Journal: Data in Brief

Article Title: Data on cardiac lncRNA STX18-AS1 expression in developing human hearts and function during in vitro hESC-cardiomyocyte differentiation

doi: 10.1016/j.dib.2022.108770

Figure Lengend Snippet: Genomic location and conservation scores of STX18-AS1 and surrounding genes between Human and Mouse genome. STX18 and MSX1 are the two neighbouring genes of STX18-AS1 in humans. No annotated genes at a similar location between STX18 and MSX1 are identified in the mouse genome. Phylop score shows the conservation score spare species. The sequences across STX18-AS1 have lower Phylop scores than neighbouring genes.

Article Snippet: STX18 , Gene expression , Hs00560288_g1.

Techniques:

Journal: Data in Brief

Article Title: Data on cardiac lncRNA STX18-AS1 expression in developing human hearts and function during in vitro hESC-cardiomyocyte differentiation

doi: 10.1016/j.dib.2022.108770

Figure Lengend Snippet: Risk SNPs for ASD and eQTL analyses between SNPs and STX18-AS1 . a, Linkage disequilibrium (LD) across STX18-AS1 with SNP data from CEU population of 1000 Genomes Project. The LD map was generated with HaploView. The LD of SNP pairs is represented as r2 in white to red (0-1). The locations of the three risk SNPs identified in GWAS are labelled in red bars at the top of the figure. The black triangles represent haplotype blocks (defining with confidence intervals according to Gabriel's method). b-d, eQTL analyses with 108 human heart right atrial appendage (RAA) samples using the qPCR method for three top SNPs identified from GWAS, rs870142 (b), rs16835979(c), and rs6824295(d). The significant association between SNPs and STX18-AS1 transcription was tested using the linear regression model, with P values between 0.038-0.039. e-f, The eQTL analyses of rs870142 and STX18-AS1 transcription in internal mammary artery (IMA) samples (e) and human blood samples (f). The linear regressions are not significant for both IMA and blood eQTL analyses.

Article Snippet: STX18 , Gene expression , Hs00560288_g1.

Techniques: Generated

Journal: Data in Brief

Article Title: Data on cardiac lncRNA STX18-AS1 expression in developing human hearts and function during in vitro hESC-cardiomyocyte differentiation

doi: 10.1016/j.dib.2022.108770

Figure Lengend Snippet: STX18-AS1 expression in human tissues and developing hearts. a, comparison of STX18-AS1 transcription in Adult and Foetal tissues (cDNA from Human MTCTM Panel I and Human Foetal MTCTM Panel). b-g, whole-mount ISH of human embryonic hearts (b-c, CS17; d-e, CS18; f-g, CS19) from ventral and dorsal views. Blue colour indicates the STX18-AS1 expression in hearts. h-j, the section of all three ISH hearts (h, CS17 heart; i, CS18 heart; j, CS19 heart) with cuts at the view of OFT. k-m, section of all three ISH hearts (k, CS17 heart; l, CS18 heart; m, CS19 heart) with cuts at the view of AS. RA, right atrium; LA, left atrium; RV, right ventricle; LV, left ventricle; OFT, out-flow tract; AS, atrial septum (yellow); AO, aorta; PA, pulmonary artery; VS, ventricular septum. STX18-AS1 transcripts are stained blue and counterstained with Eosin.; scale bars are 200µm.

Article Snippet: STX18 , Gene expression , Hs00560288_g1.

Techniques: Expressing, Comparison, Staining

Journal: Data in Brief

Article Title: Data on cardiac lncRNA STX18-AS1 expression in developing human hearts and function during in vitro hESC-cardiomyocyte differentiation

doi: 10.1016/j.dib.2022.108770

Figure Lengend Snippet: STX18-AS1 haploinsufficiency restricted in vitro hESC-cardiomyocyte differentiation. a, Immunostaining of cTNT (red fluorescence, referencing to DAPI in blue) at Day 8 of differentiation in both Control CRISPR hESC-CMs and STX18-AS1 CRISPR cell pool (KD in following gragh) derived CMs. Scale bars represent 200µm. b-c, the quantitative analyses of cTNT immunostaining on fluorescence density (b) and cTNT-positive cells (c). **, P < 0.01, using t -test. d, Time-course expression of STX18-AS1 during the CM differentiation from Control CRISPR and STX18-AS1 CRISPR cell pool. e-k, the time-course transcription changes of neighbouring genes [ MSX1 (e) and STX18 (f)], pluripotency marker [ SOX2 (g)], and key cardiac transcriptional factors [ NKX2-5 (h), ISL1 (i), GATA4 (j), and TBX5 (k)] during D0-D15 of hESC-CM differentiation. *, P < 0.05. Two-way ANOVA test with Bonferroni adjustment is applied for generating the P values for comparisons at each time point ( n >=3).

Article Snippet: STX18 , Gene expression , Hs00560288_g1.

Techniques: In Vitro, Immunostaining, Fluorescence, Control, CRISPR, Derivative Assay, Expressing, Marker

Journal: Data in Brief

Article Title: Data on cardiac lncRNA STX18-AS1 expression in developing human hearts and function during in vitro hESC-cardiomyocyte differentiation

doi: 10.1016/j.dib.2022.108770

Figure Lengend Snippet: TaqMan assay probes for qPCR.

Article Snippet: STX18 , Gene expression , Hs00560288_g1.

Techniques: TaqMan Assay, Gene Expression

Journal: Data in Brief

Article Title: Data on cardiac lncRNA STX18-AS1 expression in developing human hearts and function during in vitro hESC-cardiomyocyte differentiation

doi: 10.1016/j.dib.2022.108770

Figure Lengend Snippet:

Article Snippet: STX18 , Gene expression , Hs00560288_g1.

Techniques: Functional Assay, Microscopy, Derivative Assay, In Vitro