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Image Search Results
Journal: iScience
Article Title: Mitochondrial complex I inhibitors suppress tumor growth through concomitant acidification of the intra- and extracellular environment
doi: 10.1016/j.isci.2021.103497
Figure Lengend Snippet: Key resources table
Article Snippet:
Techniques: Recombinant, Synthesized, Staining, Isolation, Activity Assay, Lactate Assay, Software
Journal: Stem Cell Research & Therapy
Article Title: Translational models of 3-D organoids and cancer stem cells in gastric cancer research
doi: 10.1186/s13287-021-02521-4
Figure Lengend Snippet: Effect of ROCK inhibitor in gastric organoid culture. The rock inhibitor supplied in culture medium maintained the growth and morphologies of the organoids well and helped to avoid the culture-damages caused by the culture-environments. ( a ) The cultivation in the absence of the ROCK inhibitor (left lane) caused the growth of the organoids slowly. ( b ) Addition of ROCK inhibitor (10 µM; right panel) resulted in the growth of the organoids 1.5–2.0-fold faster compared with the control organoids (left panel). Human adenocarcinoma of stomach organoid lines, HCM-BROD-0208-C16 cancer model primary adenocarcinoma of stomach was purchased from American Type Culture Collection (PDM-146™; ATCC, VA, USA) and cultured as its recommended protocol (bright view, scale bar = 100 μm). ( c ) The stomach organoids were generated by seeding in 96-well plates and grown 2 days ROCK inhibitor (n = 6): Th organoids were incubated with CellTiter-Glo®3D reagent (G9681, Promega, Madison, WL, USA) prior to culture medium addition, and results were acquired to assay (** P < 0.01).
Article Snippet:
Techniques: Control, Cell Culture, Generated, Incubation
Journal: Stem Cell Research & Therapy
Article Title: Translational models of 3-D organoids and cancer stem cells in gastric cancer research
doi: 10.1186/s13287-021-02521-4
Figure Lengend Snippet: Effect of niche factors in gastric organoid culture. The concentration of R-SPONDIN 1 in the gastric cancer organoids culture was 10%, and the concentration of Wnt3a in the cancer organoids was 20% (panel a ) and 10% (panel b ). Human adenocarcinoma of stomach organoid lines, HCM-BROD-0208-C16 cancer model primary adenocarcinoma of stomach was purchased from American Type Culture Collection (PDM-146™; ATCC, USA) and cultured as its recommended protocol (bright view images scale bar = 50 μm). ( c ) The stomach organoids were generated by seeding in 96-well plates and grown 2 days at different percentages of Wnt (n = 6). Th organoids were incubated with CellTiter-Glo®3D reagent (G9681, Promega, Madison, WL,USA) prior to culture medium addition, and results were acquired to assay (*** P < 0.001),
Article Snippet:
Techniques: Concentration Assay, Cell Culture, Generated, Incubation