Journal: Frontiers in Immunology

Article Title: The Therapeutic Effect of STAT3 Signaling-Suppressed MSC on Pain and Articular Cartilage Damage in a Rat Model of Monosodium Iodoacetate-Induced Osteoarthritis

doi: 10.3389/fimmu.2018.02881

Figure Lengend Snippet: Effects of STAT3 inhibition on anti-inflammatory activity and migration potential in MSCs from osteoarthritis (OA) patients. (A) OA-MSCs were incubated with STA21 for 3 days and, ELISA was performed to measure IL-6, IL-8, IL-1β, and VEGF concentrations. (B) OA-MSCs were incubated with STA21 for 1 h. The expression of phospho (p)-STAT3 705, 727, and total STAT3 protein was analyzed by Western blotting. (C) mRNA levels for anti-inflammatory cytokines IL-10 and TGF-β were measured by real time-PCR (D) Expression of chemokine receptors in OA-MSCs incubated with STA21 was measured by real time-PCR. OA-MSC were isolated of fat tissues obtain from each 3 person with OA. Data represent the mean ± SD of 3 independent experiments ( * P < 0.05; ** p < 0.01).

Article Snippet: The cells were cultured for 4 days until 90% confluent (passage 0) and were then expanded for 2–3 passages and used for experiments. iSTAT3 OA-MSCs were prepared by treatment of OA-MSCs with 10 μM STA21 (Santa Cruz, Texas, USA) for 72 h.

Techniques: Inhibition, Activity Assay, Migration, Incubation, Enzyme-linked Immunosorbent Assay, Expressing, Western Blot, Real-time Polymerase Chain Reaction, Isolation

Journal: ACS Omega

Article Title: Application of a Novel and Sustainable Silicate Solution as an Alternative to Sodium Silicate for Clay Swelling Inhibition

doi: 10.1021/acsomega.0c01777

Figure Lengend Snippet: Mass loss (%) conducted using HP3-DSC at a rate of 10°C/min from 30 to 300 °C for AAAS silicate.

Article Snippet: The thermogravimetric analysis (TGA) of AAAS was conducted using HP3-DSC from Linseis.

Techniques:

Journal: PLoS ONE

Article Title: Characterization of Na + and Ca 2+ Channels in Zebrafish Dorsal Root Ganglion Neurons

doi: 10.1371/journal.pone.0042602

Figure Lengend Snippet: A , The current density versus test potential ( I–V ) relationship for R- and S- I Na . B , Dot plots represent the membrane capacitance of DRG neurons displaying R- (open circles) and S- I Na (filled circles). Vertical bars represent the median. *** P<0.001, Mann-Whitney U -test. C , Concentration-response curves for block of the R- and S- I Na by tetrodotoxin (TTX). Solid lines represent nonlinear regression least-square fits of experimental points to a Hill equation (see under ). D , The voltage-dependence of the Na + conductance ( G Na , left panel) and steady-state inactivation (right panel) of R- and S- I Na . Conductance was calculated from G Na = I Na /(V m −V rev ) , in which I Na is the peak current, V m is the potential of the test pulse, and V rev is the reversal potential for I Na . The solid line represents a nonlinear regression fit to the Boltzmann function: 1/(1+exp[−( V − V 1/2 )/ k ]), where V is the step membrane potential, V 1/2 is the half-activation potential, and k is a slope factor. Voltage-dependence of inactivation was determined using a 200 ms conditioning pulse followed by a test pulse to −10 mV. Test pulse currents were normalized to the maximal value. Solid line is a fit to the Boltzmann equation ( k is negative for inactivation curve). Error bars on each symbol represent the mean ± s . e . m . The number of neurons tested is shown in parentheses.

Article Snippet: Stock solutions were made for the following drugs: γ-aminobutyric acid (GABA), l -glutamic acid hydrochloride, oxotremorine M, FPL64176 (all from TOCRIS Cookson Ltd., Ellisville, MO), nifedipine (EMD chemicals, Gibbstown, NJ), tetrodotoxin (TTX), ω-agatoxin IVA, ω-conotoxin GVIA, SNX-482 (all from Alomone Labs, Jerusalem, Israel), (±)-norepinephrine, serotonin hydrochloride (5-hydroxytryptamine, 5-HT) and CdCl 2 (all from Sigma-Aldrich).

Techniques: Membrane, MANN-WHITNEY, Concentration Assay, Blocking Assay, Activation Assay