standards Search Results


onetaq master mix  (New England Biolabs)
96
New England Biolabs onetaq master mix
Onetaq Master Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vectastain abc elite standard kit  (Vector Laboratories)
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Vector Laboratories vectastain abc elite standard kit
Vectastain Abc Elite Standard Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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taq dna polymerase  (New England Biolabs)
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New England Biolabs taq dna polymerase
Taq Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bio rad protein assay  (Bio-Rad)
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Bio-Rad bio rad protein assay
Bio Rad Protein Assay, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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prepacked gel filtration column  (Bio-Rad)
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Bio-Rad prepacked gel filtration column
Prepacked Gel Filtration Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cuvette  (Bio-Rad)
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Bio-Rad cuvette
Cuvette, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vectastain abc peroxidase kit  (Vector Laboratories)
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Vector Laboratories vectastain abc peroxidase kit
Vectastain Abc Peroxidase Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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žthe vectastain abc kit vector laboratories  (Vector Laboratories)
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Vector Laboratories žthe vectastain abc kit vector laboratories
žthe Vectastain Abc Kit Vector Laboratories, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ief standards  (Bio-Rad)
99
Bio-Rad ief standards
FIG. 1. Electrophoretic analysis of NGF and PUT-NGF modified at different pH values. A: SOS-PAGE (15% T, 1% C) of Coomas- sie Blue-stained gel. Native NGF shows a molecular weight of the monomer at 13,000 and the dimer at 26,000. NGFwas modi- fied with PUT at pH values of 5.7, 6.0, 6.4, and 6.7 and revealed the shifted molecular weight of the monomeric species and the presence of two new species at ‘—18,000 and ‘--28,000. All pro- teins were electrophoresed at 10 jig/lane. B: Western blot analy- sis after SDS-PAGE comparing immunoreactivity of native NGF versus NGF modified with PUT at pH 5.7, 6.0, 6.4, and 6.7 (1 jig of protein/lane). C: <t>IEF</t> analysis following Coomassie Blue stain. Native NGF shows a p1 of 8.0, and PUT-NGF shows a p1 of 9.6 after modification with PUT at the different pH values. In addition to the standards, the p1 values of cytochrome C (CYT C) and albumin (ALB) are also shown.
Ief Standards, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bovine serum albumin  (Bio-Rad)
96
Bio-Rad bovine serum albumin
FIG. 1. Electrophoretic analysis of NGF and PUT-NGF modified at different pH values. A: SOS-PAGE (15% T, 1% C) of Coomas- sie Blue-stained gel. Native NGF shows a molecular weight of the monomer at 13,000 and the dimer at 26,000. NGFwas modi- fied with PUT at pH values of 5.7, 6.0, 6.4, and 6.7 and revealed the shifted molecular weight of the monomeric species and the presence of two new species at ‘—18,000 and ‘--28,000. All pro- teins were electrophoresed at 10 jig/lane. B: Western blot analy- sis after SDS-PAGE comparing immunoreactivity of native NGF versus NGF modified with PUT at pH 5.7, 6.0, 6.4, and 6.7 (1 jig of protein/lane). C: <t>IEF</t> analysis following Coomassie Blue stain. Native NGF shows a p1 of 8.0, and PUT-NGF shows a p1 of 9.6 after modification with PUT at the different pH values. In addition to the standards, the p1 values of cytochrome C (CYT C) and albumin (ALB) are also shown.
Bovine Serum Albumin, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sds page standards  (Bio-Rad)
96
Bio-Rad sds page standards
FIG. 1. Electrophoretic analysis of NGF and PUT-NGF modified at different pH values. A: SOS-PAGE (15% T, 1% C) of Coomas- sie Blue-stained gel. Native NGF shows a molecular weight of the monomer at 13,000 and the dimer at 26,000. NGFwas modi- fied with PUT at pH values of 5.7, 6.0, 6.4, and 6.7 and revealed the shifted molecular weight of the monomeric species and the presence of two new species at ‘—18,000 and ‘--28,000. All pro- teins were electrophoresed at 10 jig/lane. B: Western blot analy- sis after SDS-PAGE comparing immunoreactivity of native NGF versus NGF modified with PUT at pH 5.7, 6.0, 6.4, and 6.7 (1 jig of protein/lane). C: <t>IEF</t> analysis following Coomassie Blue stain. Native NGF shows a p1 of 8.0, and PUT-NGF shows a p1 of 9.6 after modification with PUT at the different pH values. In addition to the standards, the p1 values of cytochrome C (CYT C) and albumin (ALB) are also shown.
Sds Page Standards, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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zymo mock community  (Zymo Research)
99
Zymo Research zymo mock community
FIG. 1. Electrophoretic analysis of NGF and PUT-NGF modified at different pH values. A: SOS-PAGE (15% T, 1% C) of Coomas- sie Blue-stained gel. Native NGF shows a molecular weight of the monomer at 13,000 and the dimer at 26,000. NGFwas modi- fied with PUT at pH values of 5.7, 6.0, 6.4, and 6.7 and revealed the shifted molecular weight of the monomeric species and the presence of two new species at ‘—18,000 and ‘--28,000. All pro- teins were electrophoresed at 10 jig/lane. B: Western blot analy- sis after SDS-PAGE comparing immunoreactivity of native NGF versus NGF modified with PUT at pH 5.7, 6.0, 6.4, and 6.7 (1 jig of protein/lane). C: <t>IEF</t> analysis following Coomassie Blue stain. Native NGF shows a p1 of 8.0, and PUT-NGF shows a p1 of 9.6 after modification with PUT at the different pH values. In addition to the standards, the p1 values of cytochrome C (CYT C) and albumin (ALB) are also shown.
Zymo Mock Community, supplied by Zymo Research, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


FIG. 1. Electrophoretic analysis of NGF and PUT-NGF modified at different pH values. A: SOS-PAGE (15% T, 1% C) of Coomas- sie Blue-stained gel. Native NGF shows a molecular weight of the monomer at 13,000 and the dimer at 26,000. NGFwas modi- fied with PUT at pH values of 5.7, 6.0, 6.4, and 6.7 and revealed the shifted molecular weight of the monomeric species and the presence of two new species at ‘—18,000 and ‘--28,000. All pro- teins were electrophoresed at 10 jig/lane. B: Western blot analy- sis after SDS-PAGE comparing immunoreactivity of native NGF versus NGF modified with PUT at pH 5.7, 6.0, 6.4, and 6.7 (1 jig of protein/lane). C: IEF analysis following Coomassie Blue stain. Native NGF shows a p1 of 8.0, and PUT-NGF shows a p1 of 9.6 after modification with PUT at the different pH values. In addition to the standards, the p1 values of cytochrome C (CYT C) and albumin (ALB) are also shown.

Journal: Journal of neurochemistry

Article Title: Putrescine-modified nerve growth factor: bioactivity, plasma pharmacokinetics, blood-brain/nerve barrier permeability, and nervous system biodistribution.

doi: 10.1046/j.1471-4159.1998.71041651.x

Figure Lengend Snippet: FIG. 1. Electrophoretic analysis of NGF and PUT-NGF modified at different pH values. A: SOS-PAGE (15% T, 1% C) of Coomas- sie Blue-stained gel. Native NGF shows a molecular weight of the monomer at 13,000 and the dimer at 26,000. NGFwas modi- fied with PUT at pH values of 5.7, 6.0, 6.4, and 6.7 and revealed the shifted molecular weight of the monomeric species and the presence of two new species at ‘—18,000 and ‘--28,000. All pro- teins were electrophoresed at 10 jig/lane. B: Western blot analy- sis after SDS-PAGE comparing immunoreactivity of native NGF versus NGF modified with PUT at pH 5.7, 6.0, 6.4, and 6.7 (1 jig of protein/lane). C: IEF analysis following Coomassie Blue stain. Native NGF shows a p1 of 8.0, and PUT-NGF shows a p1 of 9.6 after modification with PUT at the different pH values. In addition to the standards, the p1 values of cytochrome C (CYT C) and albumin (ALB) are also shown.

Article Snippet: IEF standards were obtained from Bio-Rad; they contained nine proteins with isoelectric points ranging from 4.45 to 9.6 and were visualized with Coomassie Blue.

Techniques: Modification, Staining, Molecular Weight, Western Blot, SDS Page