Journal: Clinical cancer research : an official journal of the American Association for Cancer Research

Article Title: Anastrozole has an association between degree of estrogen suppression and outcomes in early breast cancer and is a ligand for estrogen receptor α

doi: 10.1158/1078-0432.CCR-19-3091

Figure Lengend Snippet: Anastrozole potentiates estrogen effects when estrogen levels are above the thresholds. A. Cell proliferation of MCF7 cells in the presence of anastrozole (A) (27 ng/mL) plus indicated concentrations of estrone (E1) (0.13, 1.3, 2.6, and 26 pg/mL) and estradiol (E2) (0.1, 0.5, 2.5, and 25 pg/mL). B. Proliferation of MCF7 cells in the presence of letrozole (L) (215 ng/mL) plus indicated concentrations of estrone (E1) and estradiol (E2). C. Cell proliferation of MCF7 cells in the presence of exemestane (E) (10 ng/mL) plus E1 and E2. D-F. Estrogen response element dependent luciferase assay in MCF7 cells treated with the indicated concentrations of E1 (0.13, 1.3, 2.6, and 26 pg/mL), E2 (0.1, 0.5, 2.5, and 25 pg/mL) alone or in combination with anastrozole (A) (27 ng/mL), letrozole (L) (215 ng/mL), and exemestane (E) (10 ng/mL). Error bars represent the SEM of three independent experiments. **p<0.01.

Article Snippet: Laboratory Studies Cell lines Human ER+ breast cancer cell lines T47D and MCF7 were obtained from American Type Culture Collection (ATCC, Manassus, VA) in 2014 and the identities of all cell lines were confirmed by the medical genome facility at Mayo Clinic (Rochester MN) using short tandem repeat profiling upon receipt.

Techniques: Luciferase

Journal: Pharmaceuticals

Article Title: Experimental Evaluation of Anticancer Efficiency and Acute Toxicity of Anthrafuran for Oral Administration †

doi: 10.3390/ph13050081

Figure Lengend Snippet: Tumor growth inhibition of human s.c. xenografts of breast cancer T47D after oral administration of anthrafuran (70 mg/kg for 5 days).

Article Snippet: For the final study, a human breast cancer s.c. xenograft of T47D with an autocrine regulation of cell proliferation by estrogen receptor-alpha (ATCC ® HTB-133) was used.

Techniques: Inhibition

Journal: Genes

Article Title: Inhibitory Effect of Interleukin-24 on Programmed Death Ligand 1 Expression via a Eukaryotic Translation Initiation Factor 2 Alpha Kinase 2-Dependent Pathway in Human Triple-Negative Breast Cancer

doi: 10.3390/genes17030339

Figure Lengend Snippet: Comparison of PD-L1 protein levels across breast cancer cell lines. Western blot and quantification analyses of PD-L1 levels were performed on T47D, MCF7, MDA-MB-231, and MDA-MB-453 cell lines. ( A ) Cells were collected, proteins purified, and Western blot analysis was conducted to measure PD-L1 levels in these cell lines. β-actin was used as a loading control. The figure shown is representative of four independent experiments. ( B ) Representative Western blot showing PD-L1 protein levels under the indicated experimental conditions. Band intensities were quantified using the Odyssey Imaging System software v.5.2 (LI-COR Biosciences, Lincoln, NE, USA), and PD-L1 expression in each sample was normalized to the corresponding β-actin signal. The bar graph shows the mean ± SEM from four independent experiments. Group comparisons were conducted with one-way ANOVA and subsequent Tukey’s post hoc test. * p < 0.05, ns: not significant.

Article Snippet: All the breast cancer cell lines in this study, T47D (ATCC HTB-133), MCF7 (ATCC HTB-22), MDA-MB-453 (ATCC HTB-131), and MDA-MB-231 (ATCC HTB-26) are standard human breast cancer cell lines commonly used in cancer research, each representing distinct breast cancer subtypes and exhibiting varying characteristics, including hormone receptor status, epithelial or mesenchymal phenotype, and invasiveness.

Techniques: Comparison, Western Blot, Purification, Control, Imaging, Software, Expressing