Journal: PLoS ONE

Article Title: The Prion Protein Controls Polysialylation of Neural Cell Adhesion Molecule 1 during Cellular Morphogenesis

doi: 10.1371/journal.pone.0133741

Figure Lengend Snippet: (a) PrP is not required for NCAM1 polysialylation in N2a cells made to express ST8SIA2 from a heterologous expression plasmid. Note that higher levels of PSA-NCAM1 in CRISPR-Cas9-based PrP knockout N2a cells reflect higher levels of NCAM1 substrate in the PrP ko cell clones. (b) CRISPR-Cas9-based knockout of PrP in muscle C2C12 myocytes has little effect on total NCAM1 levels but causes profound upregulation of NCAM1 polysialylation before or throughout myotube differentiation. The control represents a C2C12 cell clone, which underwent all steps of CRISPR-Cas9 manipulation as the positive PrP ko clone but did not give rise to PrP ablation. FBS, cells grown in fetal bovine serum; HS, cells grown in horse serum (known to induce myotube formation). (c) Stable PrP ko or kd, but not transient PrP kd, impairs upregulation of ST8SIA2 transcripts in NMuMG cells in response to TGFB1 exposure. Note the different ordinate scales of subpanels. (d) ST8SIA2 is the polyST primarily responsible for EMT-dependent NCAM1 polysialylation in the NMuMG cell model. Transient kd of ST8SIA2 in NMuMG cells mimics stable PrP-deficiency with regard to its inhibition of NCAM1 polysialylation. Note that in contrast to the stable PrP knockout or kd, transient PrP-kd does not interfere with NCAM1 polysialylation.

Article Snippet: The plasmids coding for ST8SIA2 (MR205823) and ST8SIA4 (MR205502) were purchased from Origene (MD, USA).

Techniques: Expressing, Plasmid Preparation, CRISPR, Knock-Out, Clone Assay, Inhibition