Journal: Scientific Reports

Article Title: Interbase-FRET binding assay for pre-microRNAs

doi: 10.1038/s41598-021-88922-0

Figure Lengend Snippet: Sequences of oligoribonucleotides used in this study.

Article Snippet: Biotinylated oligoribonucleotide was immobilized on a streptavidin-coated biosensor chip (XanTec Bioanalytics GmbH).

Techniques: Sequencing

Journal: Scientific Reports

Article Title: Interbase-FRET binding assay for pre-microRNAs

doi: 10.1038/s41598-021-88922-0

Figure Lengend Snippet: Neomycin binding to pre-miR-21. ( b ) ITC data showing heat evolution upon titrating neomycin to oligoribonucleotide 2 (10 µM). ( b ) Integrated peak areas from panel ( a ) versus neomycin: 2 molar ratio for the successive additions and the fitted curve (solid line, assuming a 1:1 binding) yielding a K d of 5.2 ± 0.8 µM. ( c ) Neomycin binding to oligoribonucleotide 3 at 10 different concentrations using SPR, where the green marked region shows steady-state conditions from which K d values are determined. ( d ) The fitted curve (solid line, assuming an equivalent affinity for all binding sites) from panel ( c ) yielding an average K d of 3.9 ± 2.8 µM.

Article Snippet: Biotinylated oligoribonucleotide was immobilized on a streptavidin-coated biosensor chip (XanTec Bioanalytics GmbH).

Techniques: Binding Assay