Journal: Experimental Biology and Medicine

Article Title: Pulmonary injury following exposure to amorphous silicon dioxide nanoparticles in Golden Syrian Hamsters

doi: 10.3389/ebm.2026.10793

Figure Lengend Snippet: Total Cells and leukocytes in the bronchoalveolar lavage fluid (BALF) of Golden Syrian Hamsters were counted to determine if inflammation was occurring after exposure to inhaled SiO 2 NPs. The cells were re-suspended in 1 mL of RPMI media followed by determination of total cell numbers (A) . Total Cell numbers (A) were significantly increased by 16-fold in the 12 mg/m 3 treated group. Differential cell count (B–F) slide smears were prepared from the BALF of cells from the lung exposed to 6 and 12 mg/m 3 SiO 2 NPs for 4h/day for 8 days. Macrophages (B) of treated groups were not changed when compared to control. Neutrophils (C) , Eosinophils (D) , Lymphocytes (E) , and Multinucleated Macrophages (F) were significantly increased in the 12 mg/m 3 treated group when compared to control. N = 5.

Article Snippet: Female Golden Syrian Hamsters, age 7–8 weeks were obtained from Envigo (Indianapolis, IN).

Techniques: Cell Characterization, Control

Journal: Experimental Biology and Medicine

Article Title: Pulmonary injury following exposure to amorphous silicon dioxide nanoparticles in Golden Syrian Hamsters

doi: 10.3389/ebm.2026.10793

Figure Lengend Snippet: Markers measured in the BALF of Golden Syrian Hamsters from lungs exposed to 6 and 12 mg/m 3 SiO 2 NPs for 4h/day for 8 days were Total Protein (TP) Alkaline Phosphatase (ALP), and Lactate Dehydrogenase (LDH). (A) TP, (B) ALP), and (C) LDH were all significantly increased in the 12 mg/m 3 treated group when compared to vehicle control by 4-fold, 9-fold and 3-fold, respectively. N = 4.

Article Snippet: Female Golden Syrian Hamsters, age 7–8 weeks were obtained from Envigo (Indianapolis, IN).

Techniques: Control

Journal: Infection and Immunity

Article Title: Vaccination against the Intracellular Pathogens Leishmania major and L. amazonensis by Directing CD40 Ligand to Macrophages

doi: 10.1128/iai.69.5.3255-3263.2001

Figure Lengend Snippet: FIG. 4. The expression of antigens on the surface of transfected L929 cells. Transfected L cells were incubated with MR1 MAb to CD40L or MAb #96 to Leishmania gp63 and were stained with FITC-conjugated anti-IgG, as described in Materials and Methods. After being extensively washed, cells were fixed in 1% paraformaldehyde in PBS and analyzed on an Epics Elite flow cytometer. Profiles of cells stained with each antibody were overlaid and compared to those of cells stained with secondary antibody alone.

Article Snippet: After 45 min at 4°C in the primary antibody, the cells were washed with cold buffer and incubated with either fluorescein isothiocyanate (FITC)-conjugated goat anti-hamster IgG (Jackson ImmunoResearch, West Grove, Pa.) for CD40L or FITC-conjugated goat anti-mouse IgG (Jackson ImmunoResearch) for gp63 for 45 min at 4°C.

Techniques: Expressing, Transfection, Incubation, Staining, Cytometry