Journal: European Respiratory Review

Article Title: The microbiome in bronchiectasis

doi: 10.1183/16000617.0048-2019

Figure Lengend Snippet: Summary of bronchiectasis microbiome studies

Article Snippet: T aylor (BLESS) [ ] , 2015 , 86 , Sputum , Patients required to be clinically stable, not receiving systemic corticosteroids and macrolide naive , Laboratory-specific protocol including a bead-beating step and a phenol/chloroform precipitation , V1-V3 , Bacterial tag- endocoded FLX amplicon pyrosequencing , Custom C# software in MicrosoftH.NET environment , Patients stratified as Haemophilus- or Pseudomonas- or other-dominated microbiomes.

Techniques: DNA Extraction, Sequencing, Transplantation Assay, Software, Amplification

Journal: International Journal of Molecular Sciences

Article Title: Human Male Genital Tract Microbiota

doi: 10.3390/ijms24086939

Figure Lengend Snippet: Characterisation of penile microbial communities using NGS.

Article Snippet: Nelson et al., 2012 [ ] , Pyrosequencing (V1–V3, V3–V5 and V6–V9) , 18 , , No , Characterisation of coronal sulcus (CS) and urine microbiota in adolescent men. The three most abundant genera were Corynebacterium , Staphylococcus , and Anaerococcus , representing more than 58.9% of total sequences, followed by Peptoniphilus , Prevotella , Finegoldia , Porphyromonas , Propionibacterium, and Delftia . CS microbiota was stable during study interval, especially for Staphyloccoccus , Mobiluncus , Prevotella , Dialister, and Anaerococcus genera. Distinct bacterial communities between CS and urine samples and between circumcised and noncircumcised men..

Techniques: Sequencing, Comparison, Bacteria, Virus

Journal: International Journal of Molecular Sciences

Article Title: Human Male Genital Tract Microbiota

doi: 10.3390/ijms24086939

Figure Lengend Snippet: Characterisation of seminal microbiota using NGS.

Article Snippet: Nelson et al., 2012 [ ] , Pyrosequencing (V1–V3, V3–V5 and V6–V9) , 18 , , No , Characterisation of coronal sulcus (CS) and urine microbiota in adolescent men. The three most abundant genera were Corynebacterium , Staphylococcus , and Anaerococcus , representing more than 58.9% of total sequences, followed by Peptoniphilus , Prevotella , Finegoldia , Porphyromonas , Propionibacterium, and Delftia . CS microbiota was stable during study interval, especially for Staphyloccoccus , Mobiluncus , Prevotella , Dialister, and Anaerococcus genera. Distinct bacterial communities between CS and urine samples and between circumcised and noncircumcised men..

Techniques: Sequencing, Bacteria, Infection, Concentration Assay, Virus, Isolation

Journal: Frontiers in Immunology

Article Title: Analyzing Immunoglobulin Repertoires

doi: 10.3389/fimmu.2018.00462

Figure Lengend Snippet: Common platforms used for immunoglobulin repertoire sequencing.

Article Snippet: Mechanism , Pyrosequencing , Dye terminator sequencing , Syntdesis (fluoresces attached to nt is excited and detected after each run) , Syntdesis (florescence tag attached to phosphate chain) , Syntdesis (detect H + ).

Techniques: Sequencing, Amplification