t 47d Search Results


t47d  (ATCC)
99
ATCC t47d
T47d, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human breast cancer cell lines  (ATCC)
97
ATCC human breast cancer cell lines
Human Breast Cancer Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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t47d  (DSMZ)
95
DSMZ t47d
T47d, supplied by DSMZ, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tamoxifen resistant t 47d tam1  (ATCC)
94
ATCC tamoxifen resistant t 47d tam1
MTT assay measured cell viability of ER-positive breast cancer cells: (A) MCF-7 and (B) <t>T-47D,</t> were treated with (+E 2 ) or without (−E 2 ) 17β-estradiol at physiologic level (10 nM E 2 ) in the presence of nontoxic concentrations of IVM (1, 3, 5 μM) or 4-OHT (5, 10 μM) as a positive control for 5 days. (C–E) The inhibitory effect of IVM on ERα and HER2 protein levels of ER-positive breast cancer was performed after the treatments with various concentrations of IVM with (+E 2 ) or without (−E 2 ) 17β-estradiol (10 nM E 2 ) for 24 h in MCF-7. (F) mRNA expression of the ESR1 gene (ERα) was determined by qPCR analysis in MCF-7 cells. The graphs showed the mean ± SEM. The blue and red asterisks highlight the significant differences in comparison to non-treatment without (−E 2 ) and with (+E 2 ), respectively. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 versus the non-treatment control (n = 3).
Tamoxifen Resistant T 47d Tam1, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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collection human endocarditis hdp 98354 cdc 3437 70 human  (ATCC)
90
ATCC collection human endocarditis hdp 98354 cdc 3437 70 human
MTT assay measured cell viability of ER-positive breast cancer cells: (A) MCF-7 and (B) <t>T-47D,</t> were treated with (+E 2 ) or without (−E 2 ) 17β-estradiol at physiologic level (10 nM E 2 ) in the presence of nontoxic concentrations of IVM (1, 3, 5 μM) or 4-OHT (5, 10 μM) as a positive control for 5 days. (C–E) The inhibitory effect of IVM on ERα and HER2 protein levels of ER-positive breast cancer was performed after the treatments with various concentrations of IVM with (+E 2 ) or without (−E 2 ) 17β-estradiol (10 nM E 2 ) for 24 h in MCF-7. (F) mRNA expression of the ESR1 gene (ERα) was determined by qPCR analysis in MCF-7 cells. The graphs showed the mean ± SEM. The blue and red asterisks highlight the significant differences in comparison to non-treatment without (−E 2 ) and with (+E 2 ), respectively. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 versus the non-treatment control (n = 3).
Collection Human Endocarditis Hdp 98354 Cdc 3437 70 Human, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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goat polyclonal anti claudin 1  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology goat polyclonal anti claudin 1
MTT assay measured cell viability of ER-positive breast cancer cells: (A) MCF-7 and (B) <t>T-47D,</t> were treated with (+E 2 ) or without (−E 2 ) 17β-estradiol at physiologic level (10 nM E 2 ) in the presence of nontoxic concentrations of IVM (1, 3, 5 μM) or 4-OHT (5, 10 μM) as a positive control for 5 days. (C–E) The inhibitory effect of IVM on ERα and HER2 protein levels of ER-positive breast cancer was performed after the treatments with various concentrations of IVM with (+E 2 ) or without (−E 2 ) 17β-estradiol (10 nM E 2 ) for 24 h in MCF-7. (F) mRNA expression of the ESR1 gene (ERα) was determined by qPCR analysis in MCF-7 cells. The graphs showed the mean ± SEM. The blue and red asterisks highlight the significant differences in comparison to non-treatment without (−E 2 ) and with (+E 2 ), respectively. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 versus the non-treatment control (n = 3).
Goat Polyclonal Anti Claudin 1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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t47d cells  (CLS Cell Lines Service GmbH)
90
CLS Cell Lines Service GmbH t47d cells
MTT assay measured cell viability of ER-positive breast cancer cells: (A) MCF-7 and (B) <t>T-47D,</t> were treated with (+E 2 ) or without (−E 2 ) 17β-estradiol at physiologic level (10 nM E 2 ) in the presence of nontoxic concentrations of IVM (1, 3, 5 μM) or 4-OHT (5, 10 μM) as a positive control for 5 days. (C–E) The inhibitory effect of IVM on ERα and HER2 protein levels of ER-positive breast cancer was performed after the treatments with various concentrations of IVM with (+E 2 ) or without (−E 2 ) 17β-estradiol (10 nM E 2 ) for 24 h in MCF-7. (F) mRNA expression of the ESR1 gene (ERα) was determined by qPCR analysis in MCF-7 cells. The graphs showed the mean ± SEM. The blue and red asterisks highlight the significant differences in comparison to non-treatment without (−E 2 ) and with (+E 2 ), respectively. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 versus the non-treatment control (n = 3).
T47d Cells, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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evs isolated from t-47d to-nfat3  (NanoSight ltd)
90
NanoSight ltd evs isolated from t-47d to-nfat3
MTT assay measured cell viability of ER-positive breast cancer cells: (A) MCF-7 and (B) <t>T-47D,</t> were treated with (+E 2 ) or without (−E 2 ) 17β-estradiol at physiologic level (10 nM E 2 ) in the presence of nontoxic concentrations of IVM (1, 3, 5 μM) or 4-OHT (5, 10 μM) as a positive control for 5 days. (C–E) The inhibitory effect of IVM on ERα and HER2 protein levels of ER-positive breast cancer was performed after the treatments with various concentrations of IVM with (+E 2 ) or without (−E 2 ) 17β-estradiol (10 nM E 2 ) for 24 h in MCF-7. (F) mRNA expression of the ESR1 gene (ERα) was determined by qPCR analysis in MCF-7 cells. The graphs showed the mean ± SEM. The blue and red asterisks highlight the significant differences in comparison to non-treatment without (−E 2 ) and with (+E 2 ), respectively. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 versus the non-treatment control (n = 3).
Evs Isolated From T 47d To Nfat3, supplied by NanoSight ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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breast cancer cell line t-47d  (CEM Corporation)
90
CEM Corporation breast cancer cell line t-47d
MTT assay measured cell viability of ER-positive breast cancer cells: (A) MCF-7 and (B) <t>T-47D,</t> were treated with (+E 2 ) or without (−E 2 ) 17β-estradiol at physiologic level (10 nM E 2 ) in the presence of nontoxic concentrations of IVM (1, 3, 5 μM) or 4-OHT (5, 10 μM) as a positive control for 5 days. (C–E) The inhibitory effect of IVM on ERα and HER2 protein levels of ER-positive breast cancer was performed after the treatments with various concentrations of IVM with (+E 2 ) or without (−E 2 ) 17β-estradiol (10 nM E 2 ) for 24 h in MCF-7. (F) mRNA expression of the ESR1 gene (ERα) was determined by qPCR analysis in MCF-7 cells. The graphs showed the mean ± SEM. The blue and red asterisks highlight the significant differences in comparison to non-treatment without (−E 2 ) and with (+E 2 ), respectively. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 versus the non-treatment control (n = 3).
Breast Cancer Cell Line T 47d, supplied by CEM Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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t-47d  (Walser GmbH)
90
Walser GmbH t-47d
MTT assay measured cell viability of ER-positive breast cancer cells: (A) MCF-7 and (B) <t>T-47D,</t> were treated with (+E 2 ) or without (−E 2 ) 17β-estradiol at physiologic level (10 nM E 2 ) in the presence of nontoxic concentrations of IVM (1, 3, 5 μM) or 4-OHT (5, 10 μM) as a positive control for 5 days. (C–E) The inhibitory effect of IVM on ERα and HER2 protein levels of ER-positive breast cancer was performed after the treatments with various concentrations of IVM with (+E 2 ) or without (−E 2 ) 17β-estradiol (10 nM E 2 ) for 24 h in MCF-7. (F) mRNA expression of the ESR1 gene (ERα) was determined by qPCR analysis in MCF-7 cells. The graphs showed the mean ± SEM. The blue and red asterisks highlight the significant differences in comparison to non-treatment without (−E 2 ) and with (+E 2 ), respectively. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 versus the non-treatment control (n = 3).
T 47d, supplied by Walser GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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t-47d breast cancer cell line–derived xenograft model  (WuXi AppTec)
90
WuXi AppTec t-47d breast cancer cell line–derived xenograft model
MTT assay measured cell viability of ER-positive breast cancer cells: (A) MCF-7 and (B) <t>T-47D,</t> were treated with (+E 2 ) or without (−E 2 ) 17β-estradiol at physiologic level (10 nM E 2 ) in the presence of nontoxic concentrations of IVM (1, 3, 5 μM) or 4-OHT (5, 10 μM) as a positive control for 5 days. (C–E) The inhibitory effect of IVM on ERα and HER2 protein levels of ER-positive breast cancer was performed after the treatments with various concentrations of IVM with (+E 2 ) or without (−E 2 ) 17β-estradiol (10 nM E 2 ) for 24 h in MCF-7. (F) mRNA expression of the ESR1 gene (ERα) was determined by qPCR analysis in MCF-7 cells. The graphs showed the mean ± SEM. The blue and red asterisks highlight the significant differences in comparison to non-treatment without (−E 2 ) and with (+E 2 ), respectively. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 versus the non-treatment control (n = 3).
T 47d Breast Cancer Cell Line–Derived Xenograft Model, supplied by WuXi AppTec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ptripz-psmd2 shrna plasmid  (Broad Institute Inc)
90
Broad Institute Inc ptripz-psmd2 shrna plasmid
MTT assay measured cell viability of ER-positive breast cancer cells: (A) MCF-7 and (B) <t>T-47D,</t> were treated with (+E 2 ) or without (−E 2 ) 17β-estradiol at physiologic level (10 nM E 2 ) in the presence of nontoxic concentrations of IVM (1, 3, 5 μM) or 4-OHT (5, 10 μM) as a positive control for 5 days. (C–E) The inhibitory effect of IVM on ERα and HER2 protein levels of ER-positive breast cancer was performed after the treatments with various concentrations of IVM with (+E 2 ) or without (−E 2 ) 17β-estradiol (10 nM E 2 ) for 24 h in MCF-7. (F) mRNA expression of the ESR1 gene (ERα) was determined by qPCR analysis in MCF-7 cells. The graphs showed the mean ± SEM. The blue and red asterisks highlight the significant differences in comparison to non-treatment without (−E 2 ) and with (+E 2 ), respectively. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 versus the non-treatment control (n = 3).
Ptripz Psmd2 Shrna Plasmid, supplied by Broad Institute Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


MTT assay measured cell viability of ER-positive breast cancer cells: (A) MCF-7 and (B) T-47D, were treated with (+E 2 ) or without (−E 2 ) 17β-estradiol at physiologic level (10 nM E 2 ) in the presence of nontoxic concentrations of IVM (1, 3, 5 μM) or 4-OHT (5, 10 μM) as a positive control for 5 days. (C–E) The inhibitory effect of IVM on ERα and HER2 protein levels of ER-positive breast cancer was performed after the treatments with various concentrations of IVM with (+E 2 ) or without (−E 2 ) 17β-estradiol (10 nM E 2 ) for 24 h in MCF-7. (F) mRNA expression of the ESR1 gene (ERα) was determined by qPCR analysis in MCF-7 cells. The graphs showed the mean ± SEM. The blue and red asterisks highlight the significant differences in comparison to non-treatment without (−E 2 ) and with (+E 2 ), respectively. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 versus the non-treatment control (n = 3).

Journal: PLOS One

Article Title: Ivermectin inhibits ER, HER2, and TGF-β pathways in ER-positive and endocrine-resistant breast cancer cells

doi: 10.1371/journal.pone.0348260

Figure Lengend Snippet: MTT assay measured cell viability of ER-positive breast cancer cells: (A) MCF-7 and (B) T-47D, were treated with (+E 2 ) or without (−E 2 ) 17β-estradiol at physiologic level (10 nM E 2 ) in the presence of nontoxic concentrations of IVM (1, 3, 5 μM) or 4-OHT (5, 10 μM) as a positive control for 5 days. (C–E) The inhibitory effect of IVM on ERα and HER2 protein levels of ER-positive breast cancer was performed after the treatments with various concentrations of IVM with (+E 2 ) or without (−E 2 ) 17β-estradiol (10 nM E 2 ) for 24 h in MCF-7. (F) mRNA expression of the ESR1 gene (ERα) was determined by qPCR analysis in MCF-7 cells. The graphs showed the mean ± SEM. The blue and red asterisks highlight the significant differences in comparison to non-treatment without (−E 2 ) and with (+E 2 ), respectively. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 versus the non-treatment control (n = 3).

Article Snippet: The ER-positive breast cancer cell lines: MCF-7 and T-47D, along with the tamoxifen-resistant T-47D Tam1, were obtained from the American Type Culture Collection: ATCC (Virginia, USA).

Techniques: MTT Assay, Positive Control, Expressing, Comparison, Control