syt 1 Search Results


recombinant human syt1 proteins  (OriGene)
90
OriGene recombinant human syt1 proteins
FIGURE 3 Levels of neuronal markers were not altered in 18-month-old AppNL-F/wt knock-in; ob/ob mice. Levels of NF-H (A), <t>SYT1</t> (B), PSD95 (C), and phospho-tau (D) were assessed using ELISAs and compared among each genotype after adjusting for sex (n = 10-44 mice/group). Standard curve of ELISAs of NF-H (E), SYT1 (F), PSD95 (G), and phospho-tau (H) with sample range as shown in red line in a representative assay. A-D, Data are presented as adjusted means ± standard errors of the means and were compared among each genotype using Tukey's HSD test. APP KI, AppNL-F/wt knock-in; APP KI ob/ob, AppNL-F/wt knock-in; ob/ob mice; NS, not significant; WT, wild type
Recombinant Human Syt1 Proteins, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/syt+1/10__1096_slash_fj__201901028rr-38-0-4?v=OriGene
Average 90 stars, based on 1 article reviews
recombinant human syt1 proteins - by Bioz Stars, 2026-07
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syt1 plasmid  (OriGene)
92
OriGene syt1 plasmid
( A ) Representative confocal images of HEK293T cells expressing GB2-VC and Syt11-VN tagged with the C-terminal (VC) and N-terminal (VN) fragments of the fluorescent Venus protein (top row). Reconstitution of Venus fluorescence is observed only in cells expressing KCTD16. In control experiments, replacing Syt11-VN with Syt11ΔC2-VN lacking the C2A and C2B domains (middle row) or <t>Syt1-VN</t> (bottom row) does not reconstitute Venus fluorescence. Transfected cells were identified using mCherry. Scale bar: 10 μm. ( B ) Representative Western blots (left) and corresponding quantifications from n = 5 independent experiments (right) of APs with anti-HA antibodies from cell lysates of transfected HEK293T cells expressing the indicated constructs. AP and input lanes were probed with anti-Syt11 (top), anti-KCTD16 (middle), and anti-HA (bottom) antibodies. The presence of VN- or VC-tags on Syt11 and GB2, respectively, does not significantly alter the amounts of KCTD16 ( p = 0.436) and Syt11 ( p = 0.858) co-purified with GB2. Values are presented as mean ± SEM, ns = not significant, unpaired t-test.
Syt1 Plasmid, supplied by OriGene, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/syt+1/pmc11169412-270-1-5?v=OriGene
Average 92 stars, based on 1 article reviews
syt1 plasmid - by Bioz Stars, 2026-07
92/100 stars
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pegfp c1 egfp esyt1  (Addgene inc)
92
Addgene inc pegfp c1 egfp esyt1
( A ) Representative confocal images of HEK293T cells expressing GB2-VC and Syt11-VN tagged with the C-terminal (VC) and N-terminal (VN) fragments of the fluorescent Venus protein (top row). Reconstitution of Venus fluorescence is observed only in cells expressing KCTD16. In control experiments, replacing Syt11-VN with Syt11ΔC2-VN lacking the C2A and C2B domains (middle row) or <t>Syt1-VN</t> (bottom row) does not reconstitute Venus fluorescence. Transfected cells were identified using mCherry. Scale bar: 10 μm. ( B ) Representative Western blots (left) and corresponding quantifications from n = 5 independent experiments (right) of APs with anti-HA antibodies from cell lysates of transfected HEK293T cells expressing the indicated constructs. AP and input lanes were probed with anti-Syt11 (top), anti-KCTD16 (middle), and anti-HA (bottom) antibodies. The presence of VN- or VC-tags on Syt11 and GB2, respectively, does not significantly alter the amounts of KCTD16 ( p = 0.436) and Syt11 ( p = 0.858) co-purified with GB2. Values are presented as mean ± SEM, ns = not significant, unpaired t-test.
Pegfp C1 Egfp Esyt1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/syt+1/pmc09457520__pnas__2202730119__sapp-1-20-27?v=Addgene+inc
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pegfp c1 egfp esyt1 - by Bioz Stars, 2026-07
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santa cruz sc  (Santa Cruz Biotechnology)
91
Santa Cruz Biotechnology santa cruz sc
( A ) Representative confocal images of HEK293T cells expressing GB2-VC and Syt11-VN tagged with the C-terminal (VC) and N-terminal (VN) fragments of the fluorescent Venus protein (top row). Reconstitution of Venus fluorescence is observed only in cells expressing KCTD16. In control experiments, replacing Syt11-VN with Syt11ΔC2-VN lacking the C2A and C2B domains (middle row) or <t>Syt1-VN</t> (bottom row) does not reconstitute Venus fluorescence. Transfected cells were identified using mCherry. Scale bar: 10 μm. ( B ) Representative Western blots (left) and corresponding quantifications from n = 5 independent experiments (right) of APs with anti-HA antibodies from cell lysates of transfected HEK293T cells expressing the indicated constructs. AP and input lanes were probed with anti-Syt11 (top), anti-KCTD16 (middle), and anti-HA (bottom) antibodies. The presence of VN- or VC-tags on Syt11 and GB2, respectively, does not significantly alter the amounts of KCTD16 ( p = 0.436) and Syt11 ( p = 0.858) co-purified with GB2. Values are presented as mean ± SEM, ns = not significant, unpaired t-test.
Santa Cruz Sc, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/syt+1/pmc12573374__gutjnl-74-11-s011-0-9-9?v=Santa+Cruz+Biotechnology
Average 91 stars, based on 1 article reviews
santa cruz sc - by Bioz Stars, 2026-07
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rabbit anti syn  (Proteintech)
94
Proteintech rabbit anti syn
( A ) Representative confocal images of HEK293T cells expressing GB2-VC and Syt11-VN tagged with the C-terminal (VC) and N-terminal (VN) fragments of the fluorescent Venus protein (top row). Reconstitution of Venus fluorescence is observed only in cells expressing KCTD16. In control experiments, replacing Syt11-VN with Syt11ΔC2-VN lacking the C2A and C2B domains (middle row) or <t>Syt1-VN</t> (bottom row) does not reconstitute Venus fluorescence. Transfected cells were identified using mCherry. Scale bar: 10 μm. ( B ) Representative Western blots (left) and corresponding quantifications from n = 5 independent experiments (right) of APs with anti-HA antibodies from cell lysates of transfected HEK293T cells expressing the indicated constructs. AP and input lanes were probed with anti-Syt11 (top), anti-KCTD16 (middle), and anti-HA (bottom) antibodies. The presence of VN- or VC-tags on Syt11 and GB2, respectively, does not significantly alter the amounts of KCTD16 ( p = 0.436) and Syt11 ( p = 0.858) co-purified with GB2. Values are presented as mean ± SEM, ns = not significant, unpaired t-test.
Rabbit Anti Syn, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/syt+1/pmc12158875__12264_2025_1376_MOESM1_ESM-55-37-39?v=Proteintech
Average 94 stars, based on 1 article reviews
rabbit anti syn - by Bioz Stars, 2026-07
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gene exp syt1 hs00194572 m1  (Thermo Fisher)
98
Thermo Fisher gene exp syt1 hs00194572 m1
( A ) Representative confocal images of HEK293T cells expressing GB2-VC and Syt11-VN tagged with the C-terminal (VC) and N-terminal (VN) fragments of the fluorescent Venus protein (top row). Reconstitution of Venus fluorescence is observed only in cells expressing KCTD16. In control experiments, replacing Syt11-VN with Syt11ΔC2-VN lacking the C2A and C2B domains (middle row) or <t>Syt1-VN</t> (bottom row) does not reconstitute Venus fluorescence. Transfected cells were identified using mCherry. Scale bar: 10 μm. ( B ) Representative Western blots (left) and corresponding quantifications from n = 5 independent experiments (right) of APs with anti-HA antibodies from cell lysates of transfected HEK293T cells expressing the indicated constructs. AP and input lanes were probed with anti-Syt11 (top), anti-KCTD16 (middle), and anti-HA (bottom) antibodies. The presence of VN- or VC-tags on Syt11 and GB2, respectively, does not significantly alter the amounts of KCTD16 ( p = 0.436) and Syt11 ( p = 0.858) co-purified with GB2. Values are presented as mean ± SEM, ns = not significant, unpaired t-test.
Gene Exp Syt1 Hs00194572 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/syt+1/pmc02790617__ppat__1000702__s003-0-25--1?v=Thermo+Fisher
Average 98 stars, based on 1 article reviews
gene exp syt1 hs00194572 m1 - by Bioz Stars, 2026-07
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anti nuclear factor kappa b nf κb p65 p65 f 6 mouse mab  (Alomone Labs)
90
Alomone Labs anti nuclear factor kappa b nf κb p65 p65 f 6 mouse mab
( A ) Representative confocal images of HEK293T cells expressing GB2-VC and Syt11-VN tagged with the C-terminal (VC) and N-terminal (VN) fragments of the fluorescent Venus protein (top row). Reconstitution of Venus fluorescence is observed only in cells expressing KCTD16. In control experiments, replacing Syt11-VN with Syt11ΔC2-VN lacking the C2A and C2B domains (middle row) or <t>Syt1-VN</t> (bottom row) does not reconstitute Venus fluorescence. Transfected cells were identified using mCherry. Scale bar: 10 μm. ( B ) Representative Western blots (left) and corresponding quantifications from n = 5 independent experiments (right) of APs with anti-HA antibodies from cell lysates of transfected HEK293T cells expressing the indicated constructs. AP and input lanes were probed with anti-Syt11 (top), anti-KCTD16 (middle), and anti-HA (bottom) antibodies. The presence of VN- or VC-tags on Syt11 and GB2, respectively, does not significantly alter the amounts of KCTD16 ( p = 0.436) and Syt11 ( p = 0.858) co-purified with GB2. Values are presented as mean ± SEM, ns = not significant, unpaired t-test.
Anti Nuclear Factor Kappa B Nf κb P65 P65 F 6 Mouse Mab, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/syt+1/pm32077170-33-20-48?v=Alomone+Labs
Average 90 stars, based on 1 article reviews
anti nuclear factor kappa b nf κb p65 p65 f 6 mouse mab - by Bioz Stars, 2026-07
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human e syt1  (OriGene)
92
OriGene human e syt1
( A ) Representative confocal images of HEK293T cells expressing GB2-VC and Syt11-VN tagged with the C-terminal (VC) and N-terminal (VN) fragments of the fluorescent Venus protein (top row). Reconstitution of Venus fluorescence is observed only in cells expressing KCTD16. In control experiments, replacing Syt11-VN with Syt11ΔC2-VN lacking the C2A and C2B domains (middle row) or <t>Syt1-VN</t> (bottom row) does not reconstitute Venus fluorescence. Transfected cells were identified using mCherry. Scale bar: 10 μm. ( B ) Representative Western blots (left) and corresponding quantifications from n = 5 independent experiments (right) of APs with anti-HA antibodies from cell lysates of transfected HEK293T cells expressing the indicated constructs. AP and input lanes were probed with anti-Syt11 (top), anti-KCTD16 (middle), and anti-HA (bottom) antibodies. The presence of VN- or VC-tags on Syt11 and GB2, respectively, does not significantly alter the amounts of KCTD16 ( p = 0.436) and Syt11 ( p = 0.858) co-purified with GB2. Values are presented as mean ± SEM, ns = not significant, unpaired t-test.
Human E Syt1, supplied by OriGene, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/syt+1/pmc05770786-609-21-38?v=OriGene
Average 92 stars, based on 1 article reviews
human e syt1 - by Bioz Stars, 2026-07
92/100 stars
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gene exp syt1 rn00436862 m1  (Thermo Fisher)
86
Thermo Fisher gene exp syt1 rn00436862 m1
( A ) Representative confocal images of HEK293T cells expressing GB2-VC and Syt11-VN tagged with the C-terminal (VC) and N-terminal (VN) fragments of the fluorescent Venus protein (top row). Reconstitution of Venus fluorescence is observed only in cells expressing KCTD16. In control experiments, replacing Syt11-VN with Syt11ΔC2-VN lacking the C2A and C2B domains (middle row) or <t>Syt1-VN</t> (bottom row) does not reconstitute Venus fluorescence. Transfected cells were identified using mCherry. Scale bar: 10 μm. ( B ) Representative Western blots (left) and corresponding quantifications from n = 5 independent experiments (right) of APs with anti-HA antibodies from cell lysates of transfected HEK293T cells expressing the indicated constructs. AP and input lanes were probed with anti-Syt11 (top), anti-KCTD16 (middle), and anti-HA (bottom) antibodies. The presence of VN- or VC-tags on Syt11 and GB2, respectively, does not significantly alter the amounts of KCTD16 ( p = 0.436) and Syt11 ( p = 0.858) co-purified with GB2. Values are presented as mean ± SEM, ns = not significant, unpaired t-test.
Gene Exp Syt1 Rn00436862 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/syt+1/pmc04485591-221-21--1?v=Thermo+Fisher
Average 86 stars, based on 1 article reviews
gene exp syt1 rn00436862 m1 - by Bioz Stars, 2026-07
86/100 stars
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e syt1  (Santa Cruz Biotechnology)
92
Santa Cruz Biotechnology e syt1
( A ) Representative confocal images of HEK293T cells expressing GB2-VC and Syt11-VN tagged with the C-terminal (VC) and N-terminal (VN) fragments of the fluorescent Venus protein (top row). Reconstitution of Venus fluorescence is observed only in cells expressing KCTD16. In control experiments, replacing Syt11-VN with Syt11ΔC2-VN lacking the C2A and C2B domains (middle row) or <t>Syt1-VN</t> (bottom row) does not reconstitute Venus fluorescence. Transfected cells were identified using mCherry. Scale bar: 10 μm. ( B ) Representative Western blots (left) and corresponding quantifications from n = 5 independent experiments (right) of APs with anti-HA antibodies from cell lysates of transfected HEK293T cells expressing the indicated constructs. AP and input lanes were probed with anti-Syt11 (top), anti-KCTD16 (middle), and anti-HA (bottom) antibodies. The presence of VN- or VC-tags on Syt11 and GB2, respectively, does not significantly alter the amounts of KCTD16 ( p = 0.436) and Syt11 ( p = 0.858) co-purified with GB2. Values are presented as mean ± SEM, ns = not significant, unpaired t-test.
E Syt1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/syt+1/pmc11811301-36-0-2?v=Santa+Cruz+Biotechnology
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e syt1 - by Bioz Stars, 2026-07
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e syt1 rabbit polyclonal  (Bethyl)
88
Bethyl e syt1 rabbit polyclonal
Fig. 3: <t>E-Syt1</t> interacts with HSV-1gM in infected cells. HeLa cells were infected 728
E Syt1 Rabbit Polyclonal, supplied by Bethyl, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/syt+1/10__1128_slash_jvi__01281___17-170-40-46?v=Bethyl
Average 88 stars, based on 1 article reviews
e syt1 rabbit polyclonal - by Bioz Stars, 2026-07
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Image Search Results


FIGURE 3 Levels of neuronal markers were not altered in 18-month-old AppNL-F/wt knock-in; ob/ob mice. Levels of NF-H (A), SYT1 (B), PSD95 (C), and phospho-tau (D) were assessed using ELISAs and compared among each genotype after adjusting for sex (n = 10-44 mice/group). Standard curve of ELISAs of NF-H (E), SYT1 (F), PSD95 (G), and phospho-tau (H) with sample range as shown in red line in a representative assay. A-D, Data are presented as adjusted means ± standard errors of the means and were compared among each genotype using Tukey's HSD test. APP KI, AppNL-F/wt knock-in; APP KI ob/ob, AppNL-F/wt knock-in; ob/ob mice; NS, not significant; WT, wild type

Journal: The FASEB Journal

Article Title: Increased levels of Aβ42 decrease the lifespan of ob/ob mice with dysregulation of microglia and astrocytes

doi: 10.1096/fj.201901028rr

Figure Lengend Snippet: FIGURE 3 Levels of neuronal markers were not altered in 18-month-old AppNL-F/wt knock-in; ob/ob mice. Levels of NF-H (A), SYT1 (B), PSD95 (C), and phospho-tau (D) were assessed using ELISAs and compared among each genotype after adjusting for sex (n = 10-44 mice/group). Standard curve of ELISAs of NF-H (E), SYT1 (F), PSD95 (G), and phospho-tau (H) with sample range as shown in red line in a representative assay. A-D, Data are presented as adjusted means ± standard errors of the means and were compared among each genotype using Tukey's HSD test. APP KI, AppNL-F/wt knock-in; APP KI ob/ob, AppNL-F/wt knock-in; ob/ob mice; NS, not significant; WT, wild type

Article Snippet: Recombinant human SYT1 proteins (Origene) were used as standards.

Techniques: Knock-In

FIGURE 5 Levels of neuronal and glial markers in young (6-month-old) AppNL-F/wt knock-in; ob/ob mice. A, Body weight of young mice were compared among each genotype after adjusting for sex (n = 8-17 mice/group). B-F, Levels of NF-H (B), SYT1 (C), PSD95 (D), CD11b (E), and GFAP (F) in the brains of young mice were compared among each genotype after adjusting for sex (n = 9-17 mice/group). Data are presented as adjusted means ± standard errors of the means. *P < .05, **P < .01, and ***P < .001 for the comparisons among each genotype using Tukey's HSD test. APP KI, AppNL-F/wt knock-in; APP KI ob/ob, AppNL-F/wt knock-in; ob/ob mice; NS, not significant; WT, wild type

Journal: The FASEB Journal

Article Title: Increased levels of Aβ42 decrease the lifespan of ob/ob mice with dysregulation of microglia and astrocytes

doi: 10.1096/fj.201901028rr

Figure Lengend Snippet: FIGURE 5 Levels of neuronal and glial markers in young (6-month-old) AppNL-F/wt knock-in; ob/ob mice. A, Body weight of young mice were compared among each genotype after adjusting for sex (n = 8-17 mice/group). B-F, Levels of NF-H (B), SYT1 (C), PSD95 (D), CD11b (E), and GFAP (F) in the brains of young mice were compared among each genotype after adjusting for sex (n = 9-17 mice/group). Data are presented as adjusted means ± standard errors of the means. *P < .05, **P < .01, and ***P < .001 for the comparisons among each genotype using Tukey's HSD test. APP KI, AppNL-F/wt knock-in; APP KI ob/ob, AppNL-F/wt knock-in; ob/ob mice; NS, not significant; WT, wild type

Article Snippet: Recombinant human SYT1 proteins (Origene) were used as standards.

Techniques: Knock-In

( A ) Representative confocal images of HEK293T cells expressing GB2-VC and Syt11-VN tagged with the C-terminal (VC) and N-terminal (VN) fragments of the fluorescent Venus protein (top row). Reconstitution of Venus fluorescence is observed only in cells expressing KCTD16. In control experiments, replacing Syt11-VN with Syt11ΔC2-VN lacking the C2A and C2B domains (middle row) or Syt1-VN (bottom row) does not reconstitute Venus fluorescence. Transfected cells were identified using mCherry. Scale bar: 10 μm. ( B ) Representative Western blots (left) and corresponding quantifications from n = 5 independent experiments (right) of APs with anti-HA antibodies from cell lysates of transfected HEK293T cells expressing the indicated constructs. AP and input lanes were probed with anti-Syt11 (top), anti-KCTD16 (middle), and anti-HA (bottom) antibodies. The presence of VN- or VC-tags on Syt11 and GB2, respectively, does not significantly alter the amounts of KCTD16 ( p = 0.436) and Syt11 ( p = 0.858) co-purified with GB2. Values are presented as mean ± SEM, ns = not significant, unpaired t-test.

Journal: EMBO Reports

Article Title: Synaptotagmin-11 facilitates assembly of a presynaptic signaling complex in post-Golgi cargo vesicles

doi: 10.1038/s44319-024-00147-0

Figure Lengend Snippet: ( A ) Representative confocal images of HEK293T cells expressing GB2-VC and Syt11-VN tagged with the C-terminal (VC) and N-terminal (VN) fragments of the fluorescent Venus protein (top row). Reconstitution of Venus fluorescence is observed only in cells expressing KCTD16. In control experiments, replacing Syt11-VN with Syt11ΔC2-VN lacking the C2A and C2B domains (middle row) or Syt1-VN (bottom row) does not reconstitute Venus fluorescence. Transfected cells were identified using mCherry. Scale bar: 10 μm. ( B ) Representative Western blots (left) and corresponding quantifications from n = 5 independent experiments (right) of APs with anti-HA antibodies from cell lysates of transfected HEK293T cells expressing the indicated constructs. AP and input lanes were probed with anti-Syt11 (top), anti-KCTD16 (middle), and anti-HA (bottom) antibodies. The presence of VN- or VC-tags on Syt11 and GB2, respectively, does not significantly alter the amounts of KCTD16 ( p = 0.436) and Syt11 ( p = 0.858) co-purified with GB2. Values are presented as mean ± SEM, ns = not significant, unpaired t-test.

Article Snippet: The Syt1 plasmid was from OriGene (Cat# MR206688), pcDNA3.1-mCherry from Addgene (Cat# 128744), NPY-mCherry from Addgene (Cat# 67156) and pSI-AAR6-Rab5a-mcherry was a gift from M. Spiess (Kalin et al, ).

Techniques: Expressing, Fluorescence, Control, Transfection, Western Blot, Construct, Purification

Fig. 3: E-Syt1 interacts with HSV-1gM in infected cells. HeLa cells were infected 728

Journal: Journal of Virology

Article Title: Extended Synaptotagmin 1 Interacts with Herpes Simplex Virus 1 Glycoprotein M and Negatively Modulates Virus-Induced Membrane Fusion

doi: 10.1128/jvi.01281-17

Figure Lengend Snippet: Fig. 3: E-Syt1 interacts with HSV-1gM in infected cells. HeLa cells were infected 728

Article Snippet: Primary antibodies and dilution used in this study were as follows: anti-336 myc rabbit polyclonal (1:1000; 2272, Cell Signaling Technology), anti-myc mouse 337 polyclonal ( 9B11) (1:100; 2276, Cell Signaling Technology) anti-HA mouse monoclonal 338 (1:1000; SC-7392, Santa Cruz), anti E-Syt1 rabbit polyclonal (1:1000; A303-362A, 339 Bethyl Laboratories), anti E-Syt2 rabbit polyclonal (1:1000; NBP1-59988, Novus 340 Biologicals), anti E-Syt3 rabbit polyclonal (1:1000; NBP1-91354, Novus Biologicals), 341 anti β-actin mouse monoclonal (1:2500; ab6276, Abcam), anti γ-Tubulin mouse 342 monoclonal (1:5000; T6557, Sigma-Aldrich), anti-HSV VP5 mouse monoclonal (1:2000; 343 Virusys), anti-HSV gM rabbit polyclonal PAS980 (1:1000; courtesy of Dr. Lynn 344 Enquist), anti-HSV gM rabbit polyclonal 4c10 (1:000; courtesy of Dr. Joel Baines) and 345 anti-human DDX3 rabbit R648 polyclonal (1:4000; courtesy of Dr. A. Patel).

Techniques: Infection

Fig. 5: Impact of the virus on E-Syt1 expression. HeLa cells were mock treated or 750

Journal: Journal of Virology

Article Title: Extended Synaptotagmin 1 Interacts with Herpes Simplex Virus 1 Glycoprotein M and Negatively Modulates Virus-Induced Membrane Fusion

doi: 10.1128/jvi.01281-17

Figure Lengend Snippet: Fig. 5: Impact of the virus on E-Syt1 expression. HeLa cells were mock treated or 750

Article Snippet: Primary antibodies and dilution used in this study were as follows: anti-336 myc rabbit polyclonal (1:1000; 2272, Cell Signaling Technology), anti-myc mouse 337 polyclonal ( 9B11) (1:100; 2276, Cell Signaling Technology) anti-HA mouse monoclonal 338 (1:1000; SC-7392, Santa Cruz), anti E-Syt1 rabbit polyclonal (1:1000; A303-362A, 339 Bethyl Laboratories), anti E-Syt2 rabbit polyclonal (1:1000; NBP1-59988, Novus 340 Biologicals), anti E-Syt3 rabbit polyclonal (1:1000; NBP1-91354, Novus Biologicals), 341 anti β-actin mouse monoclonal (1:2500; ab6276, Abcam), anti γ-Tubulin mouse 342 monoclonal (1:5000; T6557, Sigma-Aldrich), anti-HSV VP5 mouse monoclonal (1:2000; 343 Virusys), anti-HSV gM rabbit polyclonal PAS980 (1:1000; courtesy of Dr. Lynn 344 Enquist), anti-HSV gM rabbit polyclonal 4c10 (1:000; courtesy of Dr. Joel Baines) and 345 anti-human DDX3 rabbit R648 polyclonal (1:4000; courtesy of Dr. A. Patel).

Techniques: Virus, Expressing

Fig. 9: E-Syt1 and E-Syt3 down regulation increases cell-to-cell spread. The 798

Journal: Journal of Virology

Article Title: Extended Synaptotagmin 1 Interacts with Herpes Simplex Virus 1 Glycoprotein M and Negatively Modulates Virus-Induced Membrane Fusion

doi: 10.1128/jvi.01281-17

Figure Lengend Snippet: Fig. 9: E-Syt1 and E-Syt3 down regulation increases cell-to-cell spread. The 798

Article Snippet: Primary antibodies and dilution used in this study were as follows: anti-336 myc rabbit polyclonal (1:1000; 2272, Cell Signaling Technology), anti-myc mouse 337 polyclonal ( 9B11) (1:100; 2276, Cell Signaling Technology) anti-HA mouse monoclonal 338 (1:1000; SC-7392, Santa Cruz), anti E-Syt1 rabbit polyclonal (1:1000; A303-362A, 339 Bethyl Laboratories), anti E-Syt2 rabbit polyclonal (1:1000; NBP1-59988, Novus 340 Biologicals), anti E-Syt3 rabbit polyclonal (1:1000; NBP1-91354, Novus Biologicals), 341 anti β-actin mouse monoclonal (1:2500; ab6276, Abcam), anti γ-Tubulin mouse 342 monoclonal (1:5000; T6557, Sigma-Aldrich), anti-HSV VP5 mouse monoclonal (1:2000; 343 Virusys), anti-HSV gM rabbit polyclonal PAS980 (1:1000; courtesy of Dr. Lynn 344 Enquist), anti-HSV gM rabbit polyclonal 4c10 (1:000; courtesy of Dr. Joel Baines) and 345 anti-human DDX3 rabbit R648 polyclonal (1:4000; courtesy of Dr. A. Patel).

Techniques:

Fig. 11: Overexpression of E-Syt1 or E-Syt-3 reduces viral yields. HeLa cells 818

Journal: Journal of Virology

Article Title: Extended Synaptotagmin 1 Interacts with Herpes Simplex Virus 1 Glycoprotein M and Negatively Modulates Virus-Induced Membrane Fusion

doi: 10.1128/jvi.01281-17

Figure Lengend Snippet: Fig. 11: Overexpression of E-Syt1 or E-Syt-3 reduces viral yields. HeLa cells 818

Article Snippet: Primary antibodies and dilution used in this study were as follows: anti-336 myc rabbit polyclonal (1:1000; 2272, Cell Signaling Technology), anti-myc mouse 337 polyclonal ( 9B11) (1:100; 2276, Cell Signaling Technology) anti-HA mouse monoclonal 338 (1:1000; SC-7392, Santa Cruz), anti E-Syt1 rabbit polyclonal (1:1000; A303-362A, 339 Bethyl Laboratories), anti E-Syt2 rabbit polyclonal (1:1000; NBP1-59988, Novus 340 Biologicals), anti E-Syt3 rabbit polyclonal (1:1000; NBP1-91354, Novus Biologicals), 341 anti β-actin mouse monoclonal (1:2500; ab6276, Abcam), anti γ-Tubulin mouse 342 monoclonal (1:5000; T6557, Sigma-Aldrich), anti-HSV VP5 mouse monoclonal (1:2000; 343 Virusys), anti-HSV gM rabbit polyclonal PAS980 (1:1000; courtesy of Dr. Lynn 344 Enquist), anti-HSV gM rabbit polyclonal 4c10 (1:000; courtesy of Dr. Joel Baines) and 345 anti-human DDX3 rabbit R648 polyclonal (1:4000; courtesy of Dr. A. Patel).

Techniques: Over Expression

Fig. 13: E-Syt1 and E-Syt3 knockdown increase cell-to-cell fusion in infected 843

Journal: Journal of Virology

Article Title: Extended Synaptotagmin 1 Interacts with Herpes Simplex Virus 1 Glycoprotein M and Negatively Modulates Virus-Induced Membrane Fusion

doi: 10.1128/jvi.01281-17

Figure Lengend Snippet: Fig. 13: E-Syt1 and E-Syt3 knockdown increase cell-to-cell fusion in infected 843

Article Snippet: Primary antibodies and dilution used in this study were as follows: anti-336 myc rabbit polyclonal (1:1000; 2272, Cell Signaling Technology), anti-myc mouse 337 polyclonal ( 9B11) (1:100; 2276, Cell Signaling Technology) anti-HA mouse monoclonal 338 (1:1000; SC-7392, Santa Cruz), anti E-Syt1 rabbit polyclonal (1:1000; A303-362A, 339 Bethyl Laboratories), anti E-Syt2 rabbit polyclonal (1:1000; NBP1-59988, Novus 340 Biologicals), anti E-Syt3 rabbit polyclonal (1:1000; NBP1-91354, Novus Biologicals), 341 anti β-actin mouse monoclonal (1:2500; ab6276, Abcam), anti γ-Tubulin mouse 342 monoclonal (1:5000; T6557, Sigma-Aldrich), anti-HSV VP5 mouse monoclonal (1:2000; 343 Virusys), anti-HSV gM rabbit polyclonal PAS980 (1:1000; courtesy of Dr. Lynn 344 Enquist), anti-HSV gM rabbit polyclonal 4c10 (1:000; courtesy of Dr. Joel Baines) and 345 anti-human DDX3 rabbit R648 polyclonal (1:4000; courtesy of Dr. A. Patel).

Techniques: Knockdown, Infection