stargazin Search Results


sem  (Alomone Labs)
90
Alomone Labs sem
Numbers of primary antibodies per synaptosome for over 100 targets.
Sem, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stargazin/Anti-Stargazin+Antibody/pmc06172260-83-5-11
Average 90 stars, based on 1 article reviews
sem - by Bioz Stars, 2026-10
90/100 stars
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stargazin gfp lovpep sequence  (Addgene inc)
93
Addgene inc stargazin gfp lovpep sequence
Figure 5. Junction elongation induces Rho flares. (A) Graph showing that calcium flash (GCaMP6m) follows junction elongation (verted-to-vertex length) and precedes Rho-mediated junction contraction and stabilization of length. Time 0 s represents start of Rho flare. Shaded region represents SEM; n = 16 flares, 13 embryos, 10 experiments. (B) Schematic of mosaic injection and regional activation of Rho-mediated contractility using optogenetics. Top: Schematic showing the mosaic injection of optogenetic constructs (prGEF and <t>LOVpep)</t> injected into one cell, while active Rho probe is injected in both cells at two-cell stage. Bottom: Regional activation of RhoA using optogenetics in region of stimulation (shaded gray box) and quantification of junction length and frequency of Rho flares in region of observation (dotted blue box). (C) Cell view of an embryo mosaically-expressing prGEF (2xPDZ-YFP-LARG(DH), yellow) and active Rho probe (mCherry-2xrGBD, gray). (C9) Cell view of Rho probe in the embryo from C. Regional stimulation (white dashed box) induces Rho flares in areas neighboring the region of stimulation (yellow arrowheads). (D) Quantification of Rho flares in the area outside the region of stimulation shown in C9. Frequency of Rho flares occurring pre-stimulation (0–300 s) and post-stimulation (600–900 s) are matched for color and shape. Significance calculated using unpaired two-tailed t test; n = 6 embryos, 4 experiments. (E) Time-lapse montage of a junction highlighted in C9 (yellow box) expressing active Rho probe (mCherry- 2xrGBD). Following optogenetic stimulation (indicated by gray shaded box), an increase in junction length precedes Rho flare activation (yellow arrowhead). Junction length measured from vertex to vertex is indicated under each panel.
Stargazin Gfp Lovpep Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stargazin/Stargazin-GFP-LOVpep+(Plasmid+%2380406)/pm35254388-261-11-14
Average 93 stars, based on 1 article reviews
stargazin gfp lovpep sequence - by Bioz Stars, 2026-10
93/100 stars
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drugs rabbit anti stargazin antibody  (Cell Signaling Technology Inc)
93
Cell Signaling Technology Inc drugs rabbit anti stargazin antibody
Figure 5. Junction elongation induces Rho flares. (A) Graph showing that calcium flash (GCaMP6m) follows junction elongation (verted-to-vertex length) and precedes Rho-mediated junction contraction and stabilization of length. Time 0 s represents start of Rho flare. Shaded region represents SEM; n = 16 flares, 13 embryos, 10 experiments. (B) Schematic of mosaic injection and regional activation of Rho-mediated contractility using optogenetics. Top: Schematic showing the mosaic injection of optogenetic constructs (prGEF and <t>LOVpep)</t> injected into one cell, while active Rho probe is injected in both cells at two-cell stage. Bottom: Regional activation of RhoA using optogenetics in region of stimulation (shaded gray box) and quantification of junction length and frequency of Rho flares in region of observation (dotted blue box). (C) Cell view of an embryo mosaically-expressing prGEF (2xPDZ-YFP-LARG(DH), yellow) and active Rho probe (mCherry-2xrGBD, gray). (C9) Cell view of Rho probe in the embryo from C. Regional stimulation (white dashed box) induces Rho flares in areas neighboring the region of stimulation (yellow arrowheads). (D) Quantification of Rho flares in the area outside the region of stimulation shown in C9. Frequency of Rho flares occurring pre-stimulation (0–300 s) and post-stimulation (600–900 s) are matched for color and shape. Significance calculated using unpaired two-tailed t test; n = 6 embryos, 4 experiments. (E) Time-lapse montage of a junction highlighted in C9 (yellow box) expressing active Rho probe (mCherry- 2xrGBD). Following optogenetic stimulation (indicated by gray shaded box), an increase in junction length precedes Rho flare activation (yellow arrowhead). Junction length measured from vertex to vertex is indicated under each panel.
Drugs Rabbit Anti Stargazin Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stargazin/Stargazin+Rabbit+mAb/ppr0412723-59-2-7
Average 93 stars, based on 1 article reviews
drugs rabbit anti stargazin antibody - by Bioz Stars, 2026-10
93/100 stars
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γ2 1 1000 1505 star phosphosolutions  (PhosphoSolutions)
96
PhosphoSolutions γ2 1 1000 1505 star phosphosolutions
Figure 5. Junction elongation induces Rho flares. (A) Graph showing that calcium flash (GCaMP6m) follows junction elongation (verted-to-vertex length) and precedes Rho-mediated junction contraction and stabilization of length. Time 0 s represents start of Rho flare. Shaded region represents SEM; n = 16 flares, 13 embryos, 10 experiments. (B) Schematic of mosaic injection and regional activation of Rho-mediated contractility using optogenetics. Top: Schematic showing the mosaic injection of optogenetic constructs (prGEF and <t>LOVpep)</t> injected into one cell, while active Rho probe is injected in both cells at two-cell stage. Bottom: Regional activation of RhoA using optogenetics in region of stimulation (shaded gray box) and quantification of junction length and frequency of Rho flares in region of observation (dotted blue box). (C) Cell view of an embryo mosaically-expressing prGEF (2xPDZ-YFP-LARG(DH), yellow) and active Rho probe (mCherry-2xrGBD, gray). (C9) Cell view of Rho probe in the embryo from C. Regional stimulation (white dashed box) induces Rho flares in areas neighboring the region of stimulation (yellow arrowheads). (D) Quantification of Rho flares in the area outside the region of stimulation shown in C9. Frequency of Rho flares occurring pre-stimulation (0–300 s) and post-stimulation (600–900 s) are matched for color and shape. Significance calculated using unpaired two-tailed t test; n = 6 embryos, 4 experiments. (E) Time-lapse montage of a junction highlighted in C9 (yellow box) expressing active Rho probe (mCherry- 2xrGBD). Following optogenetic stimulation (indicated by gray shaded box), an increase in junction length precedes Rho flare activation (yellow arrowhead). Junction length measured from vertex to vertex is indicated under each panel.
γ2 1 1000 1505 Star Phosphosolutions, supplied by PhosphoSolutions, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stargazin/Anti-Stargazin+Antibody/pmc03094740-114-86-89
Average 96 stars, based on 1 article reviews
γ2 1 1000 1505 star phosphosolutions - by Bioz Stars, 2026-10
96/100 stars
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guinea pig anti stargazin antibody  (Alomone Labs)
92
Alomone Labs guinea pig anti stargazin antibody
Figure 5. Junction elongation induces Rho flares. (A) Graph showing that calcium flash (GCaMP6m) follows junction elongation (verted-to-vertex length) and precedes Rho-mediated junction contraction and stabilization of length. Time 0 s represents start of Rho flare. Shaded region represents SEM; n = 16 flares, 13 embryos, 10 experiments. (B) Schematic of mosaic injection and regional activation of Rho-mediated contractility using optogenetics. Top: Schematic showing the mosaic injection of optogenetic constructs (prGEF and <t>LOVpep)</t> injected into one cell, while active Rho probe is injected in both cells at two-cell stage. Bottom: Regional activation of RhoA using optogenetics in region of stimulation (shaded gray box) and quantification of junction length and frequency of Rho flares in region of observation (dotted blue box). (C) Cell view of an embryo mosaically-expressing prGEF (2xPDZ-YFP-LARG(DH), yellow) and active Rho probe (mCherry-2xrGBD, gray). (C9) Cell view of Rho probe in the embryo from C. Regional stimulation (white dashed box) induces Rho flares in areas neighboring the region of stimulation (yellow arrowheads). (D) Quantification of Rho flares in the area outside the region of stimulation shown in C9. Frequency of Rho flares occurring pre-stimulation (0–300 s) and post-stimulation (600–900 s) are matched for color and shape. Significance calculated using unpaired two-tailed t test; n = 6 embryos, 4 experiments. (E) Time-lapse montage of a junction highlighted in C9 (yellow box) expressing active Rho probe (mCherry- 2xrGBD). Following optogenetic stimulation (indicated by gray shaded box), an increase in junction length precedes Rho flare activation (yellow arrowhead). Junction length measured from vertex to vertex is indicated under each panel.
Guinea Pig Anti Stargazin Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stargazin/Guinea+pig+Anti-Stargazin+Antibody/bio_rxiv__2024__08__04__606518-192-5-10
Average 92 stars, based on 1 article reviews
guinea pig anti stargazin antibody - by Bioz Stars, 2026-10
92/100 stars
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p-s6 (ser-240/244; #07-2113)  (Merck KGaA)
90
Merck KGaA p-s6 (ser-240/244; #07-2113)
Figure 5. Junction elongation induces Rho flares. (A) Graph showing that calcium flash (GCaMP6m) follows junction elongation (verted-to-vertex length) and precedes Rho-mediated junction contraction and stabilization of length. Time 0 s represents start of Rho flare. Shaded region represents SEM; n = 16 flares, 13 embryos, 10 experiments. (B) Schematic of mosaic injection and regional activation of Rho-mediated contractility using optogenetics. Top: Schematic showing the mosaic injection of optogenetic constructs (prGEF and <t>LOVpep)</t> injected into one cell, while active Rho probe is injected in both cells at two-cell stage. Bottom: Regional activation of RhoA using optogenetics in region of stimulation (shaded gray box) and quantification of junction length and frequency of Rho flares in region of observation (dotted blue box). (C) Cell view of an embryo mosaically-expressing prGEF (2xPDZ-YFP-LARG(DH), yellow) and active Rho probe (mCherry-2xrGBD, gray). (C9) Cell view of Rho probe in the embryo from C. Regional stimulation (white dashed box) induces Rho flares in areas neighboring the region of stimulation (yellow arrowheads). (D) Quantification of Rho flares in the area outside the region of stimulation shown in C9. Frequency of Rho flares occurring pre-stimulation (0–300 s) and post-stimulation (600–900 s) are matched for color and shape. Significance calculated using unpaired two-tailed t test; n = 6 embryos, 4 experiments. (E) Time-lapse montage of a junction highlighted in C9 (yellow box) expressing active Rho probe (mCherry- 2xrGBD). Following optogenetic stimulation (indicated by gray shaded box), an increase in junction length precedes Rho flare activation (yellow arrowhead). Junction length measured from vertex to vertex is indicated under each panel.
P S6 (Ser 240/244; #07 2113), supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stargazin/anti+phospho+stargazin++ser239+ser240++ab3713/pmc08073773-64-5-10
Average 90 stars, based on 1 article reviews
p-s6 (ser-240/244; #07-2113) - by Bioz Stars, 2026-10
90/100 stars
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anti-stargazin specific antibody  (Immune Systems Ltd)
90
Immune Systems Ltd anti-stargazin specific antibody
Figure 5. Junction elongation induces Rho flares. (A) Graph showing that calcium flash (GCaMP6m) follows junction elongation (verted-to-vertex length) and precedes Rho-mediated junction contraction and stabilization of length. Time 0 s represents start of Rho flare. Shaded region represents SEM; n = 16 flares, 13 embryos, 10 experiments. (B) Schematic of mosaic injection and regional activation of Rho-mediated contractility using optogenetics. Top: Schematic showing the mosaic injection of optogenetic constructs (prGEF and <t>LOVpep)</t> injected into one cell, while active Rho probe is injected in both cells at two-cell stage. Bottom: Regional activation of RhoA using optogenetics in region of stimulation (shaded gray box) and quantification of junction length and frequency of Rho flares in region of observation (dotted blue box). (C) Cell view of an embryo mosaically-expressing prGEF (2xPDZ-YFP-LARG(DH), yellow) and active Rho probe (mCherry-2xrGBD, gray). (C9) Cell view of Rho probe in the embryo from C. Regional stimulation (white dashed box) induces Rho flares in areas neighboring the region of stimulation (yellow arrowheads). (D) Quantification of Rho flares in the area outside the region of stimulation shown in C9. Frequency of Rho flares occurring pre-stimulation (0–300 s) and post-stimulation (600–900 s) are matched for color and shape. Significance calculated using unpaired two-tailed t test; n = 6 embryos, 4 experiments. (E) Time-lapse montage of a junction highlighted in C9 (yellow box) expressing active Rho probe (mCherry- 2xrGBD). Following optogenetic stimulation (indicated by gray shaded box), an increase in junction length precedes Rho flare activation (yellow arrowhead). Junction length measured from vertex to vertex is indicated under each panel.
Anti Stargazin Specific Antibody, supplied by Immune Systems Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stargazin/anti+stargazin+specific+antibody/10__1074_slash_jbc__m402214200-74-2-15
Average 90 stars, based on 1 article reviews
anti-stargazin specific antibody - by Bioz Stars, 2026-10
90/100 stars
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stargazin tarp  (Bedoukian Research)
90
Bedoukian Research stargazin tarp
Figure 5. Junction elongation induces Rho flares. (A) Graph showing that calcium flash (GCaMP6m) follows junction elongation (verted-to-vertex length) and precedes Rho-mediated junction contraction and stabilization of length. Time 0 s represents start of Rho flare. Shaded region represents SEM; n = 16 flares, 13 embryos, 10 experiments. (B) Schematic of mosaic injection and regional activation of Rho-mediated contractility using optogenetics. Top: Schematic showing the mosaic injection of optogenetic constructs (prGEF and <t>LOVpep)</t> injected into one cell, while active Rho probe is injected in both cells at two-cell stage. Bottom: Regional activation of RhoA using optogenetics in region of stimulation (shaded gray box) and quantification of junction length and frequency of Rho flares in region of observation (dotted blue box). (C) Cell view of an embryo mosaically-expressing prGEF (2xPDZ-YFP-LARG(DH), yellow) and active Rho probe (mCherry-2xrGBD, gray). (C9) Cell view of Rho probe in the embryo from C. Regional stimulation (white dashed box) induces Rho flares in areas neighboring the region of stimulation (yellow arrowheads). (D) Quantification of Rho flares in the area outside the region of stimulation shown in C9. Frequency of Rho flares occurring pre-stimulation (0–300 s) and post-stimulation (600–900 s) are matched for color and shape. Significance calculated using unpaired two-tailed t test; n = 6 embryos, 4 experiments. (E) Time-lapse montage of a junction highlighted in C9 (yellow box) expressing active Rho probe (mCherry- 2xrGBD). Following optogenetic stimulation (indicated by gray shaded box), an increase in junction length precedes Rho flare activation (yellow arrowhead). Junction length measured from vertex to vertex is indicated under each panel.
Stargazin Tarp, supplied by Bedoukian Research, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stargazin/stargazin+tarp/pm18817736-157-4-17
Average 90 stars, based on 1 article reviews
stargazin tarp - by Bioz Stars, 2026-10
90/100 stars
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ligand derived from stargazin  (BioMimetic Therapeutics)
90
BioMimetic Therapeutics ligand derived from stargazin
Figure 5. Junction elongation induces Rho flares. (A) Graph showing that calcium flash (GCaMP6m) follows junction elongation (verted-to-vertex length) and precedes Rho-mediated junction contraction and stabilization of length. Time 0 s represents start of Rho flare. Shaded region represents SEM; n = 16 flares, 13 embryos, 10 experiments. (B) Schematic of mosaic injection and regional activation of Rho-mediated contractility using optogenetics. Top: Schematic showing the mosaic injection of optogenetic constructs (prGEF and <t>LOVpep)</t> injected into one cell, while active Rho probe is injected in both cells at two-cell stage. Bottom: Regional activation of RhoA using optogenetics in region of stimulation (shaded gray box) and quantification of junction length and frequency of Rho flares in region of observation (dotted blue box). (C) Cell view of an embryo mosaically-expressing prGEF (2xPDZ-YFP-LARG(DH), yellow) and active Rho probe (mCherry-2xrGBD, gray). (C9) Cell view of Rho probe in the embryo from C. Regional stimulation (white dashed box) induces Rho flares in areas neighboring the region of stimulation (yellow arrowheads). (D) Quantification of Rho flares in the area outside the region of stimulation shown in C9. Frequency of Rho flares occurring pre-stimulation (0–300 s) and post-stimulation (600–900 s) are matched for color and shape. Significance calculated using unpaired two-tailed t test; n = 6 embryos, 4 experiments. (E) Time-lapse montage of a junction highlighted in C9 (yellow box) expressing active Rho probe (mCherry- 2xrGBD). Following optogenetic stimulation (indicated by gray shaded box), an increase in junction length precedes Rho flare activation (yellow arrowhead). Junction length measured from vertex to vertex is indicated under each panel.
Ligand Derived From Stargazin, supplied by BioMimetic Therapeutics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stargazin/ligand+derived+from+stargazin/pmc05435282-233-16-12
Average 90 stars, based on 1 article reviews
ligand derived from stargazin - by Bioz Stars, 2026-10
90/100 stars
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26-amino-acid linker followed stargazin 13 last amino acids  (GenScript corporation)
90
GenScript corporation 26-amino-acid linker followed stargazin 13 last amino acids
Figure 5. Junction elongation induces Rho flares. (A) Graph showing that calcium flash (GCaMP6m) follows junction elongation (verted-to-vertex length) and precedes Rho-mediated junction contraction and stabilization of length. Time 0 s represents start of Rho flare. Shaded region represents SEM; n = 16 flares, 13 embryos, 10 experiments. (B) Schematic of mosaic injection and regional activation of Rho-mediated contractility using optogenetics. Top: Schematic showing the mosaic injection of optogenetic constructs (prGEF and <t>LOVpep)</t> injected into one cell, while active Rho probe is injected in both cells at two-cell stage. Bottom: Regional activation of RhoA using optogenetics in region of stimulation (shaded gray box) and quantification of junction length and frequency of Rho flares in region of observation (dotted blue box). (C) Cell view of an embryo mosaically-expressing prGEF (2xPDZ-YFP-LARG(DH), yellow) and active Rho probe (mCherry-2xrGBD, gray). (C9) Cell view of Rho probe in the embryo from C. Regional stimulation (white dashed box) induces Rho flares in areas neighboring the region of stimulation (yellow arrowheads). (D) Quantification of Rho flares in the area outside the region of stimulation shown in C9. Frequency of Rho flares occurring pre-stimulation (0–300 s) and post-stimulation (600–900 s) are matched for color and shape. Significance calculated using unpaired two-tailed t test; n = 6 embryos, 4 experiments. (E) Time-lapse montage of a junction highlighted in C9 (yellow box) expressing active Rho probe (mCherry- 2xrGBD). Following optogenetic stimulation (indicated by gray shaded box), an increase in junction length precedes Rho flare activation (yellow arrowhead). Junction length measured from vertex to vertex is indicated under each panel.
26 Amino Acid Linker Followed Stargazin 13 Last Amino Acids, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stargazin/26+amino+acid+linker+followed+stargazin+13+last+amino+acids/pmc06778148-325-5-16
Average 90 stars, based on 1 article reviews
26-amino-acid linker followed stargazin 13 last amino acids - by Bioz Stars, 2026-10
90/100 stars
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anti-phospho stargazin (ser228) ab15435  (Merck KGaA)
90
Merck KGaA anti-phospho stargazin (ser228) ab15435
Figure 5. Junction elongation induces Rho flares. (A) Graph showing that calcium flash (GCaMP6m) follows junction elongation (verted-to-vertex length) and precedes Rho-mediated junction contraction and stabilization of length. Time 0 s represents start of Rho flare. Shaded region represents SEM; n = 16 flares, 13 embryos, 10 experiments. (B) Schematic of mosaic injection and regional activation of Rho-mediated contractility using optogenetics. Top: Schematic showing the mosaic injection of optogenetic constructs (prGEF and <t>LOVpep)</t> injected into one cell, while active Rho probe is injected in both cells at two-cell stage. Bottom: Regional activation of RhoA using optogenetics in region of stimulation (shaded gray box) and quantification of junction length and frequency of Rho flares in region of observation (dotted blue box). (C) Cell view of an embryo mosaically-expressing prGEF (2xPDZ-YFP-LARG(DH), yellow) and active Rho probe (mCherry-2xrGBD, gray). (C9) Cell view of Rho probe in the embryo from C. Regional stimulation (white dashed box) induces Rho flares in areas neighboring the region of stimulation (yellow arrowheads). (D) Quantification of Rho flares in the area outside the region of stimulation shown in C9. Frequency of Rho flares occurring pre-stimulation (0–300 s) and post-stimulation (600–900 s) are matched for color and shape. Significance calculated using unpaired two-tailed t test; n = 6 embryos, 4 experiments. (E) Time-lapse montage of a junction highlighted in C9 (yellow box) expressing active Rho probe (mCherry- 2xrGBD). Following optogenetic stimulation (indicated by gray shaded box), an increase in junction length precedes Rho flare activation (yellow arrowhead). Junction length measured from vertex to vertex is indicated under each panel.
Anti Phospho Stargazin (Ser228) Ab15435, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stargazin/anti+phospho+stargazin++ser228++ab15435/pmc04115130-350-13-2
Average 90 stars, based on 1 article reviews
anti-phospho stargazin (ser228) ab15435 - by Bioz Stars, 2026-10
90/100 stars
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stargazin ctd  (Bedoukian Research)
90
Bedoukian Research stargazin ctd
Figure 5. Junction elongation induces Rho flares. (A) Graph showing that calcium flash (GCaMP6m) follows junction elongation (verted-to-vertex length) and precedes Rho-mediated junction contraction and stabilization of length. Time 0 s represents start of Rho flare. Shaded region represents SEM; n = 16 flares, 13 embryos, 10 experiments. (B) Schematic of mosaic injection and regional activation of Rho-mediated contractility using optogenetics. Top: Schematic showing the mosaic injection of optogenetic constructs (prGEF and <t>LOVpep)</t> injected into one cell, while active Rho probe is injected in both cells at two-cell stage. Bottom: Regional activation of RhoA using optogenetics in region of stimulation (shaded gray box) and quantification of junction length and frequency of Rho flares in region of observation (dotted blue box). (C) Cell view of an embryo mosaically-expressing prGEF (2xPDZ-YFP-LARG(DH), yellow) and active Rho probe (mCherry-2xrGBD, gray). (C9) Cell view of Rho probe in the embryo from C. Regional stimulation (white dashed box) induces Rho flares in areas neighboring the region of stimulation (yellow arrowheads). (D) Quantification of Rho flares in the area outside the region of stimulation shown in C9. Frequency of Rho flares occurring pre-stimulation (0–300 s) and post-stimulation (600–900 s) are matched for color and shape. Significance calculated using unpaired two-tailed t test; n = 6 embryos, 4 experiments. (E) Time-lapse montage of a junction highlighted in C9 (yellow box) expressing active Rho probe (mCherry- 2xrGBD). Following optogenetic stimulation (indicated by gray shaded box), an increase in junction length precedes Rho flare activation (yellow arrowhead). Junction length measured from vertex to vertex is indicated under each panel.
Stargazin Ctd, supplied by Bedoukian Research, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stargazin/stargazin+ctd/pmc03119519-76-2-29
Average 90 stars, based on 1 article reviews
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Image Search Results


Numbers of primary antibodies per synaptosome for over 100 targets.

Journal: Scientific Reports

Article Title: Comparative synaptosome imaging: a semi-quantitative method to obtain copy numbers for synaptic and neuronal proteins

doi: 10.1038/s41598-018-33130-6

Figure Lengend Snippet: Numbers of primary antibodies per synaptosome for over 100 targets.

Article Snippet: Stargazin , 5.314 , 0.296 (SEM) , 143.78 , 12.33 , Alomone , ACC-012 , rabbit polyclonal , 1:200.

Techniques:

Figure 5. Junction elongation induces Rho flares. (A) Graph showing that calcium flash (GCaMP6m) follows junction elongation (verted-to-vertex length) and precedes Rho-mediated junction contraction and stabilization of length. Time 0 s represents start of Rho flare. Shaded region represents SEM; n = 16 flares, 13 embryos, 10 experiments. (B) Schematic of mosaic injection and regional activation of Rho-mediated contractility using optogenetics. Top: Schematic showing the mosaic injection of optogenetic constructs (prGEF and LOVpep) injected into one cell, while active Rho probe is injected in both cells at two-cell stage. Bottom: Regional activation of RhoA using optogenetics in region of stimulation (shaded gray box) and quantification of junction length and frequency of Rho flares in region of observation (dotted blue box). (C) Cell view of an embryo mosaically-expressing prGEF (2xPDZ-YFP-LARG(DH), yellow) and active Rho probe (mCherry-2xrGBD, gray). (C9) Cell view of Rho probe in the embryo from C. Regional stimulation (white dashed box) induces Rho flares in areas neighboring the region of stimulation (yellow arrowheads). (D) Quantification of Rho flares in the area outside the region of stimulation shown in C9. Frequency of Rho flares occurring pre-stimulation (0–300 s) and post-stimulation (600–900 s) are matched for color and shape. Significance calculated using unpaired two-tailed t test; n = 6 embryos, 4 experiments. (E) Time-lapse montage of a junction highlighted in C9 (yellow box) expressing active Rho probe (mCherry- 2xrGBD). Following optogenetic stimulation (indicated by gray shaded box), an increase in junction length precedes Rho flare activation (yellow arrowhead). Junction length measured from vertex to vertex is indicated under each panel.

Journal: The Journal of cell biology

Article Title: Mechanosensitive calcium flashes promote sustained RhoA activation during tight junction remodeling.

doi: 10.1083/jcb.202105107

Figure Lengend Snippet: Figure 5. Junction elongation induces Rho flares. (A) Graph showing that calcium flash (GCaMP6m) follows junction elongation (verted-to-vertex length) and precedes Rho-mediated junction contraction and stabilization of length. Time 0 s represents start of Rho flare. Shaded region represents SEM; n = 16 flares, 13 embryos, 10 experiments. (B) Schematic of mosaic injection and regional activation of Rho-mediated contractility using optogenetics. Top: Schematic showing the mosaic injection of optogenetic constructs (prGEF and LOVpep) injected into one cell, while active Rho probe is injected in both cells at two-cell stage. Bottom: Regional activation of RhoA using optogenetics in region of stimulation (shaded gray box) and quantification of junction length and frequency of Rho flares in region of observation (dotted blue box). (C) Cell view of an embryo mosaically-expressing prGEF (2xPDZ-YFP-LARG(DH), yellow) and active Rho probe (mCherry-2xrGBD, gray). (C9) Cell view of Rho probe in the embryo from C. Regional stimulation (white dashed box) induces Rho flares in areas neighboring the region of stimulation (yellow arrowheads). (D) Quantification of Rho flares in the area outside the region of stimulation shown in C9. Frequency of Rho flares occurring pre-stimulation (0–300 s) and post-stimulation (600–900 s) are matched for color and shape. Significance calculated using unpaired two-tailed t test; n = 6 embryos, 4 experiments. (E) Time-lapse montage of a junction highlighted in C9 (yellow box) expressing active Rho probe (mCherry- 2xrGBD). Following optogenetic stimulation (indicated by gray shaded box), an increase in junction length precedes Rho flare activation (yellow arrowhead). Junction length measured from vertex to vertex is indicated under each panel.

Article Snippet: Stargazin-GFP-LOVpep (referred to as GFPLOVpep) was generated by PCR amplifying the Stargazin-GFP- LOVpep sequence (Addgene plasmid #80406; Wagner and Glotzer, 2016) and ligating into the ClaI site in pCS2+.

Techniques: Injection, Activation Assay, Optogenetics, Construct, Expressing, Two Tailed Test