synthetic positive control Search Results


synthetic positive control template for standard curve  (PrimerDesign Inc)
90
PrimerDesign Inc synthetic positive control template for standard curve
Synthetic Positive Control Template For Standard Curve, supplied by PrimerDesign Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/synthetic+positive+control/synthetic+positive+control+template+for+standard+curve/10__1094_slash_pdis___06___18___1101___re-103-19-21
Average 90 stars, based on 1 article reviews
synthetic positive control template for standard curve - by Bioz Stars, 2026-09
90/100 stars
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synthetic wnv positive control  (GenScript corporation)
90
GenScript corporation synthetic wnv positive control
Synthetic Wnv Positive Control, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/synthetic+positive+control/synthetic+wnv+positive+control/pm27881079-70-17-21
Average 90 stars, based on 1 article reviews
synthetic wnv positive control - by Bioz Stars, 2026-09
90/100 stars
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synthetic positive control protein  (GenScript corporation)
90
GenScript corporation synthetic positive control protein
Synthetic Positive Control Protein, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/synthetic+positive+control/synthetic+positive+control+protein/pmc04083859-152-10-24
Average 90 stars, based on 1 article reviews
synthetic positive control protein - by Bioz Stars, 2026-09
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synthetic positive control protein containing a cellulose-binding domain (cbd) and a c-myc tag  (GenScript corporation)
90
GenScript corporation synthetic positive control protein containing a cellulose-binding domain (cbd) and a c-myc tag
Synthetic Positive Control Protein Containing A Cellulose Binding Domain (Cbd) And A C Myc Tag, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/synthetic+positive+control/synthetic+positive+control+protein+containing+a+cellulose+binding+domain++cbd++and+a+c+myc+tag/pmc06976532-91-18-27
Average 90 stars, based on 1 article reviews
synthetic positive control protein containing a cellulose-binding domain (cbd) and a c-myc tag - by Bioz Stars, 2026-09
90/100 stars
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synthetic positive control protein containing a cellulose-binding domain and a cmyc tag  (GenScript corporation)
90
GenScript corporation synthetic positive control protein containing a cellulose-binding domain and a cmyc tag
Synthetic Positive Control Protein Containing A Cellulose Binding Domain And A Cmyc Tag, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/synthetic+positive+control/synthetic+positive+control+protein+containing+a+cellulose+binding+domain+and+a+cmyc+tag/pm23915965-161-16-24
Average 90 stars, based on 1 article reviews
synthetic positive control protein containing a cellulose-binding domain and a cmyc tag - by Bioz Stars, 2026-09
90/100 stars
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synthetic rna positive control material seracare accuplex  (SeraCare Life Sciences)
90
SeraCare Life Sciences synthetic rna positive control material seracare accuplex
<t>DF-prepared</t> <t>MMIX</t> compared with manually-prepared MMIX endpoint RFU data from PCR reactions using dilutions of synthetic <t>RNA</t> positive control material (Twist) at and around the limit of detection. (A) ROX RFU kernel distribution and RFU counts. Dotted line represents the 1600 RFU cutoff. Dashed lines represent the arithmetic mean of ROX RFU. (B) Kernel Distribution and count for FAM RFU values. Dashed lines represent the arithmetic mean of FAM RFU. (C) Kernel Distribution and count for n_FAM values. Solid lines represent the “Inconclusive” PLOD (Blue) and “Detected” (Black) n_FAM cutoff for clinical calling. Dashed lines represent the arithmetic mean of n_FAM (D) Scatterplot n_FAM vs. ROX RFU. Data are pooled from six 384-well arrays for both conditions. Solid lines represent the “Inconclusive” PLOD (Blue) and “Detected” (Black) n_FAM cutoff for clinical calling. Dotted line represents the 1600 ROX RFU cutoff. Data are pooled from six 384-well arrays for both conditions. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
Synthetic Rna Positive Control Material Seracare Accuplex, supplied by SeraCare Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/synthetic+positive+control/synthetic+rna+positive+control+material+seracare+accuplex/pmc11639134-391-17-22
Average 90 stars, based on 1 article reviews
synthetic rna positive control material seracare accuplex - by Bioz Stars, 2026-09
90/100 stars
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synthetic positive control sample  (Microsynth ag)
90
Microsynth ag synthetic positive control sample
<t>DF-prepared</t> <t>MMIX</t> compared with manually-prepared MMIX endpoint RFU data from PCR reactions using dilutions of synthetic <t>RNA</t> positive control material (Twist) at and around the limit of detection. (A) ROX RFU kernel distribution and RFU counts. Dotted line represents the 1600 RFU cutoff. Dashed lines represent the arithmetic mean of ROX RFU. (B) Kernel Distribution and count for FAM RFU values. Dashed lines represent the arithmetic mean of FAM RFU. (C) Kernel Distribution and count for n_FAM values. Solid lines represent the “Inconclusive” PLOD (Blue) and “Detected” (Black) n_FAM cutoff for clinical calling. Dashed lines represent the arithmetic mean of n_FAM (D) Scatterplot n_FAM vs. ROX RFU. Data are pooled from six 384-well arrays for both conditions. Solid lines represent the “Inconclusive” PLOD (Blue) and “Detected” (Black) n_FAM cutoff for clinical calling. Dotted line represents the 1600 ROX RFU cutoff. Data are pooled from six 384-well arrays for both conditions. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
Synthetic Positive Control Sample, supplied by Microsynth ag, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/synthetic+positive+control/synthetic+positive+control+sample/pm36064726-97-12-16
Average 90 stars, based on 1 article reviews
synthetic positive control sample - by Bioz Stars, 2026-09
90/100 stars
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oxford nanopore a synthetically constructed artificial positive control (apc) (puc57-apc-hop)  (Oxford Nanopore)
90
Oxford Nanopore oxford nanopore a synthetically constructed artificial positive control (apc) (puc57-apc-hop)
<t>DF-prepared</t> <t>MMIX</t> compared with manually-prepared MMIX endpoint RFU data from PCR reactions using dilutions of synthetic <t>RNA</t> positive control material (Twist) at and around the limit of detection. (A) ROX RFU kernel distribution and RFU counts. Dotted line represents the 1600 RFU cutoff. Dashed lines represent the arithmetic mean of ROX RFU. (B) Kernel Distribution and count for FAM RFU values. Dashed lines represent the arithmetic mean of FAM RFU. (C) Kernel Distribution and count for n_FAM values. Solid lines represent the “Inconclusive” PLOD (Blue) and “Detected” (Black) n_FAM cutoff for clinical calling. Dashed lines represent the arithmetic mean of n_FAM (D) Scatterplot n_FAM vs. ROX RFU. Data are pooled from six 384-well arrays for both conditions. Solid lines represent the “Inconclusive” PLOD (Blue) and “Detected” (Black) n_FAM cutoff for clinical calling. Dotted line represents the 1600 ROX RFU cutoff. Data are pooled from six 384-well arrays for both conditions. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
Oxford Nanopore A Synthetically Constructed Artificial Positive Control (Apc) (Puc57 Apc Hop), supplied by Oxford Nanopore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/synthetic+positive+control/oxford+nanopore+a+synthetically+constructed+artificial+positive+control++apc+++puc57+apc+hop+/10__1094_slash_phytofr___09___24___0106___fi-91-4-4
Average 90 stars, based on 1 article reviews
oxford nanopore a synthetically constructed artificial positive control (apc) (puc57-apc-hop) - by Bioz Stars, 2026-09
90/100 stars
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synthetic kanamycin positive control rna  (Promega)
90
Promega synthetic kanamycin positive control rna
<t>DF-prepared</t> <t>MMIX</t> compared with manually-prepared MMIX endpoint RFU data from PCR reactions using dilutions of synthetic <t>RNA</t> positive control material (Twist) at and around the limit of detection. (A) ROX RFU kernel distribution and RFU counts. Dotted line represents the 1600 RFU cutoff. Dashed lines represent the arithmetic mean of ROX RFU. (B) Kernel Distribution and count for FAM RFU values. Dashed lines represent the arithmetic mean of FAM RFU. (C) Kernel Distribution and count for n_FAM values. Solid lines represent the “Inconclusive” PLOD (Blue) and “Detected” (Black) n_FAM cutoff for clinical calling. Dashed lines represent the arithmetic mean of n_FAM (D) Scatterplot n_FAM vs. ROX RFU. Data are pooled from six 384-well arrays for both conditions. Solid lines represent the “Inconclusive” PLOD (Blue) and “Detected” (Black) n_FAM cutoff for clinical calling. Dotted line represents the 1600 ROX RFU cutoff. Data are pooled from six 384-well arrays for both conditions. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
Synthetic Kanamycin Positive Control Rna, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/synthetic+positive+control/synthetic+kanamycin+positive+control+rna/pm25881898-82-0-5
Average 90 stars, based on 1 article reviews
synthetic kanamycin positive control rna - by Bioz Stars, 2026-09
90/100 stars
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synthetic positive control peptides with a primary sequence of ftatdshtdtpealttk  (GenScript corporation)
90
GenScript corporation synthetic positive control peptides with a primary sequence of ftatdshtdtpealttk
<t>DF-prepared</t> <t>MMIX</t> compared with manually-prepared MMIX endpoint RFU data from PCR reactions using dilutions of synthetic <t>RNA</t> positive control material (Twist) at and around the limit of detection. (A) ROX RFU kernel distribution and RFU counts. Dotted line represents the 1600 RFU cutoff. Dashed lines represent the arithmetic mean of ROX RFU. (B) Kernel Distribution and count for FAM RFU values. Dashed lines represent the arithmetic mean of FAM RFU. (C) Kernel Distribution and count for n_FAM values. Solid lines represent the “Inconclusive” PLOD (Blue) and “Detected” (Black) n_FAM cutoff for clinical calling. Dashed lines represent the arithmetic mean of n_FAM (D) Scatterplot n_FAM vs. ROX RFU. Data are pooled from six 384-well arrays for both conditions. Solid lines represent the “Inconclusive” PLOD (Blue) and “Detected” (Black) n_FAM cutoff for clinical calling. Dotted line represents the 1600 ROX RFU cutoff. Data are pooled from six 384-well arrays for both conditions. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
Synthetic Positive Control Peptides With A Primary Sequence Of Ftatdshtdtpealttk, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/synthetic+positive+control/synthetic+positive+control+peptides+with+a+primary+sequence+of+ftatdshtdtpealttk/10__1039_slash_c7an00757d-57-9-25
Average 90 stars, based on 1 article reviews
synthetic positive control peptides with a primary sequence of ftatdshtdtpealttk - by Bioz Stars, 2026-09
90/100 stars
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synthetic dna template positive controls  (PrimerDesign Inc)
90
PrimerDesign Inc synthetic dna template positive controls
<t>DF-prepared</t> <t>MMIX</t> compared with manually-prepared MMIX endpoint RFU data from PCR reactions using dilutions of synthetic <t>RNA</t> positive control material (Twist) at and around the limit of detection. (A) ROX RFU kernel distribution and RFU counts. Dotted line represents the 1600 RFU cutoff. Dashed lines represent the arithmetic mean of ROX RFU. (B) Kernel Distribution and count for FAM RFU values. Dashed lines represent the arithmetic mean of FAM RFU. (C) Kernel Distribution and count for n_FAM values. Solid lines represent the “Inconclusive” PLOD (Blue) and “Detected” (Black) n_FAM cutoff for clinical calling. Dashed lines represent the arithmetic mean of n_FAM (D) Scatterplot n_FAM vs. ROX RFU. Data are pooled from six 384-well arrays for both conditions. Solid lines represent the “Inconclusive” PLOD (Blue) and “Detected” (Black) n_FAM cutoff for clinical calling. Dotted line represents the 1600 ROX RFU cutoff. Data are pooled from six 384-well arrays for both conditions. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
Synthetic Dna Template Positive Controls, supplied by PrimerDesign Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/synthetic+positive+control/synthetic+dna+template+positive+controls/pm34232572-318-21-6
Average 90 stars, based on 1 article reviews
synthetic dna template positive controls - by Bioz Stars, 2026-09
90/100 stars
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internal positive control (ipc) synthetic dna  (Biomeme inc)
90
Biomeme inc internal positive control (ipc) synthetic dna
<t>DF-prepared</t> <t>MMIX</t> compared with manually-prepared MMIX endpoint RFU data from PCR reactions using dilutions of synthetic <t>RNA</t> positive control material (Twist) at and around the limit of detection. (A) ROX RFU kernel distribution and RFU counts. Dotted line represents the 1600 RFU cutoff. Dashed lines represent the arithmetic mean of ROX RFU. (B) Kernel Distribution and count for FAM RFU values. Dashed lines represent the arithmetic mean of FAM RFU. (C) Kernel Distribution and count for n_FAM values. Solid lines represent the “Inconclusive” PLOD (Blue) and “Detected” (Black) n_FAM cutoff for clinical calling. Dashed lines represent the arithmetic mean of n_FAM (D) Scatterplot n_FAM vs. ROX RFU. Data are pooled from six 384-well arrays for both conditions. Solid lines represent the “Inconclusive” PLOD (Blue) and “Detected” (Black) n_FAM cutoff for clinical calling. Dotted line represents the 1600 ROX RFU cutoff. Data are pooled from six 384-well arrays for both conditions. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
Internal Positive Control (Ipc) Synthetic Dna, supplied by Biomeme inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/synthetic+positive+control/internal+positive+control++ipc++synthetic+dna/pm30269364-139-11-13
Average 90 stars, based on 1 article reviews
internal positive control (ipc) synthetic dna - by Bioz Stars, 2026-09
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Image Search Results


DF-prepared MMIX compared with manually-prepared MMIX endpoint RFU data from PCR reactions using dilutions of synthetic RNA positive control material (Twist) at and around the limit of detection. (A) ROX RFU kernel distribution and RFU counts. Dotted line represents the 1600 RFU cutoff. Dashed lines represent the arithmetic mean of ROX RFU. (B) Kernel Distribution and count for FAM RFU values. Dashed lines represent the arithmetic mean of FAM RFU. (C) Kernel Distribution and count for n_FAM values. Solid lines represent the “Inconclusive” PLOD (Blue) and “Detected” (Black) n_FAM cutoff for clinical calling. Dashed lines represent the arithmetic mean of n_FAM (D) Scatterplot n_FAM vs. ROX RFU. Data are pooled from six 384-well arrays for both conditions. Solid lines represent the “Inconclusive” PLOD (Blue) and “Detected” (Black) n_FAM cutoff for clinical calling. Dotted line represents the 1600 ROX RFU cutoff. Data are pooled from six 384-well arrays for both conditions. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

Journal: Slas Technology

Article Title: Development and validation of automated methods for COVID-19 PCR Master Mix preparation

doi: 10.1016/j.slast.2024.100195

Figure Lengend Snippet: DF-prepared MMIX compared with manually-prepared MMIX endpoint RFU data from PCR reactions using dilutions of synthetic RNA positive control material (Twist) at and around the limit of detection. (A) ROX RFU kernel distribution and RFU counts. Dotted line represents the 1600 RFU cutoff. Dashed lines represent the arithmetic mean of ROX RFU. (B) Kernel Distribution and count for FAM RFU values. Dashed lines represent the arithmetic mean of FAM RFU. (C) Kernel Distribution and count for n_FAM values. Solid lines represent the “Inconclusive” PLOD (Blue) and “Detected” (Black) n_FAM cutoff for clinical calling. Dashed lines represent the arithmetic mean of n_FAM (D) Scatterplot n_FAM vs. ROX RFU. Data are pooled from six 384-well arrays for both conditions. Solid lines represent the “Inconclusive” PLOD (Blue) and “Detected” (Black) n_FAM cutoff for clinical calling. Dotted line represents the 1600 ROX RFU cutoff. Data are pooled from six 384-well arrays for both conditions. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: Fig. 10 STARlet-prepared MMIX compared with manually-prepared MMIX endpoint RFU data from PCR reactions using dilutions of synthetic RNA positive control material (Seracare AccuPlex) at and around the limit of detection. (A) ROX RFU kernel distribution and RFU counts.

Techniques: Positive Control

DF-prepared MMIX compared with manually-prepared MMIX after addition of 0.05% Tween-20. endpoint RFU data from PCR reactions using dilutions of synthetic RNA positive control material (Twist) at and around the limit of detection. (A) ROX RFU kernel distribution and RFU counts. Dotted line represents the 1600 ROX RFU cutoff. Dashed lines represent the arithmetic mean of ROX RFU. (B) Kernel Distribution and count for FAM RFU values. Dashed lines represent the arithmetic mean of FAM RFU. (C) Kernel Distribution and count for n_FAM values. Solid lines represent the “Inconclusive” PLOD (Blue) and “Detected” (Black) n_FAM cutoff for clinical calling. Dashed lines represent the arithmetic mean of n_FAM. (D) Scatterplot n_FAM vs. ROX RFU. Solid lines represent the “Inconclusive” PLOD (Blue) and “Detected” (Black) n_FAM cutoff for clinical calling. Dotted line represents the 1600 ROX RFU cutoff. Data are pooled from seven 384-well arrays for both conditions. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

Journal: Slas Technology

Article Title: Development and validation of automated methods for COVID-19 PCR Master Mix preparation

doi: 10.1016/j.slast.2024.100195

Figure Lengend Snippet: DF-prepared MMIX compared with manually-prepared MMIX after addition of 0.05% Tween-20. endpoint RFU data from PCR reactions using dilutions of synthetic RNA positive control material (Twist) at and around the limit of detection. (A) ROX RFU kernel distribution and RFU counts. Dotted line represents the 1600 ROX RFU cutoff. Dashed lines represent the arithmetic mean of ROX RFU. (B) Kernel Distribution and count for FAM RFU values. Dashed lines represent the arithmetic mean of FAM RFU. (C) Kernel Distribution and count for n_FAM values. Solid lines represent the “Inconclusive” PLOD (Blue) and “Detected” (Black) n_FAM cutoff for clinical calling. Dashed lines represent the arithmetic mean of n_FAM. (D) Scatterplot n_FAM vs. ROX RFU. Solid lines represent the “Inconclusive” PLOD (Blue) and “Detected” (Black) n_FAM cutoff for clinical calling. Dotted line represents the 1600 ROX RFU cutoff. Data are pooled from seven 384-well arrays for both conditions. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: Fig. 10 STARlet-prepared MMIX compared with manually-prepared MMIX endpoint RFU data from PCR reactions using dilutions of synthetic RNA positive control material (Seracare AccuPlex) at and around the limit of detection. (A) ROX RFU kernel distribution and RFU counts.

Techniques: Positive Control

STARlet-prepared MMIX compared with manually-prepared MMIX endpoint RFU data from PCR reactions using dilutions of synthetic RNA positive control material (Twist) at and around the limit of detection. (A) ROX RFU kernel distribution and RFU counts. Dotted line represents the 1600 ROX RFU cutoff. Dashed lines represent the arithmetic mean of ROX RFU. (B) Kernel Distribution and count for FAM RFU values. Dashed lines represent the arithmetic mean of FAM RFU. (C) Kernel Distribution and count for n_FAM values. Solid lines represent the “Inconclusive” PLOD (Blue) and “Detected” (Black) n_FAM cutoff for clinical calling. Dashed lines represent the arithmetic mean of n_FAM. (D) Scatterplot n_FAM vs. ROX RFU. Solid lines represent the “Inconclusive” PLOD (Blue) and “Detected” (Black) n_FAM cutoff for clinical calling. Dotted line represents the 1600 RFU cutoff. Data are pooled from three 384-well arrays for both conditions. It is worth noting that in this experiment we observed a higher average ROX RFU for both the STARlet and manually-prepared MMIX. This was caused by a higher concentration of fluorophore in the manufacturer-supplied ROX reagent. However, the higher concentration of normalizing dye shifted the n_FAM values, thus the thresholds for just this pilot experiment were shifted accordingly to match the shift in the clusters. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

Journal: Slas Technology

Article Title: Development and validation of automated methods for COVID-19 PCR Master Mix preparation

doi: 10.1016/j.slast.2024.100195

Figure Lengend Snippet: STARlet-prepared MMIX compared with manually-prepared MMIX endpoint RFU data from PCR reactions using dilutions of synthetic RNA positive control material (Twist) at and around the limit of detection. (A) ROX RFU kernel distribution and RFU counts. Dotted line represents the 1600 ROX RFU cutoff. Dashed lines represent the arithmetic mean of ROX RFU. (B) Kernel Distribution and count for FAM RFU values. Dashed lines represent the arithmetic mean of FAM RFU. (C) Kernel Distribution and count for n_FAM values. Solid lines represent the “Inconclusive” PLOD (Blue) and “Detected” (Black) n_FAM cutoff for clinical calling. Dashed lines represent the arithmetic mean of n_FAM. (D) Scatterplot n_FAM vs. ROX RFU. Solid lines represent the “Inconclusive” PLOD (Blue) and “Detected” (Black) n_FAM cutoff for clinical calling. Dotted line represents the 1600 RFU cutoff. Data are pooled from three 384-well arrays for both conditions. It is worth noting that in this experiment we observed a higher average ROX RFU for both the STARlet and manually-prepared MMIX. This was caused by a higher concentration of fluorophore in the manufacturer-supplied ROX reagent. However, the higher concentration of normalizing dye shifted the n_FAM values, thus the thresholds for just this pilot experiment were shifted accordingly to match the shift in the clusters. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: Fig. 10 STARlet-prepared MMIX compared with manually-prepared MMIX endpoint RFU data from PCR reactions using dilutions of synthetic RNA positive control material (Seracare AccuPlex) at and around the limit of detection. (A) ROX RFU kernel distribution and RFU counts.

Techniques: Positive Control, Concentration Assay

STARlet-prepared MMIX compared with manually-prepared MMIX endpoint RFU data from PCR reactions using dilutions of synthetic RNA positive control material (Seracare AccuPlex) at and around the limit of detection. (A) ROX RFU kernel distribution and RFU counts. Dotted line represents the 1600 ROX RFU cutoff. Dashed lines represent the arithmetic mean of ROX RFU. (B) Kernel Distribution and count for FAM RFU values. Dashed lines represent the arithmetic mean of FAM RFU. (C) Kernel Distribution and count for n_FAM values. Solid lines represent the “Inconclusive” PLOD (Blue) and “Detected” (Black) n_FAM cutoff for clinical calling. Dashed lines represent the arithmetic mean of n_FAM (D) Scatterplot n_FAM vs. ROX RFU. Solid lines represent the “Inconclusive” PLOD (Blue) and “Detected” (Black) n_FAM cutoff for clinical calling. Dotted line represents the 1600 ROX RFU cutoff. Data are pooled from six 384-well arrays for both conditions. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

Journal: Slas Technology

Article Title: Development and validation of automated methods for COVID-19 PCR Master Mix preparation

doi: 10.1016/j.slast.2024.100195

Figure Lengend Snippet: STARlet-prepared MMIX compared with manually-prepared MMIX endpoint RFU data from PCR reactions using dilutions of synthetic RNA positive control material (Seracare AccuPlex) at and around the limit of detection. (A) ROX RFU kernel distribution and RFU counts. Dotted line represents the 1600 ROX RFU cutoff. Dashed lines represent the arithmetic mean of ROX RFU. (B) Kernel Distribution and count for FAM RFU values. Dashed lines represent the arithmetic mean of FAM RFU. (C) Kernel Distribution and count for n_FAM values. Solid lines represent the “Inconclusive” PLOD (Blue) and “Detected” (Black) n_FAM cutoff for clinical calling. Dashed lines represent the arithmetic mean of n_FAM (D) Scatterplot n_FAM vs. ROX RFU. Solid lines represent the “Inconclusive” PLOD (Blue) and “Detected” (Black) n_FAM cutoff for clinical calling. Dotted line represents the 1600 ROX RFU cutoff. Data are pooled from six 384-well arrays for both conditions. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: Fig. 10 STARlet-prepared MMIX compared with manually-prepared MMIX endpoint RFU data from PCR reactions using dilutions of synthetic RNA positive control material (Seracare AccuPlex) at and around the limit of detection. (A) ROX RFU kernel distribution and RFU counts.

Techniques: Positive Control