Journal: The Journal of clinical endocrinology and metabolism

Article Title: Interleukin-12 and its free p40 subunit regulate immune recognition of endometrial cells: potential role in endometriosis.

doi: 10.1210/jcem.83.3.4612

Figure Lengend Snippet: FIG. 7. p40 inhibits IL-12-induced phosphorylation of STAT4 pro- teins. NK3.3 cells were incubated overnight with medium alone or p40 (30 ng/mL), and then induced with IL-12 (10 ng/mL) for 15 min at 37 C. The cell lysates were immunoprecipitated with anti-STAT4 mAb, separated by SDS-PAGE, and transferred to a membrane. Phospho- tyrosine was detected by antiphosphotyrosine antibody. The filters were then reprobed with anti STAT4 mAb to determine the amount of STAT4 in each lane. The experiment shown is representative of three that gave similar results.

Article Snippet: The membranes were blocked by incubating overnight at 4 C with TBS-T (20 mmol/L Tris base, 137 mmol/L NaCl, and 0.1% Tween-20, pH 7.6) and 5% nonfat dry milk and then probed at room temperature with antiphosphotyrosine (Upstate Biotechnology, Lake Placid, NY), followed by antimouse horseradish peroxidase (Amersham Life Science, Arlington Heights, IL) or with a anti-STAT4 mAb (Santa Cruz Biotechnology) followed by antirabbit horseradish peroxidase.

Techniques: Phospho-proteomics, Incubation, Immunoprecipitation, SDS Page, Membrane

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Overexpression of NLRP12 enhances macrophage immune response and alleviates herpes simplex keratitis

doi: 10.3389/fcimb.2024.1416105

Figure Lengend Snippet: NLRP12 enhances antiviral functions through IL-18 downstream JAK-STAT signaling. (A) Protein levels of STAT1, p-STAT1, STAT4, pSTAT4 were assessed at 8h, 12h and 24h post-infection in both groups. Western blot bands were normalized to β-actin and compared against controls (n=3 per group). (B) Immunofluorescence images demonstrating MHC II + cell staining. All of the data are representative of at least three independent experiments. Data are presented as the mean ± SEM. Statistical differences were determined using two-way ANOVA (A) . ns, not significnat, *P <0.05, ***P <0.001, ****P <0.0001.

Article Snippet: The membranes were then blocked with Y-Tec 5min Rapid Blocking Buffer (YWB0501, Yoche, China) and incubated overnight with primary antibodies against NLRP12 (1:1000, YT7568, Immunoway, USA), HSV-gB (1:500, sc-56987, Santa Cruz, USA), HSV-gD (1:500, sc-21719, Santa Cruz, USA), CASP1 (1:2000, 81482–1-RR, Proteintech, China), N-GSDMD (1:2000, ab215203, Abcam, UK), clv-CASP1 (1:500, AY0406, Abways, China), IL-18 (1:500, A1115, ABclonal, China), IL-1β (1:1000, ab254360, Abcam, UK), STAT1 (1:1000, CY5227, Abways, China), STAT1 (Phospho-S727) (1:1000, BM4541, Boster, USA), STAT4 (1:1000, BA0622–2, Boster, USA), Phospho-STAT4 (Tyr693) (1:1000, CY6503, Abways, China), and β-actin (1:20000, 81115–1-RR, Proteintech, China).

Techniques: Infection, Western Blot, Immunofluorescence, Staining

Journal: Journal of Inflammation Research

Article Title: Genetic Polymorphisms in Genes Involved in the Type I Interferon System (IFIH1/MDA-5, TNFAIP3/A20, and STAT4): Association with SLE Risk in Egyptian Children and Adolescents

doi: 10.2147/JIR.S309008

Figure Lengend Snippet: Distribution of IFIH1, TNFAIP3 and STAT4 Genotype and Allele Frequencies in the Studied SLE (Systemic Lupus Erythematosus) Patients and Healthy Control Subjects

Article Snippet: Genotyping of the IFIH1 (ID: C__25984418_10 for rs3747517 C/T), TNFAIP3 (ID: C____884105_20 for rs610604 G/T) and STAT4 (ID: C__29882391_10 for rs7574865 G/T) variants were performed using the TaqMan ® genotyping assays (Thermo Fisher Scientific Inc., Waltham, MA, USA).

Techniques: Control

Journal: Journal of Inflammation Research

Article Title: Genetic Polymorphisms in Genes Involved in the Type I Interferon System (IFIH1/MDA-5, TNFAIP3/A20, and STAT4): Association with SLE Risk in Egyptian Children and Adolescents

doi: 10.2147/JIR.S309008

Figure Lengend Snippet: Association of IFIH1 rs3747517 C/T, TNFAIP3 rs610604 G/T and STAT4 rs7574865 G/T Genotypes with SLE Clinical or Laboratory Features

Article Snippet: Genotyping of the IFIH1 (ID: C__25984418_10 for rs3747517 C/T), TNFAIP3 (ID: C____884105_20 for rs610604 G/T) and STAT4 (ID: C__29882391_10 for rs7574865 G/T) variants were performed using the TaqMan ® genotyping assays (Thermo Fisher Scientific Inc., Waltham, MA, USA).

Techniques:

Journal: Journal of Inflammation Research

Article Title: Genetic Polymorphisms in Genes Involved in the Type I Interferon System (IFIH1/MDA-5, TNFAIP3/A20, and STAT4): Association with SLE Risk in Egyptian Children and Adolescents

doi: 10.2147/JIR.S309008

Figure Lengend Snippet: Regression Analysis for Prediction of SLE Susceptibility

Article Snippet: Genotyping of the IFIH1 (ID: C__25984418_10 for rs3747517 C/T), TNFAIP3 (ID: C____884105_20 for rs610604 G/T) and STAT4 (ID: C__29882391_10 for rs7574865 G/T) variants were performed using the TaqMan ® genotyping assays (Thermo Fisher Scientific Inc., Waltham, MA, USA).

Techniques: