Journal: Frontiers in Pharmacology

Article Title: Characterization of lysine crotonylation-related lncRNAs for prognostic assessment and immune response in glioma

doi: 10.3389/fphar.2025.1573694

Figure Lengend Snippet: Experimental validation of POLR2J4 function in glioma drug resistance and tumor progression. (A) RT-qPCR confirming knockdown efficiency of POLR2J4 in U87 and U251 cells using three shRNAs. (B) Dose–response curves showing increased sensitivity to Cisplatin (CDDP) in POLR2J4 knockdown cells. (C) Relative cell viability in four groups after treatment with 8 μM CDDP for 48 h. (D,E) EdU staining and quantification of proliferative cells across four groups. (F–H) Transwell assays evaluating cell migration and invasion, with quantification of migrated and invaded cells. (I–K) In vivo tumor growth analysis in nude mice (sh-N.C. vs. sh-POLR2J4) using U87 cells, with representative images (I) , growth curves (J, n = 3), and final tumor weights (K) . (L) RT-qPCR analysis of chemoresistance-related genes (ABCB1, ABCC1, BCL2) in U87 cells, demonstrating significant downregulation in the POLR2J4 knockdown group. (M) Serum ELISA analysis of pro-tumorigenic cytokines IL-6 and TGF-β1 in tumor-bearing nude mice, indicating reduced circulating levels after POLR2J4 knockdown. (N,O) IHC staining and semi-quantitative analysis (IOD/mm 2 ) of TGF-β1 and PD-L1 in xenografts, indicating decreased expression in knockdown tumors. *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: The concentrations of interleukin-6 (IL-6) and transforming growth factor-β1 (TGF-β1) in the serum were measured using commercially available mouse ELISA kits (Multi Sciences Biotech, Hangzhou, China; catalog nos.

Techniques: Biomarker Discovery, Quantitative RT-PCR, Knockdown, Staining, Migration, In Vivo, Enzyme-linked Immunosorbent Assay, Immunohistochemistry, Expressing

Journal: Disease Markers

Article Title: Identification of Gingival Crevicular Fluid Sampling, Analytical Methods, and Oral Biomarkers for the Diagnosis and Monitoring of Periodontal Diseases: A Systematic Review

doi: 10.1155/2016/1804727

Figure Lengend Snippet: Comparison of the mean of IL-1 β concentration between different studies using the same analytical techniques.

Article Snippet: ELISA kit , Standard ELISA kit (Bender Med Systems, Vienna, Austria) , Standard ELISA kit (Immunotech, France) , Standard ELISA kit (RayBiotech) , Standard ELISA kit (Quantikine R & D System) , Standard ELISA kit (Biosource, Ontario, CA).

Techniques: Comparison, Concentration Assay, Enzyme-linked Immunosorbent Assay

Journal: Cell Proliferation

Article Title: Dermal papilla cells and melanocytes response to physiological oxygen levels depends on their interactions

doi: 10.1111/cpr.13013

Figure Lengend Snippet: Phenotypic assessment of co‐cultured DP cells and hMel. ELISA quantification of (A) VEGF and (B) BMP2 released by DP cells indirectly co‐cultured with hMel and the respective control conditions. Results are shown as mean ± SEM. Significant differences were analysed using an unpaired, one‐way ANOVA (n = 3) and are indicated as * P < .05; ** P < .01; *** P < .001; **** P < .0001. (C) Representative immunofluorescence images showing the expression of different DP cells and hMel markers in the native hair follicle (HF) tissue and in the DP‐HMel aggregates prepared under physoxia. Nuclei were counterstained with DAPI. White arrowheads indicate Ki67‐positive cells and dotted circles delimitate the DP spheroid area. Scale bars = 50 μm

Article Snippet: Human vascular endothelial growth factor (VEGF) ELISA Development Kit (PeproTech) and human bone morphogenetic protein‐2 (BMP2) Standard ELISA Development Kit (Petrotech) were then used following the manufacturer instructions to determine VEGF and BMP2 levels.

Techniques: Cell Culture, Enzyme-linked Immunosorbent Assay, Control, Immunofluorescence, Expressing