sterile Search Results


catalog number fpe204500 11  (Guangzhou JET Bio-Filtration)
93
Guangzhou JET Bio-Filtration catalog number fpe204500 11
Catalog Number Fpe204500 11, supplied by Guangzhou JET Bio-Filtration, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sterile/Sterile+Vacuum+Filter/10__21769_slash_bioprotoc__5678-113-10-8
Average 93 stars, based on 1 article reviews
catalog number fpe204500 11 - by Bioz Stars, 2026-10
93/100 stars
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generuler 1 kb plus dna ladder  (Thermo Fisher)
98
Thermo Fisher generuler 1 kb plus dna ladder
Generuler 1 Kb Plus Dna Ladder, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sterile/Zeocin%5Et%2C+in+HEPES%2C+sterile-filtered/pmc12379159-163-32-46
Average 98 stars, based on 1 article reviews
generuler 1 kb plus dna ladder - by Bioz Stars, 2026-10
98/100 stars
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nitrocellulose membrane  (Danaher Inc)
98
Danaher Inc nitrocellulose membrane
FIG. 4. The p74–78-kDa protein is localized to the cytoplasm. A, serum-starved cells were untreated or treated -thrombin (0.4 units/ml; 15 min) and lysed, and then membrane, cytoplasm, and nuclear frac- tions were prepared as described under “Experimental Procedures.” 15 g of the sample were run on an 8% SDS-polyacrylamide gel, trans- ferred to <t>nitrocellulose,</t> and immunoblotted with the phospho-specific anti-Stat3 antibody. The positions of three distinct bands (74–78 kDa) are indicated by arrows. B and C are blots probed with anti-Stat3 antibody and anti-TGF- receptor antibody, respectively. These blots are representative of three independent experiments. Th, -thrombin; CR, cross-reactive.
Nitrocellulose Membrane, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sterile/Cellulose+Nitrate+Membrane%2C+plain%2C+sterile%2C+pore+size%2C+circle/10__1074_slash_jbc__m409043200-63-20-24
Average 98 stars, based on 1 article reviews
nitrocellulose membrane - by Bioz Stars, 2026-10
98/100 stars
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kinase enzyme  (Carna Inc)
94
Carna Inc kinase enzyme
FIG. 4. The p74–78-kDa protein is localized to the cytoplasm. A, serum-starved cells were untreated or treated -thrombin (0.4 units/ml; 15 min) and lysed, and then membrane, cytoplasm, and nuclear frac- tions were prepared as described under “Experimental Procedures.” 15 g of the sample were run on an 8% SDS-polyacrylamide gel, trans- ferred to <t>nitrocellulose,</t> and immunoblotted with the phospho-specific anti-Stat3 antibody. The positions of three distinct bands (74–78 kDa) are indicated by arrows. B and C are blots probed with anti-Stat3 antibody and anti-TGF- receptor antibody, respectively. These blots are representative of three independent experiments. Th, -thrombin; CR, cross-reactive.
Kinase Enzyme, supplied by Carna Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sterile/ZAK/us08119812-298-10-17
Average 94 stars, based on 1 article reviews
kinase enzyme - by Bioz Stars, 2026-10
94/100 stars
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sterile dmso  (Tocris)
96
Tocris sterile dmso
FIG. 4. The p74–78-kDa protein is localized to the cytoplasm. A, serum-starved cells were untreated or treated -thrombin (0.4 units/ml; 15 min) and lysed, and then membrane, cytoplasm, and nuclear frac- tions were prepared as described under “Experimental Procedures.” 15 g of the sample were run on an 8% SDS-polyacrylamide gel, trans- ferred to <t>nitrocellulose,</t> and immunoblotted with the phospho-specific anti-Stat3 antibody. The positions of three distinct bands (74–78 kDa) are indicated by arrows. B and C are blots probed with anti-Stat3 antibody and anti-TGF- receptor antibody, respectively. These blots are representative of three independent experiments. Th, -thrombin; CR, cross-reactive.
Sterile Dmso, supplied by Tocris, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sterile/DMSO%2C+sterile+filtered/pm34590912-51-11-13
Average 96 stars, based on 1 article reviews
sterile dmso - by Bioz Stars, 2026-10
96/100 stars
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sterile distilled water  (Thermo Fisher)
95
Thermo Fisher sterile distilled water
FIG. 4. The p74–78-kDa protein is localized to the cytoplasm. A, serum-starved cells were untreated or treated -thrombin (0.4 units/ml; 15 min) and lysed, and then membrane, cytoplasm, and nuclear frac- tions were prepared as described under “Experimental Procedures.” 15 g of the sample were run on an 8% SDS-polyacrylamide gel, trans- ferred to <t>nitrocellulose,</t> and immunoblotted with the phospho-specific anti-Stat3 antibody. The positions of three distinct bands (74–78 kDa) are indicated by arrows. B and C are blots probed with anti-Stat3 antibody and anti-TGF- receptor antibody, respectively. These blots are representative of three independent experiments. Th, -thrombin; CR, cross-reactive.
Sterile Distilled Water, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sterile/Kanamycin%2C+in+distilled+water%2C+sterile-filtered/pmc11181181-81-16-22
Average 95 stars, based on 1 article reviews
sterile distilled water - by Bioz Stars, 2026-10
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cells  (Thermo Fisher)
96
Thermo Fisher cells
FIG. 4. The p74–78-kDa protein is localized to the cytoplasm. A, serum-starved cells were untreated or treated -thrombin (0.4 units/ml; 15 min) and lysed, and then membrane, cytoplasm, and nuclear frac- tions were prepared as described under “Experimental Procedures.” 15 g of the sample were run on an 8% SDS-polyacrylamide gel, trans- ferred to <t>nitrocellulose,</t> and immunoblotted with the phospho-specific anti-Stat3 antibody. The positions of three distinct bands (74–78 kDa) are indicated by arrows. B and C are blots probed with anti-Stat3 antibody and anti-TGF- receptor antibody, respectively. These blots are representative of three independent experiments. Th, -thrombin; CR, cross-reactive.
Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sterile/Puromycin+dihydrochloride%2C+in+distilled+water%2C+sterile-filtered/pm42262924-783-0-8
Average 96 stars, based on 1 article reviews
cells - by Bioz Stars, 2026-10
96/100 stars
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blasticidin  (Thermo Fisher)
95
Thermo Fisher blasticidin
FIG. 4. The p74–78-kDa protein is localized to the cytoplasm. A, serum-starved cells were untreated or treated -thrombin (0.4 units/ml; 15 min) and lysed, and then membrane, cytoplasm, and nuclear frac- tions were prepared as described under “Experimental Procedures.” 15 g of the sample were run on an 8% SDS-polyacrylamide gel, trans- ferred to <t>nitrocellulose,</t> and immunoblotted with the phospho-specific anti-Stat3 antibody. The positions of three distinct bands (74–78 kDa) are indicated by arrows. B and C are blots probed with anti-Stat3 antibody and anti-TGF- receptor antibody, respectively. These blots are representative of three independent experiments. Th, -thrombin; CR, cross-reactive.
Blasticidin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sterile/Blasticidin+S+hydrochloride%2C+in+HEPES+buffer%2C+sterile-filtered/pmc11614702-53-9-11
Average 95 stars, based on 1 article reviews
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fibroblast outgrowth  (Thermo Fisher)
94
Thermo Fisher fibroblast outgrowth
(A) Western blot of <t>fibroblast</t> cell line lysates from control, and Family#1 father, female patient, and male patient. The affected patient cell lines did not show detectable CC2D1A expression. (B, C) Quantification of fibroblast starvation–induced ciliary length. The patients displayed fewer ciliated cells or shorter ciliary lengths. ns, not significant; P < 0.01 (**), P < 0.0001 (****). (D, E, F) Cultured and starved fibroblasts from Family#1 father, female patient, and male patient stained with DAPI (blue) to show nuclei, phalloidin, Alexa Fluor 488 (green) to show actin cytoskeleton, and anti-acetylated tubulin (red) to show ciliary axonemes. Cilia on the patient fibroblasts were less frequent and shorter than those on the control and father fibroblasts. Scale bar = 25 μm. Source data are available for this figure.
Fibroblast Outgrowth, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sterile/Amphotericin+B%2C+in+distilled+water%2C+sterile-filtered/pmc11339347-343-9-22
Average 94 stars, based on 1 article reviews
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1x phosphate buffered saline  (Thermo Fisher)
99
Thermo Fisher 1x phosphate buffered saline
(A) Western blot of <t>fibroblast</t> cell line lysates from control, and Family#1 father, female patient, and male patient. The affected patient cell lines did not show detectable CC2D1A expression. (B, C) Quantification of fibroblast starvation–induced ciliary length. The patients displayed fewer ciliated cells or shorter ciliary lengths. ns, not significant; P < 0.01 (**), P < 0.0001 (****). (D, E, F) Cultured and starved fibroblasts from Family#1 father, female patient, and male patient stained with DAPI (blue) to show nuclei, phalloidin, Alexa Fluor 488 (green) to show actin cytoskeleton, and anti-acetylated tubulin (red) to show ciliary axonemes. Cilia on the patient fibroblasts were less frequent and shorter than those on the control and father fibroblasts. Scale bar = 25 μm. Source data are available for this figure.
1x Phosphate Buffered Saline, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sterile/Phosphate-buffered+saline+(PBS%2C+1X)%2C+sterile-filtered/pm38111086-70-82-87
Average 99 stars, based on 1 article reviews
1x phosphate buffered saline - by Bioz Stars, 2026-10
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sterile spatula  (Eppendorf AG)
96
Eppendorf AG sterile spatula
(A) Western blot of <t>fibroblast</t> cell line lysates from control, and Family#1 father, female patient, and male patient. The affected patient cell lines did not show detectable CC2D1A expression. (B, C) Quantification of fibroblast starvation–induced ciliary length. The patients displayed fewer ciliated cells or shorter ciliary lengths. ns, not significant; P < 0.01 (**), P < 0.0001 (****). (D, E, F) Cultured and starved fibroblasts from Family#1 father, female patient, and male patient stained with DAPI (blue) to show nuclei, phalloidin, Alexa Fluor 488 (green) to show actin cytoskeleton, and anti-acetylated tubulin (red) to show ciliary axonemes. Cilia on the patient fibroblasts were less frequent and shorter than those on the control and father fibroblasts. Scale bar = 25 μm. Source data are available for this figure.
Sterile Spatula, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sterile/Tubes%2C+Sterile/pm30029618-89-8-24
Average 96 stars, based on 1 article reviews
sterile spatula - by Bioz Stars, 2026-10
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cell scrapers  (Genesee Scientific)
94
Genesee Scientific cell scrapers
(A) Western blot of <t>fibroblast</t> cell line lysates from control, and Family#1 father, female patient, and male patient. The affected patient cell lines did not show detectable CC2D1A expression. (B, C) Quantification of fibroblast starvation–induced ciliary length. The patients displayed fewer ciliated cells or shorter ciliary lengths. ns, not significant; P < 0.01 (**), P < 0.0001 (****). (D, E, F) Cultured and starved fibroblasts from Family#1 father, female patient, and male patient stained with DAPI (blue) to show nuclei, phalloidin, Alexa Fluor 488 (green) to show actin cytoskeleton, and anti-acetylated tubulin (red) to show ciliary axonemes. Cilia on the patient fibroblasts were less frequent and shorter than those on the control and father fibroblasts. Scale bar = 25 μm. Source data are available for this figure.
Cell Scrapers, supplied by Genesee Scientific, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sterile/Cell+Scrapers%2C+Sterile/bio_rxiv__64898__2026__01__15__699810-261-15-17
Average 94 stars, based on 1 article reviews
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Image Search Results


FIG. 4. The p74–78-kDa protein is localized to the cytoplasm. A, serum-starved cells were untreated or treated -thrombin (0.4 units/ml; 15 min) and lysed, and then membrane, cytoplasm, and nuclear frac- tions were prepared as described under “Experimental Procedures.” 15 g of the sample were run on an 8% SDS-polyacrylamide gel, trans- ferred to nitrocellulose, and immunoblotted with the phospho-specific anti-Stat3 antibody. The positions of three distinct bands (74–78 kDa) are indicated by arrows. B and C are blots probed with anti-Stat3 antibody and anti-TGF- receptor antibody, respectively. These blots are representative of three independent experiments. Th, -thrombin; CR, cross-reactive.

Journal: Journal of Biological Chemistry

Article Title: α-Thrombin Rapidly Induces Tyrosine Phosphorylation of a Novel, 74–78-kDa Stress Response Protein(s) in Lung Fibroblast Cells

doi: 10.1074/jbc.m409043200

Figure Lengend Snippet: FIG. 4. The p74–78-kDa protein is localized to the cytoplasm. A, serum-starved cells were untreated or treated -thrombin (0.4 units/ml; 15 min) and lysed, and then membrane, cytoplasm, and nuclear frac- tions were prepared as described under “Experimental Procedures.” 15 g of the sample were run on an 8% SDS-polyacrylamide gel, trans- ferred to nitrocellulose, and immunoblotted with the phospho-specific anti-Stat3 antibody. The positions of three distinct bands (74–78 kDa) are indicated by arrows. B and C are blots probed with anti-Stat3 antibody and anti-TGF- receptor antibody, respectively. These blots are representative of three independent experiments. Th, -thrombin; CR, cross-reactive.

Article Snippet: IL-6, PDGF, transforming growth factor- (TGF- ), and basic fibroblast growth factor (FGF) were obtained from R & D Systems; nitrocellulose membrane was from Amersham Biosciences; phospho-specific antiStat3 antibody was from BIOSOURCE; anti-Stat3 antibody and antiE2F-1 antibody were from Santa Cruz Biotechnology; anti-phosphotyrosine antibody was from Upstate Biotechnology, Inc.; anti-GRP antibody was from Oxford Biomedical Research; goat anti-rabbit IgG and rabbit anti-mouse IgG were from Bio-Rad; all other chemicals were either from Sigma or Fisher.

Techniques: Membrane

(A) Western blot of fibroblast cell line lysates from control, and Family#1 father, female patient, and male patient. The affected patient cell lines did not show detectable CC2D1A expression. (B, C) Quantification of fibroblast starvation–induced ciliary length. The patients displayed fewer ciliated cells or shorter ciliary lengths. ns, not significant; P < 0.01 (**), P < 0.0001 (****). (D, E, F) Cultured and starved fibroblasts from Family#1 father, female patient, and male patient stained with DAPI (blue) to show nuclei, phalloidin, Alexa Fluor 488 (green) to show actin cytoskeleton, and anti-acetylated tubulin (red) to show ciliary axonemes. Cilia on the patient fibroblasts were less frequent and shorter than those on the control and father fibroblasts. Scale bar = 25 μm. Source data are available for this figure.

Journal: Life Science Alliance

Article Title: CC2D1A causes ciliopathy, intellectual disability, heterotaxy, renal dysplasia, and abnormal CSF flow

doi: 10.26508/lsa.202402708

Figure Lengend Snippet: (A) Western blot of fibroblast cell line lysates from control, and Family#1 father, female patient, and male patient. The affected patient cell lines did not show detectable CC2D1A expression. (B, C) Quantification of fibroblast starvation–induced ciliary length. The patients displayed fewer ciliated cells or shorter ciliary lengths. ns, not significant; P < 0.01 (**), P < 0.0001 (****). (D, E, F) Cultured and starved fibroblasts from Family#1 father, female patient, and male patient stained with DAPI (blue) to show nuclei, phalloidin, Alexa Fluor 488 (green) to show actin cytoskeleton, and anti-acetylated tubulin (red) to show ciliary axonemes. Cilia on the patient fibroblasts were less frequent and shorter than those on the control and father fibroblasts. Scale bar = 25 μm. Source data are available for this figure.

Article Snippet: The fibroblasts were cultured for ∼4 wk until enough fibroblast outgrowth had occurred to allow for additional cell passage (amphotericin B [J67049.AD; Thermo Fisher Scientific], DMEM [#21068028; Gibco], L-glutamine [#25030081; Gibco], sodium pyruvate [#11360070; Gibco], penicillin–streptomycin [#10378016; Gibco], FBS [#10270-106; Gibco]).

Techniques: Western Blot, Control, Expressing, Cell Culture, Staining