Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Neurological and immunological characteristics of a novel immortalized bovine brainstem-derived cell line and its susceptibility to arbovirus infection

doi: 10.3389/fcimb.2025.1518808

Figure Lengend Snippet: Establishment of the IKBM cell line. The morphology of the bovine brainstem medullary tissue-derived primary cells (A) or IKBM cells (B) established was examined under a phase-contrast microscope. The cumulative population doublings of IKBM cells were plotted against the duration of the culture period (in days) (C) . SV40LT protein expression was detected in the nuclei of IKBM cells [ brown , (D) ]. All nuclei were counterstained with hematoxylin [ blue , (D) ]. IKBM cells were cultured in the absence ( Control ) or presence of 100 μM forskolin ( Forskolin ) in 35-mm tissue culture dishes for 8 h (E) . Images are representative of at least two independent experiments.

Article Snippet: Cells were then incubated with a mouse monoclonal antibody against SV40LT (Oncogene Science, Cambridge, MA) at room temperature for 1 h in a humidified box.

Techniques: Derivative Assay, Microscopy, Expressing, Cell Culture, Control