Journal: Proceedings

Article Title: Autocrine Growth Hormone Mediated Curcumin Resistance Overcame by Autophagy Inhibition via Bafilomycin in MDA-MB-231 and T47D Breast Cancer Cells

doi: 10.3390/proceedings2251569

Figure Lengend Snippet: Figure 1. Curcumin induces cell viability loss in time dependent manner. (a) MDA-MB-231 wt and GH+; (b) T47D wt and GH+ breast cancer cells were seeded in 6 well-plate and curcumin (25 µΜ for MDA-MB-231 and 20 µM for T47D cells) exposed to cells in time-dependent (0–24 h) manner. Following drug exposure cells were stained by Mitotracker for 15 min and DAPI staining for 5 min, respectively and Mitotracker/DAPI stained cells were visualized by fluorescence microscopy.

Article Snippet: Time-dependent curcumin (Sigma Aldrich, C1386, Munich, Germany) effect on cell growth and proliferation was determined by Mitotracker/DAPI (Thermo fisher Scientific, Mitotracker Red CMXRos, M7512, DAPI, D1306, Waltham, MA, USA) staining in T47D (ATCC, HTB-133, Manassas, VA, USA) and MDA-MB-231 (ATCC, HTB-26, Manassas, VA, USA) breast cancer cells.

Techniques: Staining, Fluorescence, Microscopy

Journal: Proceedings

Article Title: Autocrine Growth Hormone Mediated Curcumin Resistance Overcame by Autophagy Inhibition via Bafilomycin in MDA-MB-231 and T47D Breast Cancer Cells

doi: 10.3390/proceedings2251569

Figure Lengend Snippet: Figure 2. Time-dependent curcumin induced autophagy and ER stress in MDA-MB-231 and T47D wt/GH+ breast cancer cells. The time dependent effect of curcumin on autophagy key players (Beclin- 1, Atg3, Atg12, LC3) and ER stress (BiP, IRE1α and Calreticulin) molecules expression profile was determined by immunoblotting.

Article Snippet: Time-dependent curcumin (Sigma Aldrich, C1386, Munich, Germany) effect on cell growth and proliferation was determined by Mitotracker/DAPI (Thermo fisher Scientific, Mitotracker Red CMXRos, M7512, DAPI, D1306, Waltham, MA, USA) staining in T47D (ATCC, HTB-133, Manassas, VA, USA) and MDA-MB-231 (ATCC, HTB-26, Manassas, VA, USA) breast cancer cells.

Techniques: Expressing, Western Blot

Journal: Proceedings

Article Title: Autocrine Growth Hormone Mediated Curcumin Resistance Overcame by Autophagy Inhibition via Bafilomycin in MDA-MB-231 and T47D Breast Cancer Cells

doi: 10.3390/proceedings2251569

Figure Lengend Snippet: Figure 3. Effect of bafilomycin on curcumin-induced cell viability loss. Determined dose of curcumin (25 µΜ for MDA-MB-231 and 20 µM for T47D cells) for 24 h treatment with/without Bafilomycin (50 nM) in (a) MDA-MB-231 wild type and GH+ and (b) T47D wild type and GH+ and MTT cell viability assay was performed. The columns represent mean ± S.D. of two independent experiments and at least 3 replicates. B: Bafilomycin, C: Curcumin, B + C: Bafilomycin and Curcumin.

Article Snippet: Time-dependent curcumin (Sigma Aldrich, C1386, Munich, Germany) effect on cell growth and proliferation was determined by Mitotracker/DAPI (Thermo fisher Scientific, Mitotracker Red CMXRos, M7512, DAPI, D1306, Waltham, MA, USA) staining in T47D (ATCC, HTB-133, Manassas, VA, USA) and MDA-MB-231 (ATCC, HTB-26, Manassas, VA, USA) breast cancer cells.

Techniques: Viability Assay

Journal: Frontiers in Oncology

Article Title: Carnosol, a Natural Polyphenol, Inhibits Migration, Metastasis, and Tumor Growth of Breast Cancer via a ROS-Dependent Proteasome Degradation of STAT3

doi: 10.3389/fonc.2019.00743

Figure Lengend Snippet: Carnosol inhibits the STAT3 signaling pathway. (A) Concentration-dependent decrease of phospho-STAT3 and STAT3 protein in carnosol-treated MDA-MB-231 cells. Cells were treated with vehicle (DMSO) or indicated concentrations of carnosol for 24 h, then whole-cell extracts were subjected to Western blot analysis for the phosphorylated and non-phosphorylated form of STAT3 and for β-actin (loading control). (B) Carnosol decreases the level of STAT3 protein in Hs578T, MCF-7, and T47D breast cancer cell lines. Whole cell lysates from different breast cancer cell lines treated with the indicated concentrations of carnosol were resolved on 8% SDS-PAGE and analyzed by western blot for STAT3 protein. (C) Western blot analysis of STAT3 protein level in HCT116 colorectal cancer cells. Western blotting was performed as described above.

Article Snippet: Human breast cancer cells MDA-MB-231(cat# 300275), MCF-7 (cat# 300273) and T47D (cat# 300353) were purchased from Cell Line Service (CLS)-GmbH.

Techniques: Concentration Assay, Western Blot, Control, SDS Page

Journal: PLOS One

Article Title: Ivermectin inhibits ER, HER2, and TGF-β pathways in ER-positive and endocrine-resistant breast cancer cells

doi: 10.1371/journal.pone.0348260

Figure Lengend Snippet: MTT assay measured cell viability of ER-positive breast cancer cells: (A) MCF-7 and (B) T-47D, were treated with (+E 2 ) or without (−E 2 ) 17β-estradiol at physiologic level (10 nM E 2 ) in the presence of nontoxic concentrations of IVM (1, 3, 5 μM) or 4-OHT (5, 10 μM) as a positive control for 5 days. (C–E) The inhibitory effect of IVM on ERα and HER2 protein levels of ER-positive breast cancer was performed after the treatments with various concentrations of IVM with (+E 2 ) or without (−E 2 ) 17β-estradiol (10 nM E 2 ) for 24 h in MCF-7. (F) mRNA expression of the ESR1 gene (ERα) was determined by qPCR analysis in MCF-7 cells. The graphs showed the mean ± SEM. The blue and red asterisks highlight the significant differences in comparison to non-treatment without (−E 2 ) and with (+E 2 ), respectively. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 versus the non-treatment control (n = 3).

Article Snippet: The ER-positive breast cancer cell lines: MCF-7 and T-47D, along with the tamoxifen-resistant T-47D Tam1, were obtained from the American Type Culture Collection: ATCC (Virginia, USA).

Techniques: MTT Assay, Positive Control, Expressing, Comparison, Control

Journal: Frontiers in Veterinary Science

Article Title: Characterization of Nuclear Progesterone Receptor Isoforms in the Term Equine Placenta

doi: 10.3389/fvets.2021.660177

Figure Lengend Snippet: Progesterone receptor protein expression in equine placental tissue at term ( n = 6). Immunoblot data show PRA and PRB, as well as β-actin as a loading control band. The T47D lysate was used as a positive control for PRA and PRB bands.

Article Snippet: T47D whole cell lysate (Santa Cruz Biotechnology, Santa Cruz, CA, USA, Cat# SC-2293) was used as the positive control for PRA and PRB in Western Blot analysis.

Techniques: Expressing, Western Blot, Control, Positive Control