Journal: FEBS letters

Article Title: Molecular mechanism of molt-inhibiting hormone (MIH) induced suppression of ecdysteroidogenesis in the Y-organ of mud crab: Scylla serrata.

doi: 10.1016/j.febslet.2007.09.029

Figure Lengend Snippet: Fig. 1. (a) A control section of eyestalk, no immunoreactivity of MIH was observed. (b) Represents whole structure of eyestalk, showing three distinct structures of medulla externa, medulla interna and medulla terminals. Medulla interna and terminals showing higher immunopositive reaction of MIH protein. (c) The distribution of MIH protein on the target cell of Y-organs. Membranous expression of MIH protein on the Y-organ cells is seen (red arrow). (d) Shows the membranous expression of ApoB in the premolt stage of Y-organ cells. Membranous expression as well as cytosolic expression indicates that ApoB predominantly accumulated in the cytosolic region for ecdysteroid biosynthesis. red arrow indicates the counter stain with haematoxylin (blue). (e) Expression of StAR protein in the premolt stages of Y-organs cells. Results shows that cytosolic distribution of StAR is protein required for delivery of cholesterol from outer membrane to inner membrane of mitochondria. (f) Represents Oil Red ‘O’ staining of cholesterol in the Y-organ cells. cholesterol is predominantly distributed in the cytosolic region of Y-organ of premolt stages.

Article Snippet: The following antibodies were used: MIH (1:8000), StAR (1:10000), phospho ERK (1:10000) (Santa Cruz Biotechnology) and d protein kinase C (1:10000) (Santa Cruz Biotechnology).

Techniques: Control, Expressing, Staining, Membrane

Journal: FEBS letters

Article Title: Molecular mechanism of molt-inhibiting hormone (MIH) induced suppression of ecdysteroidogenesis in the Y-organ of mud crab: Scylla serrata.

doi: 10.1016/j.febslet.2007.09.029

Figure Lengend Snippet: Fig. 2. (a) The expression of b actin in all molting stages as positive control. (b) The expression of MIH in the X-organ sinus gland complex of eyestalk. Approximately 8–9 kDa of MIH is expressed in all molting stages except in early and middle premolt stages (D0 and D1), but intense expression was observed only in the intermolt stage. (c) The phosphorylation of ERK was found only in early postmolt (A), middle postmolt (B) and intermolt (C) stages but no phosphorylation of ERK was found in the premolt stages. (d) Shows that the expression of Protein kinase C d was seen only in the premolt stages such as early premolt (D0), middle premolt (D1) and late premolt (D2–3). No expression of PKC was found in postmolt (A and B) and intermolt (C) stages. (e) The expression of StAR protein in the premolt stage of Y- organ. Expression of StAR protein was seen predominantly in the early premolt (D0), middle premolt (D1) and late premolt (D2–3) stages. Very little expression was found in early post molt stages (A).

Article Snippet: The following antibodies were used: MIH (1:8000), StAR (1:10000), phospho ERK (1:10000) (Santa Cruz Biotechnology) and d protein kinase C (1:10000) (Santa Cruz Biotechnology).

Techniques: Expressing, Positive Control, Phospho-proteomics

Journal: FEBS letters

Article Title: Molecular mechanism of molt-inhibiting hormone (MIH) induced suppression of ecdysteroidogenesis in the Y-organ of mud crab: Scylla serrata.

doi: 10.1016/j.febslet.2007.09.029

Figure Lengend Snippet: Fig. 3. (a) The effect of PD98059 on the phosphoylation of ERK in the Y-organs. No phosphorylation of ERK was found in the PD98059 treated stages. (b) Shows the expression of Protein kinase C d in the PD98059 treated stages – early post molt (A), middle post molt (B) and intermolt stages (C). (c) Expression of StAR protein in the PD98059 treated stages – middle post molt (B) and intermolt (C) stages.

Article Snippet: The following antibodies were used: MIH (1:8000), StAR (1:10000), phospho ERK (1:10000) (Santa Cruz Biotechnology) and d protein kinase C (1:10000) (Santa Cruz Biotechnology).

Techniques: Phospho-proteomics, Expressing

Journal: FEBS letters

Article Title: Molecular mechanism of molt-inhibiting hormone (MIH) induced suppression of ecdysteroidogenesis in the Y-organ of mud crab: Scylla serrata.

doi: 10.1016/j.febslet.2007.09.029

Figure Lengend Snippet: Fig. 5. MIH induced ERK activation via Ras, Raf pathway involving the suppression of ecdysteroidogenesis and PKC mediated StAR transcription.

Article Snippet: The following antibodies were used: MIH (1:8000), StAR (1:10000), phospho ERK (1:10000) (Santa Cruz Biotechnology) and d protein kinase C (1:10000) (Santa Cruz Biotechnology).

Techniques: Activation Assay