Journal: Journal of Lipid Research

Article Title: Selective desorption/ionization of sulfatides by MALDI-MS facilitated using 9-aminoacridine as matrix

doi: 10.1194/jlr.D004077

Figure Lengend Snippet: Mass spectral comparison of sulfatide molecular species present in mouse brain cortical lipid extracts acquired by either MALDI-MS or ESI-MS. Lipid extracts of mouse brain cortices were prepared by a modified Bligh and Dyer procedure as described in “Materials and Methods.” A: A negative-ion mass spectrum of a crude lipid extract of mouse brain cortex acquired by MALDI-MS utilizing 9-AA as matrix dissolved in methanol-water solution (90:10, v/v). B: A negative-ion mass spectrum of an alkaline-treated lipid extract of mouse brain cortex acquired by ESI-MS as described in “Materials and Methods.” C: A negative-ion mass spectrum of a crude lipid extract of mouse brain cortex acquired by MALDI-MS utilizing DHB acid as matrix dissolved in water. IS, Internal standard.

Article Snippet: N-Palmitoyl sulfatide (N16:0 sulfatide) was obtained from Matreya LLC (Pleasant Gap, PA).

Techniques: Modification

Journal: Journal of Lipid Research

Article Title: Selective desorption/ionization of sulfatides by MALDI-MS facilitated using 9-aminoacridine as matrix

doi: 10.1194/jlr.D004077

Figure Lengend Snippet: Determination of enhanced desorption/ionization of sulfatides over PI species by MALDI-MS using 9-AA as matrix. Lipid mixtures comprised of N16:0 sulfatide (0.2 pmol/μl) and soy PIs [either 1 pmol/μl (A) or 10 pmol/μl (B)] were prepared and analyzed by MALDI-MS using 9-AA as matrix as described in “Materials and Methods.”

Article Snippet: N-Palmitoyl sulfatide (N16:0 sulfatide) was obtained from Matreya LLC (Pleasant Gap, PA).

Techniques:

Journal: Journal of Lipid Research

Article Title: Selective desorption/ionization of sulfatides by MALDI-MS facilitated using 9-aminoacridine as matrix

doi: 10.1194/jlr.D004077

Figure Lengend Snippet: Product-ion analyses of sulfatide species in the negative ion refl ector mode using 9-AA as matrix. Tandem mass spectra of d18:1-N24:1 sulfatide at m/z 888.67 (A) and d18:1-[OH]N24:0 sulfatide at m/z 906.67 (B) present in mouse brain cortical lipid extracts acquired by MALDI-TOF/TOF-MS in the negative ion mode as described in “Materials and Methods.” Each tandem mass spectrum was recorded on a 4800 MALDI-TOF/TOF Analyzer in the negative ion refl ector mode using 9-AA as matrix using collision-induced dissociation with the metastable suppressor on and the timed ion selector enabled. The voltages of source 1, collision cell, and collision cell offset were 8.0, 7.0, and −0.035 kV, respectively. The tandem mass spectrum was obtained by averaging 3000 consecutive laser shots (50 shots/subspectra with 60 total subspectra).

Article Snippet: N-Palmitoyl sulfatide (N16:0 sulfatide) was obtained from Matreya LLC (Pleasant Gap, PA).

Techniques:

Journal: Journal of Lipid Research

Article Title: Selective desorption/ionization of sulfatides by MALDI-MS facilitated using 9-aminoacridine as matrix

doi: 10.1194/jlr.D004077

Figure Lengend Snippet: Determination of the limit of detection or quantitation of MALDI-MS analysis of sulfatides using 9-AA as matrix. A mixture of N12:0, N16:0, and bovine brain sulfatides at a molar ratio of 1:1:2 was prepared and MALDI-MS analyses of this mixture after dilution were performed as described in “Materials and Methods.” A: A negative-ion mass spectrum of the mixture at a concentration of 200 fmol/μl. B: A negative-ion mass spectrum of the mixture at a concentration of 4 fmol/μl. C: The linear relationship of the ratios of ion peak intensities of representative bovine brain sulfatide species and each of the selected internal standards with different concentrations of the mixture. The data points represent the mean ± SD from four mixture preparations at each concentration. Note that some error bars are within the symbols. IS, Internal standard.

Article Snippet: N-Palmitoyl sulfatide (N16:0 sulfatide) was obtained from Matreya LLC (Pleasant Gap, PA).

Techniques: Quantitation Assay, Concentration Assay

Journal: Journal of Lipid Research

Article Title: Selective desorption/ionization of sulfatides by MALDI-MS facilitated using 9-aminoacridine as matrix

doi: 10.1194/jlr.D004077

Figure Lengend Snippet: Quantitative analyses of sulfatide species by MALDI-MS using 9-AA as matrix. Mixtures comprised of equimolar N12:0 and N16:0 sulfatides as internal standards and different amounts of bovine brain sulfatides were prepared and MALDI-MS analyses of the mixtures after proper dilution were performed as described in “Materials and Methods.” A, B: The negative-ion mass spectra of mixtures at the concentration of 5 and 250 fmol/μl, respectively. C: The linear log correlation between molar ratios and ion peak intensity ratios of representative bovine brain sulfatide species versus both of the selected internal standards. The data points represent the mean ± SD from four mixture preparations of each molar ratio of bovine brain sulfatides to the selected internal standards. Note that some error bars are within the symbols. IS, Internal standard; ST, sulfatide.

Article Snippet: N-Palmitoyl sulfatide (N16:0 sulfatide) was obtained from Matreya LLC (Pleasant Gap, PA).

Techniques: Concentration Assay

Journal: Journal of Lipid Research

Article Title: Selective desorption/ionization of sulfatides by MALDI-MS facilitated using 9-aminoacridine as matrix

doi: 10.1194/jlr.D004077

Figure Lengend Snippet: MALDI-MS mass spectra of sulfatides in lipid extracts of mouse spinal cord, kidney medulla, kidney cortex, and skeletal muscle. Total lipids of spinal cord, kidney medulla, kidney cortex, and skeletal muscle of mice at 4–6 months of age were extracted by using a modifi ed procedure of Bligh and Dyer against 50 mM LiCl as previously described (39). Part of the lipid extract of mouse kidney medulla, kidney cortex, or skeletal muscle was treated with lithium methoxide as previously described (27). MALDI mass spectra of lipid extracts of mouse spinal cord (A), kidney medulla (B), kidney cortex (C), and skeletal muscle (D) were acquired by averaging 3,000 consecutive laser shots (50 shots/subspectra with 60 total subspectra) using the 4800 MALDI TOF/TOF Analyzer with 9-AA as matrix. IS, Internal standard; ST, sulfatide.

Article Snippet: N-Palmitoyl sulfatide (N16:0 sulfatide) was obtained from Matreya LLC (Pleasant Gap, PA).

Techniques:

Journal: Journal of Lipid Research

Article Title: Selective desorption/ionization of sulfatides by MALDI-MS facilitated using 9-aminoacridine as matrix

doi: 10.1194/jlr.D004077

Figure Lengend Snippet: Quantitative analysis of sulfatide species in the lipid extracts from a variety of mouse tissue samples and plasma a

Article Snippet: N-Palmitoyl sulfatide (N16:0 sulfatide) was obtained from Matreya LLC (Pleasant Gap, PA).

Techniques: