Journal: The FASEB Journal

Article Title: Increased levels of Aβ42 decrease the lifespan of ob/ob mice with dysregulation of microglia and astrocytes

doi: 10.1096/fj.201901028rr

Figure Lengend Snippet: FIGURE 3 Levels of neuronal markers were not altered in 18-month-old AppNL-F/wt knock-in; ob/ob mice. Levels of NF-H (A), SYT1 (B), PSD95 (C), and phospho-tau (D) were assessed using ELISAs and compared among each genotype after adjusting for sex (n = 10-44 mice/group). Standard curve of ELISAs of NF-H (E), SYT1 (F), PSD95 (G), and phospho-tau (H) with sample range as shown in red line in a representative assay. A-D, Data are presented as adjusted means ± standard errors of the means and were compared among each genotype using Tukey's HSD test. APP KI, AppNL-F/wt knock-in; APP KI ob/ob, AppNL-F/wt knock-in; ob/ob mice; NS, not significant; WT, wild type

Article Snippet: Recombinant human SYT1 proteins (Origene) were used as standards.

Techniques: Knock-In

Journal: The FASEB Journal

Article Title: Increased levels of Aβ42 decrease the lifespan of ob/ob mice with dysregulation of microglia and astrocytes

doi: 10.1096/fj.201901028rr

Figure Lengend Snippet: FIGURE 5 Levels of neuronal and glial markers in young (6-month-old) AppNL-F/wt knock-in; ob/ob mice. A, Body weight of young mice were compared among each genotype after adjusting for sex (n = 8-17 mice/group). B-F, Levels of NF-H (B), SYT1 (C), PSD95 (D), CD11b (E), and GFAP (F) in the brains of young mice were compared among each genotype after adjusting for sex (n = 9-17 mice/group). Data are presented as adjusted means ± standard errors of the means. *P < .05, **P < .01, and ***P < .001 for the comparisons among each genotype using Tukey's HSD test. APP KI, AppNL-F/wt knock-in; APP KI ob/ob, AppNL-F/wt knock-in; ob/ob mice; NS, not significant; WT, wild type

Article Snippet: Recombinant human SYT1 proteins (Origene) were used as standards.

Techniques: Knock-In

Journal: EMBO Reports

Article Title: Synaptotagmin-11 facilitates assembly of a presynaptic signaling complex in post-Golgi cargo vesicles

doi: 10.1038/s44319-024-00147-0

Figure Lengend Snippet: ( A ) Representative confocal images of HEK293T cells expressing GB2-VC and Syt11-VN tagged with the C-terminal (VC) and N-terminal (VN) fragments of the fluorescent Venus protein (top row). Reconstitution of Venus fluorescence is observed only in cells expressing KCTD16. In control experiments, replacing Syt11-VN with Syt11ΔC2-VN lacking the C2A and C2B domains (middle row) or Syt1-VN (bottom row) does not reconstitute Venus fluorescence. Transfected cells were identified using mCherry. Scale bar: 10 μm. ( B ) Representative Western blots (left) and corresponding quantifications from n = 5 independent experiments (right) of APs with anti-HA antibodies from cell lysates of transfected HEK293T cells expressing the indicated constructs. AP and input lanes were probed with anti-Syt11 (top), anti-KCTD16 (middle), and anti-HA (bottom) antibodies. The presence of VN- or VC-tags on Syt11 and GB2, respectively, does not significantly alter the amounts of KCTD16 ( p = 0.436) and Syt11 ( p = 0.858) co-purified with GB2. Values are presented as mean ± SEM, ns = not significant, unpaired t-test.

Article Snippet: The Syt1 plasmid was from OriGene (Cat# MR206688), pcDNA3.1-mCherry from Addgene (Cat# 128744), NPY-mCherry from Addgene (Cat# 67156) and pSI-AAR6-Rab5a-mcherry was a gift from M. Spiess (Kalin et al, ).

Techniques: Expressing, Fluorescence, Control, Transfection, Western Blot, Construct, Purification