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Image Search Results
Journal: Applied and Environmental Microbiology
Article Title: Quantitative metagenomics using a portable protocol
doi: 10.1128/aem.02179-25
Figure Lengend Snippet: Evaluation of DNA extraction methods on the microbial community in mixed liquor sludge samples (ML). ( A ) Purity and quantity and ( B ) DNA fragment size distribution for DNA extracted using different protocols. The line, bar, and bubble plots indicate the average purity, DNA yield, and fragment distribution, respectively. The star, circle, and filled triangle represent the mean, median, and peak of DNA fragments by molarity, respectively. ( C ) Alpha diversity (Chao1 index), ( D ) beta diversity, and ( E ) composition of the microbial community for prokaryotes using 16S rRNA gene amplicon sequencing. Beta diversity is measured using an NMDS2 plot based on Bray-Curtis distance.
Article Snippet: DNA extracted from ZymoBIOMICS Microbial Community Standard (Even Distribution) (Zymo Even Cell, ) and
Techniques: DNA Extraction, Amplification, Sequencing
Journal: Journal of Biological Chemistry
Article Title: Smac/DIABLO Selectively Reduces the Levels of c-IAP1 and c-IAP2 but Not That of XIAP and Livin in HeLa Cells
doi: 10.1074/jbc.m401253200
Figure Lengend Snippet: FIG. 6. Smac does not promote the auto-ubiquitination of XIAP in vitro. The purified XIAP (without tag) was incubated for 2 h at 30 °C with or without different concentrations of Smac or with Smac A in the reconstituted ubiquitination reaction system (Ub Mix) consisting of 50 mM Tris-HCl (pH 7.5), 50 mM NaCl, 2 mM Mg-ATP, 20 M mammalian ubiquitin, 100 nM rabbit E1, and 400 nM UbcH6 (E2). The reactions were stopped by adding equal volumes of 2 SDS sample loading buffer, and the products were subjected to SDS-PAGE followed by immunoblotting with anti-XIAP antibody.
Article Snippet: This paper is available on line at http://www.jbc.org 16963 at SO U T H E R N IL L IN O IS U N IV on M arch 5, 2015 http://w w w .jbc.org/ D ow nloaded from of human c-IAP2, and residues 244–263 of
Techniques: Ubiquitin Proteomics, In Vitro, Purification, Incubation, SDS Page, Western Blot