Journal: Neural Regeneration Research

Article Title: The circ_0002538/miR-138-5p/plasmolipin axis regulates Schwann cell migration and myelination in diabetic peripheral neuropathy

doi: 10.4103/1673-5374.355979

Figure Lengend Snippet: Characterization of circ_0002538 and its function in SCs. (A) Hierarchical clustering analyses of DEcircRNAs ( n = 3). (B, C) RT-PCR verified five circRNAs with upregulated expression and six circRNAs with downregulated expression, and the results were consistent with the RNA-seq data ( n = 12). The red dotted box highlights the circRNA of interest. Y -axis: Fold changes in circRNA expression compared with the non-diabetic group. * P < 0.05, ** P < 0.01, vs . non-diabetic group (independent-sample t-test). (D) Schematic diagram showing that circ_0002538 was formed by the circularization of KLHL8 exon 2. The red arrow represents the “head-to-tail” splicing site of circ_0002538, confirmed by Sanger sequencing. (E) We used divergent primers and convergent primers to amplify circ_0002538 in cDNA and gDNA. We used β-actin as a negative control. (F) circ_0002538 and KLHL8 mRNA in SCs were detected via RT-PCR after incubation with or without RNase R. Y -axis: fold changes in RNA expression compared with the mock group. *** P < 0.001, vs . mock group (independent-sample t -test). (G) circ_0002538 and KLHL8 mRNA levels were evaluated in the sh-circ_0002538-transfected SCs via RT-PCR. Y -axis: fold changes in RNA expression compared with the sh-NC group. *** P < 0.001, vs . sh-NC group. (H, I) The migrating number of SCs in the sh-circ_0002538 group was lower than that in the sh-NC group in the Transwell assays. ** P < 0.01, vs . sh-NC group (independent-sample t -test). Scale bars: 100 μm. All bar graphs represent the average of at least three independent replicates, and the error bars are the SD. cDNA: Complementary DNA; DPN: diabetic peripheral neuropathy; gDNA: genomic DNA; KLHL8: Kelch-like family member 8; sh-circ_0002538: short hairpin RNA for circ_0002538; sh-NC: normal control for short hairpin RNA.

Article Snippet: To detect the combination of circRNAs and miRNAs, we performed RNA pulldown assays with biotinylated probes according to the manufacturer’s protocol (MCE, Shanghai, China, Cat# HY-K0208).

Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, RNA Sequencing, Sequencing, Negative Control, Incubation, RNA Expression, Transfection, shRNA, Control

Journal: Cell Genomics

Article Title: Chromatin topology and distal elements underlie divergent cell-type-specific regulation of 9p21 locus cell cycle genes

doi: 10.1016/j.xgen.2026.101304

Figure Lengend Snippet:

Article Snippet: The library pool was hybridized to the biotinylated probe panel overnight, after which streptavidin beads (Twist Bioscience, 100983) were used to pull down probes with hybridized ligated fragments and then washed (Twist Bioscience, 104178) to remove unbound fragments.

Techniques: Virus, Recombinant, SYBR Green Assay, Reverse Transcription, Protease Inhibitor, Multiplex Assay, Hybridization, Binding Assay, Library Amplification, DNA Purification, Plasmid Preparation, Gel Purification, Sensitive Assay, Expressing, Generated, Software, Transfection