Journal: Oncogene

Article Title: ZBTB7A prevents RUNX1-RUNX1T1-dependent clonal expansion of human hematopoietic stem and progenitor cells

doi: 10.1038/s41388-020-1209-4

Figure Lengend Snippet: Flow cytometry measurements of human bone marrow CD34+ cells stably expressing ZBTB7A WT or mutants are shown. a Representative results of cells primed for differentiation into erythrocytes using StemSpan Erythroid Expansion Supplement for 7 days. b Summary of three independent experiments. c Representative results of cells primed for differentiation into monocytes and granulocytes using HemaTox Myeloid Kit for 7 days. d Summary of three independent experiments. e Schematic representation of the effects of ZBTB7A and RUNX1–RUNX1T1 expression on hematopoietic linage commitment.

Article Snippet: A total of 5000 Human CD34+ bone marrow cells were seeded either in StemSpan SFEM with StemSpan Erythroid Expansion Supplement or HemaTox Myeloid Kit (StemCell Technologies) in a 96-well plate.

Techniques: Flow Cytometry, Stable Transfection, Expressing

Journal: Frontiers in Immunology

Article Title: Icaritin Induces Anti-tumor Immune Responses in Hepatocellular Carcinoma by Inhibiting Splenic Myeloid-Derived Suppressor Cell Generation

doi: 10.3389/fimmu.2021.609295

Figure Lengend Snippet: Icaritin reduces the accumulation of HSPCs in the spleen of Hepa mice. Spleen weight and total cell number from orthotopic (A) and subcutaneous (B) Hepa mice. Numbers of splenic LSK and LK cells from orthotopic (C) and subcutaneous (D) Hepa mice. Differences between groups were examined for statistical significance by Student's t -test. Data were pooled from two experiments and n = 8 mice per group. * P < 0.05, ** P < 0.01, *** P < 0.001. HSPCs, hematopoietic stem and progenitor cells; Hepa, Hepa1-6 cells; ICT, icaritin; Veh, Vehicle; LSK, Lin lo/− Sca-1 + c-Kit hi ; LK, Lin lo/− Sca-1 − c-Kit hi .

Article Snippet: In brief, human CD34 + cells were isolated from the cord blood mononuclear cells using a direct CD34 progenitor cell isolation kit (Miltenyi Biotec), and cultured in hematopoietic stem cell expansion media (StemSpan SFEM; Stem Cell Technologies) supplemented with 100 ng/mL stem cell factor, 100 ng/mL Fms-like tyrosine kinase 3, 100 ng/mL thrombopoietin, and 20 ng/mL IL-3 (R&D Systems) for 9 days ( ).

Techniques:

Journal: Frontiers in Immunology

Article Title: Icaritin Induces Anti-tumor Immune Responses in Hepatocellular Carcinoma by Inhibiting Splenic Myeloid-Derived Suppressor Cell Generation

doi: 10.3389/fimmu.2021.609295

Figure Lengend Snippet: Icaritin inhibits the generation of human PMN-MDSCs in vitro . Freshly isolated human CD34 + cells from cord blood mononuclear cells were cultured in hematopoietic stem cell expansion media for 8–10 days. The expanded cells were cultured with combined IL-6 and G-CSF in complete medium with 0.1% DMSO (vehicle) or icaritin (2.5 μM) for 3 days. (A) Flow cytometric analysis of CD115 and HLA-DR expression on CD14 + or CD15 + cells. (B) Frequencies of PMN-MDSCs (CD15 + CD115 + ) and M-MDSCs (CD14 + HLA-DR − ). Differences between groups were analyzed using one-way ANOVA, and corrected by Dunnett's test. Data are representative of three experiments. ** P < 0.01. Veh, Vehicle; ICT, Icaritin.

Article Snippet: In brief, human CD34 + cells were isolated from the cord blood mononuclear cells using a direct CD34 progenitor cell isolation kit (Miltenyi Biotec), and cultured in hematopoietic stem cell expansion media (StemSpan SFEM; Stem Cell Technologies) supplemented with 100 ng/mL stem cell factor, 100 ng/mL Fms-like tyrosine kinase 3, 100 ng/mL thrombopoietin, and 20 ng/mL IL-3 (R&D Systems) for 9 days ( ).

Techniques: In Vitro, Isolation, Cell Culture, Expressing