Journal: Molecular Metabolism

Article Title: Differential cell type-specific function of the aryl hydrocarbon receptor and its repressor in diet-induced obesity and fibrosis

doi: 10.1016/j.molmet.2024.101963

Figure Lengend Snippet: AhRR-deficient mice are partially resistant to diet-induced obesity. ( A ) Body weight of wild-type (WT) and AhRR-deficient mice fed CD or HFD for 14 weeks; n = 8 (CD WT), n = 6 (HFD WT), n = 5 (CD, HFD AhRR −/− ). ( B ) Blood glucose levels during a glucose tolerance test -GTT- (left panel) and insulin tolerance test -ITT- (right panel) after 12 weeks on HFD; n = 11 (GTT WT), n = 10 (GTT AhRR −/− ), n = 5 (ITT WT, ITT AhRR −/− ). ( C ) Total serum cholesterol levels after 14 weeks on HFD; n = 9 (WT), n = 7 (AhRR −/− ). ( D ) Analysis of covariance (ANCOVA) (non-linear fit) of oxygen consumption/body weight (BW); n = 8 for both groups. ( E ) Total body fat mass assessed by NMR; n = 8 for both groups. ( F ) WATg weight; n = 8 for both groups. ( G ) Representative H&E staining. Scale bars, 80 μm (left panel). Right, quantification of adipocyte size of the WATg of HFD mice; n = 5 (WT), n = 4 (AhRR −/− ). ( H ) Liver weight; n = 8 (CD WT, CD AhRR −/− , HFD AhRR −/− ), n = 7 (HFD WT). ( I ) Representative H&E (left panel) and Sirius Red (right panel) staining of liver sections. Scale bars, 100 μm. ( J ) Representative Oil Red O (ORO) and Hemalaun staining of liver sections of WT and AhRR-deficient mice after HFD. Scale bars, 80 μm (left panel). Right, quantification of ORO stain, n = 3 (CD WT), n = 8 (HFD WT), n = 6 (HFD AhRR −/− ). ( K ) Serum Alanine transaminase (ALT) concentration of WT and AhRR-deficient mice fed either CD or HFD for 14 weeks; n = 4 for all groups. ( L ) Body weight of WT and AhRR-deficient mice fed a HFD supplemented with or without indole-3 carbinole (I3C) for 14 weeks; n = 20 (WT HFD), n = 15 (WT HFD + I3C), n = 15 (AhRR −/− HFD), and n = 14 (AhRR −/− HFD + I3C). ( M ) Representative ORO and Hemalaun staining of liver sections of WT and AhRR −/− mice after HFD ± I3C. Scale bars, 100 μm (left panel). Right, ORO staining quantification of liver sections; n = 10 (WT CD, HFD), n = 7 (WT HFD + I3C), n = 5 (AhRR −/− HFD), n = 9 (AhRR −/− HFD + I3C). ( N ) z-scored intensity values of representative TCA-metabolites found in plasma of mice after 14 weeks of CD or HFD ± I3C; n = 3–5. ∗p < 0.05, ∗∗p ≤ 0.01, ∗∗∗p ≤ 0.001. Significance was determined using unpaired two-tailed t-tests (A, B, C, G) and one-way analysis of variance (ANOVA) with Tukey's multiple-comparison test (E, F, H, K and M) or with Dunnett's multiple comparisons test (J). Two-way analysis of variance (ANOVA) with Tukey's multiple-comparison test (L). Data are mean ± s.e.m.

Article Snippet: For dietary intervention studies, mice were exposed to a purified diet or with 2 g/kg indole-3-carbinole (I3C)-supplemented control diet (CD/CD+I3C, 13 kJ% Fat, Cat No.: D12450 LS ssniff EF) or a purified or with 2 g/kg I3C-supplemented high fat diet (HFD/HFD+I3C, 60 kJ% fat, Cat. No.: D12492 ssniff EF) ad libitum .

Techniques: Staining, Concentration Assay, Two Tailed Test, Comparison