Journal: Drug, Healthcare and Patient Safety

Article Title: Limitations of widely used high-risk human papillomavirus laboratory-developed testing in cervical cancer screening

doi: 10.2147/DHPS.S37273

Figure Lengend Snippet: Abnormal SurePath Pap test cells interpreted as “atypical squamous cells, cannot rule out a high-grade squamous intraepithelial lesion” (original magnification 400×); residual SurePath vial fluid tested negative using the Hybrid Capture 2 test for high-risk human papillomavirus. Note: Forty-two months later the patient was diagnosed with stage 1B1 cervical squamous cell carcinoma.

Article Snippet: HPV testing from the non-FDA-approved SurePath vial is thought to be more challenging, primarily due to the formaldehyde crosslinking of proteins and nucleic acids., Although recovery of DNA and ribonucleic acid is largely unaffected by long-term storage in PreservCyt, , storage in SurePath preservative fluid (Becton Dickinson) has been shown to affect the recovery of both DNA and ribonucleic acid., Upon exposure to SurePath media, recovery of both DNA and ribonucleic acid rapidly diminished.

Techniques:

Journal: Drug, Healthcare and Patient Safety

Article Title: Limitations of widely used high-risk human papillomavirus laboratory-developed testing in cervical cancer screening

doi: 10.2147/DHPS.S37273

Figure Lengend Snippet: Abnormal SurePath Pap test cells interpreted as “high-grade squamous intraepithelial lesion” (original magnification 400×); residual SurePath vial fluid tested negative using the Hybrid Capture 2 test for high risk human papillomavirus. Note: One month later the patient was diagnosed with stage 1B1 cervical squamous cell carcinoma.

Article Snippet: HPV testing from the non-FDA-approved SurePath vial is thought to be more challenging, primarily due to the formaldehyde crosslinking of proteins and nucleic acids., Although recovery of DNA and ribonucleic acid is largely unaffected by long-term storage in PreservCyt, , storage in SurePath preservative fluid (Becton Dickinson) has been shown to affect the recovery of both DNA and ribonucleic acid., Upon exposure to SurePath media, recovery of both DNA and ribonucleic acid rapidly diminished.

Techniques:

Journal: Drug, Healthcare and Patient Safety

Article Title: Limitations of widely used high-risk human papillomavirus laboratory-developed testing in cervical cancer screening

doi: 10.2147/DHPS.S37273

Figure Lengend Snippet: Invasive poorly differentiated cervical squamous carcinoma (original magnification 200×); diagnosed after two negative Hybrid Capture 2 human papillomavirus tests from SurePath vial fluid. Note: Human papillomavirus 16 DNA was detected in tumor tissue by polymerase chain reaction.

Article Snippet: HPV testing from the non-FDA-approved SurePath vial is thought to be more challenging, primarily due to the formaldehyde crosslinking of proteins and nucleic acids., Although recovery of DNA and ribonucleic acid is largely unaffected by long-term storage in PreservCyt, , storage in SurePath preservative fluid (Becton Dickinson) has been shown to affect the recovery of both DNA and ribonucleic acid., Upon exposure to SurePath media, recovery of both DNA and ribonucleic acid rapidly diminished.

Techniques: Polymerase Chain Reaction

Journal: Drug, Healthcare and Patient Safety

Article Title: Limitations of widely used high-risk human papillomavirus laboratory-developed testing in cervical cancer screening

doi: 10.2147/DHPS.S37273

Figure Lengend Snippet: Testing times allowed for of samples collected for Hybrid Capture 2 human papillomavirus testing as indicated on national laboratory websites

Article Snippet: HPV testing from the non-FDA-approved SurePath vial is thought to be more challenging, primarily due to the formaldehyde crosslinking of proteins and nucleic acids., Although recovery of DNA and ribonucleic acid is largely unaffected by long-term storage in PreservCyt, , storage in SurePath preservative fluid (Becton Dickinson) has been shown to affect the recovery of both DNA and ribonucleic acid., Upon exposure to SurePath media, recovery of both DNA and ribonucleic acid rapidly diminished.

Techniques: