Journal: Methods in molecular biology (Clifton, N.J.)

Article Title: Discovery of RNA Binding Small Molecules Using Small Molecule Microarrays

doi: 10.1007/978-1-4939-6584-7_11

Figure Lengend Snippet: Overview of SMM strategy to identify RNA-binding small molecules. Small molecule libraries prepared in 384-well plates are utilized to print onto isocyanate-functionalized slides by the microarrayer. Prepared SMMs are next incubated with the labeled RNA of interest and scanned to detect hits

Article Snippet: Printing of Small Molecule Microarrays MicroGrid II Microarrayer (BioRobotics) or equivalent.

Techniques: RNA Binding Assay, Incubation, Labeling

Journal: Acta biomaterialia

Article Title: Development of peptide-functionalized synthetic hydrogel microarrays for stem cell and tissue engineering applications

doi: 10.1016/j.actbio.2016.09.006

Figure Lengend Snippet: Schematic representation of fabrication of peptide-functionalized PEG hydrogel microarrays. A. The printing solutions composed of PEGDA monomer and various methacrylated peptides were prepared in a 384-well plate. B. The printing solutions were placed onto poly(HEMA) coated microscope slides with a customized microarrayer and polymerized by UV under Argon protection to prepare peptide-functionalized PEG hydrogel spots. Eight hydrogel spots in a microarray were shown to present the dimension of the hydrogel spots and the distance between the hydrogel spots. C. High throughput analysis of cellular activities after cell seeding onto the microarray.

Article Snippet: The microarrays were printed in a humid Ar-atmosphere on epoxy monolayer-coated glass slides (Xenopore XENOSLIDE E, Hawthorne, NJ) that were first dip-coated in 4 v/v% poly(hydroxyethyl methacrylate) (i.e., poly(HEMA)) using a customized microarrayer (Biodot).

Techniques: Microscopy, Microarray, High Throughput Screening Assay