subsp Search Results


salmonella enterica enteritidis pt 30  (ATCC)
95
ATCC salmonella enterica enteritidis pt 30
Salmonella Enterica Enteritidis Pt 30, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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s aureus atcc 43300  (ATCC)
99
ATCC s aureus atcc 43300
S Aureus Atcc 43300, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nucleatum  (ATCC)
99
ATCC nucleatum
Nucleatum, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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s aureus atcc 29213  (ATCC)
99
ATCC s aureus atcc 29213
S Aureus Atcc 29213, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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s aureus atcc 25923  (ATCC)
99
ATCC s aureus atcc 25923
S Aureus Atcc 25923, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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e cloacae type strain atcc 13047  (ATCC)
99
ATCC e cloacae type strain atcc 13047
E Cloacae Type Strain Atcc 13047, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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l pneumophila serogroup 1 atcc 33152  (ATCC)
97
ATCC l pneumophila serogroup 1 atcc 33152
L Pneumophila Serogroup 1 Atcc 33152, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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m tuberculosis h37rv  (ATCC)
99
ATCC m tuberculosis h37rv
M Tuberculosis H37rv, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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atcc 13076  (ATCC)
99
ATCC atcc 13076
Atcc 13076, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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c m subsp sepedonicus atcc 33113  (ATCC)
92
ATCC c m subsp sepedonicus atcc 33113
(A) and ( B) . TaqMan real-time qPCR assays with individual primer set harboring no complementary 5’ AT-rich sequences. ( A) represents the detection C. michiganens is; and (B) represents the subspecific detection of C. m. subsp. nebraskensis ; ( C, D, E, F and G ) are multiplex and single reactions after adding the non-complementary 5’ AT-rich sequences. (C) and (D) multiplex reactions for simultaneous detection of all subspecies of C. michiganens is and of C. m. subsp. nebraskensis , respectively. (E) and (F) multiplex reactions conducted for simultaneous detection of C. michiganens is subspecies and of C. m. subsp. nebraskensis , respectively, after adding 1 μl of healthy plant corn DNA to each 10-fold serially diluted sensitivity reactions in order to simulate natural infection. (G) and (H) TaqMan real-time qPCR assays performed with individual primer and probe sets to detect C. michiganens is subspecies and C. m. subsp. nebraskensis , respectively. All assays were conducted from 10 ng to 10 fg serially diluted genomic DNA of C. m. subsp. nebraskensis (strain A6206) using Rotorgene.
C M Subsp Sepedonicus Atcc 33113, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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staphylococcus hominis  (ATCC)
95
ATCC staphylococcus hominis
(A) and ( B) . TaqMan real-time qPCR assays with individual primer set harboring no complementary 5’ AT-rich sequences. ( A) represents the detection C. michiganens is; and (B) represents the subspecific detection of C. m. subsp. nebraskensis ; ( C, D, E, F and G ) are multiplex and single reactions after adding the non-complementary 5’ AT-rich sequences. (C) and (D) multiplex reactions for simultaneous detection of all subspecies of C. michiganens is and of C. m. subsp. nebraskensis , respectively. (E) and (F) multiplex reactions conducted for simultaneous detection of C. michiganens is subspecies and of C. m. subsp. nebraskensis , respectively, after adding 1 μl of healthy plant corn DNA to each 10-fold serially diluted sensitivity reactions in order to simulate natural infection. (G) and (H) TaqMan real-time qPCR assays performed with individual primer and probe sets to detect C. michiganens is subspecies and C. m. subsp. nebraskensis , respectively. All assays were conducted from 10 ng to 10 fg serially diluted genomic DNA of C. m. subsp. nebraskensis (strain A6206) using Rotorgene.
Staphylococcus Hominis, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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kgy obtenues lors de notre étude pour salmonella typhimurium atcc 14028 sont  (ATCC)
99
ATCC kgy obtenues lors de notre étude pour salmonella typhimurium atcc 14028 sont
(A) and ( B) . TaqMan real-time qPCR assays with individual primer set harboring no complementary 5’ AT-rich sequences. ( A) represents the detection C. michiganens is; and (B) represents the subspecific detection of C. m. subsp. nebraskensis ; ( C, D, E, F and G ) are multiplex and single reactions after adding the non-complementary 5’ AT-rich sequences. (C) and (D) multiplex reactions for simultaneous detection of all subspecies of C. michiganens is and of C. m. subsp. nebraskensis , respectively. (E) and (F) multiplex reactions conducted for simultaneous detection of C. michiganens is subspecies and of C. m. subsp. nebraskensis , respectively, after adding 1 μl of healthy plant corn DNA to each 10-fold serially diluted sensitivity reactions in order to simulate natural infection. (G) and (H) TaqMan real-time qPCR assays performed with individual primer and probe sets to detect C. michiganens is subspecies and C. m. subsp. nebraskensis , respectively. All assays were conducted from 10 ng to 10 fg serially diluted genomic DNA of C. m. subsp. nebraskensis (strain A6206) using Rotorgene.
Kgy Obtenues Lors De Notre étude Pour Salmonella Typhimurium Atcc 14028 Sont, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


(A) and ( B) . TaqMan real-time qPCR assays with individual primer set harboring no complementary 5’ AT-rich sequences. ( A) represents the detection C. michiganens is; and (B) represents the subspecific detection of C. m. subsp. nebraskensis ; ( C, D, E, F and G ) are multiplex and single reactions after adding the non-complementary 5’ AT-rich sequences. (C) and (D) multiplex reactions for simultaneous detection of all subspecies of C. michiganens is and of C. m. subsp. nebraskensis , respectively. (E) and (F) multiplex reactions conducted for simultaneous detection of C. michiganens is subspecies and of C. m. subsp. nebraskensis , respectively, after adding 1 μl of healthy plant corn DNA to each 10-fold serially diluted sensitivity reactions in order to simulate natural infection. (G) and (H) TaqMan real-time qPCR assays performed with individual primer and probe sets to detect C. michiganens is subspecies and C. m. subsp. nebraskensis , respectively. All assays were conducted from 10 ng to 10 fg serially diluted genomic DNA of C. m. subsp. nebraskensis (strain A6206) using Rotorgene.

Journal: bioRxiv

Article Title: Synergetic effect of non-complementary 5’ AT-rich sequences on the development of a multiplex TaqMan real-time PCR for specific and robust detection of Clavibacter michiganensis and C. michiganensis subsp. nebraskensis

doi: 10.1101/566281

Figure Lengend Snippet: (A) and ( B) . TaqMan real-time qPCR assays with individual primer set harboring no complementary 5’ AT-rich sequences. ( A) represents the detection C. michiganens is; and (B) represents the subspecific detection of C. m. subsp. nebraskensis ; ( C, D, E, F and G ) are multiplex and single reactions after adding the non-complementary 5’ AT-rich sequences. (C) and (D) multiplex reactions for simultaneous detection of all subspecies of C. michiganens is and of C. m. subsp. nebraskensis , respectively. (E) and (F) multiplex reactions conducted for simultaneous detection of C. michiganens is subspecies and of C. m. subsp. nebraskensis , respectively, after adding 1 μl of healthy plant corn DNA to each 10-fold serially diluted sensitivity reactions in order to simulate natural infection. (G) and (H) TaqMan real-time qPCR assays performed with individual primer and probe sets to detect C. michiganens is subspecies and C. m. subsp. nebraskensis , respectively. All assays were conducted from 10 ng to 10 fg serially diluted genomic DNA of C. m. subsp. nebraskensis (strain A6206) using Rotorgene.

Article Snippet: To calculate the core genome in the C. michiganensis subspecies and the variable genomes for each subspecies, the annotated genomes of gram-positive bacteria, C. m. subsp. nebraskensis NCPPC 2581 (NC_020891), C. m. subsp. michiganensis NCPPB 382 (NC_009480), C. m. subsp. sepedonicus ATCC 33113 (NC_010407), C. m. subsp. insidiosus strain R1-1 (NZ_CP011043), and C. m. subsp. capsici strain PF008 (NZ_CP012573) were used.

Techniques: Multiplex Assay, Infection