Journal: Aging Cell

Article Title: Extra virgin olive oil improves synaptic activity, short‐term plasticity, memory, and neuropathology in a tauopathy model

doi: 10.1111/acel.13076

Figure Lengend Snippet: Effect of chronic administration of EVOO‐rich diet on hippocampal pre‐ and postsynaptic protein expression in hTau mice. (a) Representative Western blot analyses for SNAP25, syntaxin 1A, synaptobrevin, complexin 1/2, and synaptophysin (SYP) in hippocampus homogenates from CTR and EVOO mice. (b) Densitometric analyses of the immunoreactivities to the antibodies shown in panel A (** p < .01; n = 6 per group) . (c) Representative Western blot analyses for the AMPAR subunit glutamate receptor 1 (GluR1), AMPAR subunit glutamate receptor 2 (GluR2), and their phosphorylated forms, pGluR1 (S831), pGluR1 (S845), and pGluR2 (S880), in hippocampus homogenates from CTR and EVOO mice. (d) Densitometric analyses of the immunoreactivities to the antibodies shown in panel A ( n = 6 per group) . Values are mean ± SEM

Article Snippet: Syntaxin 1A , AF7237 , RD SYSTEMS , Goat.

Techniques: Expressing, Western Blot

Journal: Aging Cell

Article Title: Extra virgin olive oil improves synaptic activity, short‐term plasticity, memory, and neuropathology in a tauopathy model

doi: 10.1111/acel.13076

Figure Lengend Snippet: Antibodies used in the study

Article Snippet: Syntaxin 1A , AF7237 , RD SYSTEMS , Goat.

Techniques:

Journal: Pathogens

Article Title: Cerebral Organoids Derived from a Parkinson’s Patient Exhibit Unique Pathogenesis from Chikungunya Virus Infection When Compared to a Non-Parkinson’s Patient

doi: 10.3390/pathogens10070913

Figure Lengend Snippet: Antibodies used for immunofluorescence studies.

Article Snippet: Syntaxin 1A , Mouse , Monoclonal , Novus Biologicals , 1:1000.

Techniques: Immunofluorescence

Journal: Journal of dermatological science

Article Title: Cathepsin K-upregulation in fibroblasts promotes matrigel invasive ability of squamous cell carcinoma cells via tumor-derived IL-1α.

doi: 10.1016/j.jdermsci.2010.09.005

Figure Lengend Snippet: Fig. 2. (A) Fbs were cultured with medium only, TGF-b1 (5 ng/ml), IL-8 (5 ng/ml), IL-1a (5 ng/ml), and IL-6 (5 ng/ml) for 24 h, and protein levels of CTSK were measured by Western blot. (B) Fbs were cultured with medium only, TGF-b1 (5 ng/ml), IL-8 (5 ng/ml), IL-1a (5 ng/ml), and IL-6 (5 ng/ml) for 24 h, and mRNA levels of CTSK were measured by real-time PCR. (C) Concentrations of IL-1a were assayed by ELISA in the supernatants of KC and SCC cells cultured for 24, 48 and 72 h. (D) The neutralizing anti-IL-1a antibody significantly reduced the upregulation of CTSK in Fbs induced by SCC in the coculture model.

Article Snippet: The sections were then incubated with antibodies against CTSK (mouse mAb, clone 3F9; 2 mg/ml; Biovendor, USA) and anti-human IL-1a antibody (1:50 dilution, R&D Systems) at 4 8C overnight.

Techniques: Cell Culture, Western Blot, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay

Journal: Journal of dermatological science

Article Title: Cathepsin K-upregulation in fibroblasts promotes matrigel invasive ability of squamous cell carcinoma cells via tumor-derived IL-1α.

doi: 10.1016/j.jdermsci.2010.09.005

Figure Lengend Snippet: Fig. 3. Immunohistochemical staining for CTSK (A) and IL-1a (B) in SCC. CTSK was strongly expressed in the majority of stromal Fbs in SCC. In parallel with the stromal CTSK expression, IL-1a expression was observed in the tumor cells of SCC (C).

Article Snippet: The sections were then incubated with antibodies against CTSK (mouse mAb, clone 3F9; 2 mg/ml; Biovendor, USA) and anti-human IL-1a antibody (1:50 dilution, R&D Systems) at 4 8C overnight.

Techniques: Immunohistochemical staining, Staining, Expressing

Journal: Journal of dermatological science

Article Title: Cathepsin K-upregulation in fibroblasts promotes matrigel invasive ability of squamous cell carcinoma cells via tumor-derived IL-1α.

doi: 10.1016/j.jdermsci.2010.09.005

Figure Lengend Snippet: Fig. 5. A431 SCC cells invaded matrigel in a time-dependent manner (^). Coculturing of Fb significantly upregulated the matrigel invasive ability of A431 SCC (~). However, the upregulation of invasive ability was significantly reduced when treated with neutralizing anti-IL-1a antibody (&). A431 SCC cells were counted excluding CD10 + Fbs (brown). *P < 0.05.

Article Snippet: The sections were then incubated with antibodies against CTSK (mouse mAb, clone 3F9; 2 mg/ml; Biovendor, USA) and anti-human IL-1a antibody (1:50 dilution, R&D Systems) at 4 8C overnight.

Techniques: