Journal: International journal of molecular sciences

Article Title: 3D Tumor Models in Urology.

doi: 10.3390/ijms24076232

Figure Lengend Snippet: Figure 1. Proliferation of HT-1376 in strata membranes. Cells were grown in Alvetex Strata, fixed with PFA after 3 days (a), 13 days (b), and 19 days (c,d), embedded in paraffin, cut into 7 µm thick sections and labeled with fluorescent dyes; cell nuclei blue, beta-actin green, Ki-67 red; (a) HT-1376 cells already migrate from the apical side (asterisk) into the membrane (arrows), the proliferation is moderate (red nuclei); (b,c) many more cells are found within the membrane and the proliferation rate is especially high in those migrating cells; (d) higher magnification shows the formation of a superficial multilayered tissue-like structure and indicates the migration of single cells through the membranous pores (arrows); images taken with an CKX53 microscope (Olympus, Hamburg, Germany); calculation of the proliferation rate using Image J. [23]; (d) scale bar indicates 20 µm.

Article Snippet: We cultured the bladder carcinoma cell line HT-1376 (DSMZ, Braunschweig, Germany) on Alvetex Strata (AMSBIO, AMS Biotechnology Ltd., Abingdon, UK), a fine-pored membrane for three-dimensional cell cultivation.

Techniques: Labeling, Membrane, Migration, Microscopy

Journal: Facies

Article Title: Devonian to Carboniferous continental-scale carbonate turnover in Western Laurentia (North America): upwelling or climate cooling?

doi: 10.1007/s10347-022-00653-4

Figure Lengend Snippet: Upper Devonian and Lower Mississippian microfacies of Western Laurentia and depositional environments

Article Snippet: Remarkable exposures of hundreds of metres thick Upper Devonian and Lower Carboniferous strata in cliff-forming outcrops extending from western Canada to the Great Basin of southern Nevada provide a bountiful record of depositional changes to investigate the main driver for carbonate turnover.

Techniques: Western Blot, Sedimentation, Algae

Journal: Marine Drugs

Article Title: HPLC-HRMS Quantification of the Ichthyotoxin Karmitoxin from Karlodinium armiger

doi: 10.3390/md15090278

Figure Lengend Snippet: Combined integrated peak areas of monitored adducts for each of the external standards: chloramphenicol ([M + H] + , [M + Na] + , [3M + Fe(II)] 2+ , [2M − 2H + Fe(III)] + ) and valinomycin ([M + H] + , [M + NH 4 ] + , [M + Na] + ), monitored during the HPLC-QTOF-MS analysis of samples prepared by lyophilisation, and by solid phase extraction Strata-X.

Article Snippet: Figure S1: Calibration curve of karmitoxin in methanol; Figure S2: Relationship between karmitoxin and phototrophic cell density; Figure S3: Relationship between karmitoxin and mixotrophic cell density; Figure S4: Calibration curve of fumonisin B 1 ; Figure S5: Calibration curve of fumonisin B 2 ; Figure S6: Strata-X prepared sample with Bruker QTOF analysis; Table S1: Details of the amphidinol-like metabolites from dinoflagellates; Table S2: Concentration of karmitoxin per cell.

Techniques: