stim 1 Search Results


95
Cell Signaling Technology Inc rabbit anti stim1
Rabbit Anti Stim1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti stim1/product/Cell Signaling Technology Inc
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rabbit anti stim1 - by Bioz Stars, 2026-04
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90
Addgene inc human stim1 cfp plasmid
Figure 9. EHD2 regulates SOCE through <t>STIM1-Orai1.</t> (A) CFP-STIM1-trasnfected cells were analyzed for plasma membrane proximal fluorescent puncta by TIRF microscopy, without (control) or with thapsigargin treatment (2.5 μM, 5 min). Left, representative TIRF images; Right, Mean +/- SEM of STIM1 puncta/cell, ** p<0.01. Scale bar, 5 μm. (B) Immunoblotting to show comparable total STIM1 and Orai1 levels in control vs EHD2-KO TNBC lines; Hsc70, loading control. (C) Reduced cell surface levels of Orai1 in EHD2-KO cells. Live cell surface biotinylated cell Orai-1 immunoprecipitates blotted
Human Stim1 Cfp Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human stim1 cfp plasmid/product/Addgene inc
Average 90 stars, based on 1 article reviews
human stim1 cfp plasmid - by Bioz Stars, 2026-04
90/100 stars
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93
Addgene inc dr tobius mayer
Figure 9. EHD2 regulates SOCE through <t>STIM1-Orai1.</t> (A) CFP-STIM1-trasnfected cells were analyzed for plasma membrane proximal fluorescent puncta by TIRF microscopy, without (control) or with thapsigargin treatment (2.5 μM, 5 min). Left, representative TIRF images; Right, Mean +/- SEM of STIM1 puncta/cell, ** p<0.01. Scale bar, 5 μm. (B) Immunoblotting to show comparable total STIM1 and Orai1 levels in control vs EHD2-KO TNBC lines; Hsc70, loading control. (C) Reduced cell surface levels of Orai1 in EHD2-KO cells. Live cell surface biotinylated cell Orai-1 immunoprecipitates blotted
Dr Tobius Mayer, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dr tobius mayer/product/Addgene inc
Average 93 stars, based on 1 article reviews
dr tobius mayer - by Bioz Stars, 2026-04
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90
ProSci Incorporated anti stim1 rabbit polyclonal antibody
Figure 6. Immunohistochemical identification of <t>STIM1</t> distribution. A, Staining for STIM1 (left panels) was uniformly distributed within cytoplasmic areas of cells identified as neuronal profiles through costaining with NeuN (middle panels). Only minimal nonspecific staining was present in fiber tracts. The scale bar applies to each panel. B, Morphometric analysis showed comparableintensity(arbitraryunits,recordedusingstandardizedimageacquisition)ofstain- inginneuronsofallsizes(ANOVA,p0.40).ShownaremeanSEM.C,STIM1(leftpanel)was also found in cellular components identified as satellite glial cells by their expression of glu- tamine synthase (GS) (middle panel). Identically placed arrows in the left and middle panels indicate cellular areas clearly identifiable as satellite glial cell cytoplasm that expresses STIM1. The scale bar applies to each panel.
Anti Stim1 Rabbit Polyclonal Antibody, supplied by ProSci Incorporated, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti stim1 rabbit polyclonal antibody/product/ProSci Incorporated
Average 90 stars, based on 1 article reviews
anti stim1 rabbit polyclonal antibody - by Bioz Stars, 2026-04
90/100 stars
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94
Proteintech stim1
Figure 6. Immunohistochemical identification of <t>STIM1</t> distribution. A, Staining for STIM1 (left panels) was uniformly distributed within cytoplasmic areas of cells identified as neuronal profiles through costaining with NeuN (middle panels). Only minimal nonspecific staining was present in fiber tracts. The scale bar applies to each panel. B, Morphometric analysis showed comparableintensity(arbitraryunits,recordedusingstandardizedimageacquisition)ofstain- inginneuronsofallsizes(ANOVA,p0.40).ShownaremeanSEM.C,STIM1(leftpanel)was also found in cellular components identified as satellite glial cells by their expression of glu- tamine synthase (GS) (middle panel). Identically placed arrows in the left and middle panels indicate cellular areas clearly identifiable as satellite glial cell cytoplasm that expresses STIM1. The scale bar applies to each panel.
Stim1, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/stim1/product/Proteintech
Average 94 stars, based on 1 article reviews
stim1 - by Bioz Stars, 2026-04
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91
Novus Biologicals mouse anti stim1
Figure 6. Immunohistochemical identification of <t>STIM1</t> distribution. A, Staining for STIM1 (left panels) was uniformly distributed within cytoplasmic areas of cells identified as neuronal profiles through costaining with NeuN (middle panels). Only minimal nonspecific staining was present in fiber tracts. The scale bar applies to each panel. B, Morphometric analysis showed comparableintensity(arbitraryunits,recordedusingstandardizedimageacquisition)ofstain- inginneuronsofallsizes(ANOVA,p0.40).ShownaremeanSEM.C,STIM1(leftpanel)was also found in cellular components identified as satellite glial cells by their expression of glu- tamine synthase (GS) (middle panel). Identically placed arrows in the left and middle panels indicate cellular areas clearly identifiable as satellite glial cell cytoplasm that expresses STIM1. The scale bar applies to each panel.
Mouse Anti Stim1, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 91 stars, based on 1 article reviews
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94
Proteintech bicinchoninic acid bca
Figure 6. Immunohistochemical identification of <t>STIM1</t> distribution. A, Staining for STIM1 (left panels) was uniformly distributed within cytoplasmic areas of cells identified as neuronal profiles through costaining with NeuN (middle panels). Only minimal nonspecific staining was present in fiber tracts. The scale bar applies to each panel. B, Morphometric analysis showed comparableintensity(arbitraryunits,recordedusingstandardizedimageacquisition)ofstain- inginneuronsofallsizes(ANOVA,p0.40).ShownaremeanSEM.C,STIM1(leftpanel)was also found in cellular components identified as satellite glial cells by their expression of glu- tamine synthase (GS) (middle panel). Identically placed arrows in the left and middle panels indicate cellular areas clearly identifiable as satellite glial cell cytoplasm that expresses STIM1. The scale bar applies to each panel.
Bicinchoninic Acid Bca, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bicinchoninic acid bca/product/Proteintech
Average 94 stars, based on 1 article reviews
bicinchoninic acid bca - by Bioz Stars, 2026-04
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93
Santa Cruz Biotechnology antibodies against stim1
Fig. 1. <t>STIM1</t> overexpression results in increased Ca entry after store depletion. (A) Immunoblots for STIM1 and α-sarcomeric actin protein
Antibodies Against Stim1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/antibodies against stim1/product/Santa Cruz Biotechnology
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91
Addgene inc pcdna3 1 stim1 venus 173 c
Fig. 1. <t>STIM1</t> overexpression results in increased Ca entry after store depletion. (A) Immunoblots for STIM1 and α-sarcomeric actin protein
Pcdna3 1 Stim1 Venus 173 C, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pcdna3 1 stim1 venus 173 c/product/Addgene inc
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93
Addgene inc stim1 yfp
Fig. 1. <t>STIM1</t> overexpression results in increased Ca entry after store depletion. (A) Immunoblots for STIM1 and α-sarcomeric actin protein
Stim1 Yfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/stim1 yfp/product/Addgene inc
Average 93 stars, based on 1 article reviews
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93
Santa Cruz Biotechnology stim1 sirna
Fig. 1. <t>STIM1</t> overexpression results in increased Ca entry after store depletion. (A) Immunoblots for STIM1 and α-sarcomeric actin protein
Stim1 Sirna, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/stim1 sirna/product/Santa Cruz Biotechnology
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Image Search Results


Figure 9. EHD2 regulates SOCE through STIM1-Orai1. (A) CFP-STIM1-trasnfected cells were analyzed for plasma membrane proximal fluorescent puncta by TIRF microscopy, without (control) or with thapsigargin treatment (2.5 μM, 5 min). Left, representative TIRF images; Right, Mean +/- SEM of STIM1 puncta/cell, ** p<0.01. Scale bar, 5 μm. (B) Immunoblotting to show comparable total STIM1 and Orai1 levels in control vs EHD2-KO TNBC lines; Hsc70, loading control. (C) Reduced cell surface levels of Orai1 in EHD2-KO cells. Live cell surface biotinylated cell Orai-1 immunoprecipitates blotted

Journal: eLife

Article Title: EHD2 overexpression promotes tumorigenesis and metastasis in triple-negative breast cancer by regulating store-operated calcium entry

doi: 10.7554/elife.81288

Figure Lengend Snippet: Figure 9. EHD2 regulates SOCE through STIM1-Orai1. (A) CFP-STIM1-trasnfected cells were analyzed for plasma membrane proximal fluorescent puncta by TIRF microscopy, without (control) or with thapsigargin treatment (2.5 μM, 5 min). Left, representative TIRF images; Right, Mean +/- SEM of STIM1 puncta/cell, ** p<0.01. Scale bar, 5 μm. (B) Immunoblotting to show comparable total STIM1 and Orai1 levels in control vs EHD2-KO TNBC lines; Hsc70, loading control. (C) Reduced cell surface levels of Orai1 in EHD2-KO cells. Live cell surface biotinylated cell Orai-1 immunoprecipitates blotted

Article Snippet: Human STIM1- CFP plasmid (Prakriya et al., 2006) was from Addgene (#19755).

Techniques: Clinical Proteomics, Membrane, Microscopy, Control, Western Blot

Figure 6. Immunohistochemical identification of STIM1 distribution. A, Staining for STIM1 (left panels) was uniformly distributed within cytoplasmic areas of cells identified as neuronal profiles through costaining with NeuN (middle panels). Only minimal nonspecific staining was present in fiber tracts. The scale bar applies to each panel. B, Morphometric analysis showed comparableintensity(arbitraryunits,recordedusingstandardizedimageacquisition)ofstain- inginneuronsofallsizes(ANOVA,p0.40).ShownaremeanSEM.C,STIM1(leftpanel)was also found in cellular components identified as satellite glial cells by their expression of glu- tamine synthase (GS) (middle panel). Identically placed arrows in the left and middle panels indicate cellular areas clearly identifiable as satellite glial cell cytoplasm that expresses STIM1. The scale bar applies to each panel.

Journal: Journal of Neuroscience

Article Title: Store-Operated Ca2+ Entry in Sensory Neurons: Functional Role and the Effect of Painful Nerve Injury

doi: 10.1523/jneurosci.5053-10.2011

Figure Lengend Snippet: Figure 6. Immunohistochemical identification of STIM1 distribution. A, Staining for STIM1 (left panels) was uniformly distributed within cytoplasmic areas of cells identified as neuronal profiles through costaining with NeuN (middle panels). Only minimal nonspecific staining was present in fiber tracts. The scale bar applies to each panel. B, Morphometric analysis showed comparableintensity(arbitraryunits,recordedusingstandardizedimageacquisition)ofstain- inginneuronsofallsizes(ANOVA,p0.40).ShownaremeanSEM.C,STIM1(leftpanel)was also found in cellular components identified as satellite glial cells by their expression of glu- tamine synthase (GS) (middle panel). Identically placed arrows in the left and middle panels indicate cellular areas clearly identifiable as satellite glial cell cytoplasm that expresses STIM1. The scale bar applies to each panel.

Article Snippet: After blocking with 5% milk in TBST (Trisbuffered saline plus 0.1% Tween 20), blots were sequentially probed with anti- -Tubulin I mouse monoclonal antibody (1:20,000; Sigma-Aldrich; catalog #T7816), anti-STIM1 rabbit polyclonal antibody (1:500; ProSci; catalog #4119), and anti-Orai1 rabbit polyclonal antibody (1:1000; ProSci, catalog #4281).

Techniques: Immunohistochemical staining, Staining, Expressing

Figure 5. Identification of molecular components of store-operated Ca 2 channels. A, Western blotting detected Orai1 and STIM1 proteins in lysates from control (C) dorsal root ganglia (DRG), from fourth lumbar DRGs after fifth lumbar spinal nerve ligation (SNL L4), and fromaxotomizedfifthlumbarDRGsafterSNL(SNLL5).AdominantSTIM1bandattheexpected molecularweightisaccompaniedbyacloselycoherentsecondarybandthatothershaveshown to be sensitive to STIM1 knockdown by RNAi (Ong et al., 2007). (Typical of n 3.) B, Quantifi- cation of Western blots as fold difference compared with control showed no effect of injury on Orai1andSTIM1proteinlevels.ShownaremeanSEM.C,Quantificationoftranscriptlevelsby quantitativertPCRalsoshowednoeffectsofinjuryonOrai1andSTIM1expression.ForBandC, the numbers in bars indicate n.

Journal: Journal of Neuroscience

Article Title: Store-Operated Ca2+ Entry in Sensory Neurons: Functional Role and the Effect of Painful Nerve Injury

doi: 10.1523/jneurosci.5053-10.2011

Figure Lengend Snippet: Figure 5. Identification of molecular components of store-operated Ca 2 channels. A, Western blotting detected Orai1 and STIM1 proteins in lysates from control (C) dorsal root ganglia (DRG), from fourth lumbar DRGs after fifth lumbar spinal nerve ligation (SNL L4), and fromaxotomizedfifthlumbarDRGsafterSNL(SNLL5).AdominantSTIM1bandattheexpected molecularweightisaccompaniedbyacloselycoherentsecondarybandthatothershaveshown to be sensitive to STIM1 knockdown by RNAi (Ong et al., 2007). (Typical of n 3.) B, Quantifi- cation of Western blots as fold difference compared with control showed no effect of injury on Orai1andSTIM1proteinlevels.ShownaremeanSEM.C,Quantificationoftranscriptlevelsby quantitativertPCRalsoshowednoeffectsofinjuryonOrai1andSTIM1expression.ForBandC, the numbers in bars indicate n.

Article Snippet: After blocking with 5% milk in TBST (Trisbuffered saline plus 0.1% Tween 20), blots were sequentially probed with anti- -Tubulin I mouse monoclonal antibody (1:20,000; Sigma-Aldrich; catalog #T7816), anti-STIM1 rabbit polyclonal antibody (1:500; ProSci; catalog #4119), and anti-Orai1 rabbit polyclonal antibody (1:1000; ProSci, catalog #4281).

Techniques: Western Blot, Control, Ligation, Knockdown

Fig. 1. STIM1 overexpression results in increased Ca entry after store depletion. (A) Immunoblots for STIM1 and α-sarcomeric actin protein

Journal: Journal of molecular and cellular cardiology

Article Title: STIM1 elevation in the heart results in aberrant Ca²⁺ handling and cardiomyopathy.

doi: 10.1016/j.yjmcc.2015.07.032

Figure Lengend Snippet: Fig. 1. STIM1 overexpression results in increased Ca entry after store depletion. (A) Immunoblots for STIM1 and α-sarcomeric actin protein

Article Snippet: Antibodies against STIM1 (Millipore), Orai1 (Millipore), TRPC1 (Santa Cruz), TRPC3 (Alomone), TRPC6 (Alomone), CaMKIIδ (Abcam), phospho-RyR2 S2808 (Badrilla), phospho-RyR2 S2814 (Badrilla), total RyR (Santa Cruz), α-tubulin (Cell Signaling and Santa Cruz), NCX1 (Swant), SERCA2 (Badrilla), α-sarcomeric actin (Sigma), GAPDH (Fitzgerald Industries Intl.) and mitochondrial inner membrane proteins (MitoProfile total OXPHOS rodent western blot antibody cocktail, Abcam) were used.

Techniques: Over Expression, Western Blot

Fig. 2. STIM1 overexpression results in cardiac disease. (A) Fractional shortening (FS) measured by echocardiography, (B) hypertrophy

Journal: Journal of molecular and cellular cardiology

Article Title: STIM1 elevation in the heart results in aberrant Ca²⁺ handling and cardiomyopathy.

doi: 10.1016/j.yjmcc.2015.07.032

Figure Lengend Snippet: Fig. 2. STIM1 overexpression results in cardiac disease. (A) Fractional shortening (FS) measured by echocardiography, (B) hypertrophy

Article Snippet: Antibodies against STIM1 (Millipore), Orai1 (Millipore), TRPC1 (Santa Cruz), TRPC3 (Alomone), TRPC6 (Alomone), CaMKIIδ (Abcam), phospho-RyR2 S2808 (Badrilla), phospho-RyR2 S2814 (Badrilla), total RyR (Santa Cruz), α-tubulin (Cell Signaling and Santa Cruz), NCX1 (Swant), SERCA2 (Badrilla), α-sarcomeric actin (Sigma), GAPDH (Fitzgerald Industries Intl.) and mitochondrial inner membrane proteins (MitoProfile total OXPHOS rodent western blot antibody cocktail, Abcam) were used.

Techniques: Over Expression

Fig. 3. STIM1 transgenic mice are predisposed to increased cardiac disease after insult. (A) Fractional shortening (FS) measured by

Journal: Journal of molecular and cellular cardiology

Article Title: STIM1 elevation in the heart results in aberrant Ca²⁺ handling and cardiomyopathy.

doi: 10.1016/j.yjmcc.2015.07.032

Figure Lengend Snippet: Fig. 3. STIM1 transgenic mice are predisposed to increased cardiac disease after insult. (A) Fractional shortening (FS) measured by

Article Snippet: Antibodies against STIM1 (Millipore), Orai1 (Millipore), TRPC1 (Santa Cruz), TRPC3 (Alomone), TRPC6 (Alomone), CaMKIIδ (Abcam), phospho-RyR2 S2808 (Badrilla), phospho-RyR2 S2814 (Badrilla), total RyR (Santa Cruz), α-tubulin (Cell Signaling and Santa Cruz), NCX1 (Swant), SERCA2 (Badrilla), α-sarcomeric actin (Sigma), GAPDH (Fitzgerald Industries Intl.) and mitochondrial inner membrane proteins (MitoProfile total OXPHOS rodent western blot antibody cocktail, Abcam) were used.

Techniques: Transgenic Assay

Fig. 4. Cardiac myocytes isolated from STIM1 TG hearts show spontaneous Ca transients. (A) Ca traces from unstimulated Fura-2 loaded

Journal: Journal of molecular and cellular cardiology

Article Title: STIM1 elevation in the heart results in aberrant Ca²⁺ handling and cardiomyopathy.

doi: 10.1016/j.yjmcc.2015.07.032

Figure Lengend Snippet: Fig. 4. Cardiac myocytes isolated from STIM1 TG hearts show spontaneous Ca transients. (A) Ca traces from unstimulated Fura-2 loaded

Article Snippet: Antibodies against STIM1 (Millipore), Orai1 (Millipore), TRPC1 (Santa Cruz), TRPC3 (Alomone), TRPC6 (Alomone), CaMKIIδ (Abcam), phospho-RyR2 S2808 (Badrilla), phospho-RyR2 S2814 (Badrilla), total RyR (Santa Cruz), α-tubulin (Cell Signaling and Santa Cruz), NCX1 (Swant), SERCA2 (Badrilla), α-sarcomeric actin (Sigma), GAPDH (Fitzgerald Industries Intl.) and mitochondrial inner membrane proteins (MitoProfile total OXPHOS rodent western blot antibody cocktail, Abcam) were used.

Techniques: Isolation

Fig. 5. Increased Ca spark frequency in cardiac myocytes isolated from STIM1 TG hearts. (A) Representative confocal line scan images, as well

Journal: Journal of molecular and cellular cardiology

Article Title: STIM1 elevation in the heart results in aberrant Ca²⁺ handling and cardiomyopathy.

doi: 10.1016/j.yjmcc.2015.07.032

Figure Lengend Snippet: Fig. 5. Increased Ca spark frequency in cardiac myocytes isolated from STIM1 TG hearts. (A) Representative confocal line scan images, as well

Article Snippet: Antibodies against STIM1 (Millipore), Orai1 (Millipore), TRPC1 (Santa Cruz), TRPC3 (Alomone), TRPC6 (Alomone), CaMKIIδ (Abcam), phospho-RyR2 S2808 (Badrilla), phospho-RyR2 S2814 (Badrilla), total RyR (Santa Cruz), α-tubulin (Cell Signaling and Santa Cruz), NCX1 (Swant), SERCA2 (Badrilla), α-sarcomeric actin (Sigma), GAPDH (Fitzgerald Industries Intl.) and mitochondrial inner membrane proteins (MitoProfile total OXPHOS rodent western blot antibody cocktail, Abcam) were used.

Techniques: Isolation

Fig. 7. STIM1 TG mice show remodeling of Ca handling and signaling pathways. (A) Current–voltage relationships measured in whole-cell

Journal: Journal of molecular and cellular cardiology

Article Title: STIM1 elevation in the heart results in aberrant Ca²⁺ handling and cardiomyopathy.

doi: 10.1016/j.yjmcc.2015.07.032

Figure Lengend Snippet: Fig. 7. STIM1 TG mice show remodeling of Ca handling and signaling pathways. (A) Current–voltage relationships measured in whole-cell

Article Snippet: Antibodies against STIM1 (Millipore), Orai1 (Millipore), TRPC1 (Santa Cruz), TRPC3 (Alomone), TRPC6 (Alomone), CaMKIIδ (Abcam), phospho-RyR2 S2808 (Badrilla), phospho-RyR2 S2814 (Badrilla), total RyR (Santa Cruz), α-tubulin (Cell Signaling and Santa Cruz), NCX1 (Swant), SERCA2 (Badrilla), α-sarcomeric actin (Sigma), GAPDH (Fitzgerald Industries Intl.) and mitochondrial inner membrane proteins (MitoProfile total OXPHOS rodent western blot antibody cocktail, Abcam) were used.

Techniques: Protein-Protein interactions