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Cell Signaling Technology Inc
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Addgene inc
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Addgene inc
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ProSci Incorporated
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Proteintech
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Novus Biologicals
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Proteintech
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Santa Cruz Biotechnology
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Addgene inc
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Addgene inc
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Santa Cruz Biotechnology
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Image Search Results
Journal: eLife
Article Title: EHD2 overexpression promotes tumorigenesis and metastasis in triple-negative breast cancer by regulating store-operated calcium entry
doi: 10.7554/elife.81288
Figure Lengend Snippet: Figure 9. EHD2 regulates SOCE through STIM1-Orai1. (A) CFP-STIM1-trasnfected cells were analyzed for plasma membrane proximal fluorescent puncta by TIRF microscopy, without (control) or with thapsigargin treatment (2.5 μM, 5 min). Left, representative TIRF images; Right, Mean +/- SEM of STIM1 puncta/cell, ** p<0.01. Scale bar, 5 μm. (B) Immunoblotting to show comparable total STIM1 and Orai1 levels in control vs EHD2-KO TNBC lines; Hsc70, loading control. (C) Reduced cell surface levels of Orai1 in EHD2-KO cells. Live cell surface biotinylated cell Orai-1 immunoprecipitates blotted
Article Snippet:
Techniques: Clinical Proteomics, Membrane, Microscopy, Control, Western Blot
Journal: Journal of Neuroscience
Article Title: Store-Operated Ca2+ Entry in Sensory Neurons: Functional Role and the Effect of Painful Nerve Injury
doi: 10.1523/jneurosci.5053-10.2011
Figure Lengend Snippet: Figure 6. Immunohistochemical identification of STIM1 distribution. A, Staining for STIM1 (left panels) was uniformly distributed within cytoplasmic areas of cells identified as neuronal profiles through costaining with NeuN (middle panels). Only minimal nonspecific staining was present in fiber tracts. The scale bar applies to each panel. B, Morphometric analysis showed comparableintensity(arbitraryunits,recordedusingstandardizedimageacquisition)ofstain- inginneuronsofallsizes(ANOVA,p0.40).ShownaremeanSEM.C,STIM1(leftpanel)was also found in cellular components identified as satellite glial cells by their expression of glu- tamine synthase (GS) (middle panel). Identically placed arrows in the left and middle panels indicate cellular areas clearly identifiable as satellite glial cell cytoplasm that expresses STIM1. The scale bar applies to each panel.
Article Snippet: After blocking with 5% milk in TBST (Trisbuffered saline plus 0.1% Tween 20), blots were sequentially probed with anti- -Tubulin I mouse monoclonal antibody (1:20,000; Sigma-Aldrich; catalog #T7816),
Techniques: Immunohistochemical staining, Staining, Expressing
Journal: Journal of Neuroscience
Article Title: Store-Operated Ca2+ Entry in Sensory Neurons: Functional Role and the Effect of Painful Nerve Injury
doi: 10.1523/jneurosci.5053-10.2011
Figure Lengend Snippet: Figure 5. Identification of molecular components of store-operated Ca 2 channels. A, Western blotting detected Orai1 and STIM1 proteins in lysates from control (C) dorsal root ganglia (DRG), from fourth lumbar DRGs after fifth lumbar spinal nerve ligation (SNL L4), and fromaxotomizedfifthlumbarDRGsafterSNL(SNLL5).AdominantSTIM1bandattheexpected molecularweightisaccompaniedbyacloselycoherentsecondarybandthatothershaveshown to be sensitive to STIM1 knockdown by RNAi (Ong et al., 2007). (Typical of n 3.) B, Quantifi- cation of Western blots as fold difference compared with control showed no effect of injury on Orai1andSTIM1proteinlevels.ShownaremeanSEM.C,Quantificationoftranscriptlevelsby quantitativertPCRalsoshowednoeffectsofinjuryonOrai1andSTIM1expression.ForBandC, the numbers in bars indicate n.
Article Snippet: After blocking with 5% milk in TBST (Trisbuffered saline plus 0.1% Tween 20), blots were sequentially probed with anti- -Tubulin I mouse monoclonal antibody (1:20,000; Sigma-Aldrich; catalog #T7816),
Techniques: Western Blot, Control, Ligation, Knockdown
Journal: Journal of molecular and cellular cardiology
Article Title: STIM1 elevation in the heart results in aberrant Ca²⁺ handling and cardiomyopathy.
doi: 10.1016/j.yjmcc.2015.07.032
Figure Lengend Snippet: Fig. 1. STIM1 overexpression results in increased Ca entry after store depletion. (A) Immunoblots for STIM1 and α-sarcomeric actin protein
Article Snippet:
Techniques: Over Expression, Western Blot
Journal: Journal of molecular and cellular cardiology
Article Title: STIM1 elevation in the heart results in aberrant Ca²⁺ handling and cardiomyopathy.
doi: 10.1016/j.yjmcc.2015.07.032
Figure Lengend Snippet: Fig. 2. STIM1 overexpression results in cardiac disease. (A) Fractional shortening (FS) measured by echocardiography, (B) hypertrophy
Article Snippet:
Techniques: Over Expression
Journal: Journal of molecular and cellular cardiology
Article Title: STIM1 elevation in the heart results in aberrant Ca²⁺ handling and cardiomyopathy.
doi: 10.1016/j.yjmcc.2015.07.032
Figure Lengend Snippet: Fig. 3. STIM1 transgenic mice are predisposed to increased cardiac disease after insult. (A) Fractional shortening (FS) measured by
Article Snippet:
Techniques: Transgenic Assay
Journal: Journal of molecular and cellular cardiology
Article Title: STIM1 elevation in the heart results in aberrant Ca²⁺ handling and cardiomyopathy.
doi: 10.1016/j.yjmcc.2015.07.032
Figure Lengend Snippet: Fig. 4. Cardiac myocytes isolated from STIM1 TG hearts show spontaneous Ca transients. (A) Ca traces from unstimulated Fura-2 loaded
Article Snippet:
Techniques: Isolation
Journal: Journal of molecular and cellular cardiology
Article Title: STIM1 elevation in the heart results in aberrant Ca²⁺ handling and cardiomyopathy.
doi: 10.1016/j.yjmcc.2015.07.032
Figure Lengend Snippet: Fig. 5. Increased Ca spark frequency in cardiac myocytes isolated from STIM1 TG hearts. (A) Representative confocal line scan images, as well
Article Snippet:
Techniques: Isolation
Journal: Journal of molecular and cellular cardiology
Article Title: STIM1 elevation in the heart results in aberrant Ca²⁺ handling and cardiomyopathy.
doi: 10.1016/j.yjmcc.2015.07.032
Figure Lengend Snippet: Fig. 7. STIM1 TG mice show remodeling of Ca handling and signaling pathways. (A) Current–voltage relationships measured in whole-cell
Article Snippet:
Techniques: Protein-Protein interactions