoramidite chemistry and deprotection protocols.

Techniques: