Journal: PLoS ONE

Article Title: Metabolism of AGEs – Bacterial AGEs Are Degraded by Metallo-Proteases

doi: 10.1371/journal.pone.0074970

Figure Lengend Snippet: Lysate was extracted from exponentially growing cultures and further separated into high and low-molecular-mass fractions, as described in Materials and Methods. The high molecular weight fraction was treated and incubated at 37°C for 3 hours and then separated by size using HiLoad 16/600 Superdex 75 pg 120 ml filtration column. After 30 ml of elution, 4 ml fractions were collected and AGEs specific fluorescence was determined. The graph represents the size distribution of AGEs in an untreated sample, lystae treated with 3M urea and lystae treated with protease inhibitor cocktail (Sigma).

Article Snippet: For AGEs size exclusion filtration measurements ( ) samples were separated using GE HiLoad 16/600 Superdex 75 pg 120ml filtration column, equilibrated with PBS.

Techniques: Molecular Weight, Incubation, Filtration, Fluorescence, Protease Inhibitor

Journal: PLoS ONE

Article Title: Metabolism of AGEs – Bacterial AGEs Are Degraded by Metallo-Proteases

doi: 10.1371/journal.pone.0074970

Figure Lengend Snippet: Effect of 100 µM 1,10 phenantholine (Sigma) on AGEs degradation. Bacteria were grown as described in materials and methods. Lysate was extracted from treated and untreated cells and further separated by size using HiLoad 16/600 Superdex 75 pg 120 ml filtration column. After 30 ml of elution, 4 ml fractions were collected and AGEs specific fluorescence was determined.

Article Snippet: For AGEs size exclusion filtration measurements ( ) samples were separated using GE HiLoad 16/600 Superdex 75 pg 120ml filtration column, equilibrated with PBS.

Techniques: Filtration, Fluorescence

Journal: Cell

Article Title: Structure of the MIS12 Complex and Molecular Basis of Its Interaction with CENP-C at Human Kinetochores

doi: 10.1016/j.cell.2016.10.005

Figure Lengend Snippet:

Article Snippet: Superdex 200 Increase 5/150 GL , GE Healthcare , Cat#28-9909-45.

Techniques: Generated, Recombinant, Protease Inhibitor, Plasmid Preparation, Software, Western Blot