Journal: European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences

Article Title: HLM chip - A microfluidic approach to study the mechanistic basis of cytochrome P450 inhibition using immobilized human liver microsomes.

doi: 10.1016/j.ejps.2024.106773

Figure Lengend Snippet: Figure 5. The inhibitory impacts of increasing concentration gradients of (A) sulfaphenazole and (B)

Article Snippet: In the CYP2C9 inhibition assays, Luciferin-H concentration was kept constant (200 μM) over the entire experiment, whereas seven-step concentration gradients of the CYP2C9 inhibitors, sulfaphenazole (0.05-20 μM, reversible, Toronto Research Chemicals, Toronto, ON, Canada) and tienilic acid (0-200 μM, irreversible, Sigma Aldrich, St. Louis, MO), as well as the nonselective CYP inhibitor, miconazole (0-50 μM, Sigma Aldrich, St. Louis, MO), were established, each individually, in the same manner as in enzyme kinetic experiments.

Techniques: Concentration Assay

Journal: American Journal of Translational Research

Article Title: Protective effect of pantoprazole against sepsis-induced acute lung and kidney injury in rats

doi:

Figure Lengend Snippet: Pantoprazole reduces the expression of A(SPA) and D(SPD) in lung injury. (A) Western blot to show the protein levels of A(SPA) and D(SPD) in control, model, sham and Pantoprazole-treatment groups. Quantification assay of the A(SPA) and D(SPD) band’s intensity. Error bars indicate ± SD. **P < 0.01 vs. Control group. **P < 0.01, ***P < 0.001 vs. Control group; ###P < 0.001 vs. model group and ΔΔP < 0.01, ΔΔΔP < 0.001 vs. sham group. The expression of A(SPA) (B) and D(SPD) (C) in lung injury tissues were assessed by IHC analysis (Original magnification).

Article Snippet: The antibodies used were as follows: A(SPA) (1:1,000, Santa Cruz), D(SPD) (1:1,000, Santa Cruz), RHOA (1:1,000, Santa Cruz), ROCK1 (1:1,000, Santa Cruz), ROCK2 (1:1,000, Millipore), LIMK1 (1:1,000, Abcam), LIMK2 (1:1,000, Abcam), mouse anti-rabbit secondary antibody (1:10000, Santa Cruz -2077), rabbit anti-mouse secondary antibody (1:10000, Santa Cruz -2061).

Techniques: Expressing, Western Blot

Journal: Xenobiotica; the fate of foreign compounds in biological systems

Article Title: In vitro metabolism of piperaquine is primarily mediated by CYP3A4

doi: 10.3109/00498254.2012.693972

Figure Lengend Snippet: The effect of isoform selective P450 inhibitors on piperaquine metabolism. The data is expressed as percent piperaquine remaining (mean ± SD of triplicate determinations) as a function of time following human liver microsome incubations in the presence of isoform selective P450 inhibitors. The starting concentration of piperaquine was 0.6 µM. The following concentrations of inhibitors were used: 1 µM ketoconazole (CYP3A4), 5 µM ticlopidine (CYP2C19), 20 µM furafylline (CYP1A2), 20 µM sulfaphenazole (2C9), and 25 µM quercetin (CYP2C8).

Article Snippet: Sulfaphenazole and furafylline were purchased from BD Biosciences (San Jose, CA).

Techniques: Concentration Assay

Journal: Cell

Article Title: Discovery of natural-product-derived sequanamycins as potent oral anti-tuberculosis agents

doi: 10.1016/j.cell.2023.01.043

Figure Lengend Snippet:

Article Snippet: Ketoconazole , BD Gentest , Cat#451023.

Techniques: Mutagenesis, Recombinant, Cell Culture, Methylation, Amplification, Software, Microscopy, RNA Extraction, Real-time Polymerase Chain Reaction