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Image Search Results
Journal: Regenerative therapy
Article Title: Xenogeneic-free defined conditions for derivation and expansion of human embryonic stem cells with mesenchymal stem cells.
doi: 10.1016/j.reth.2014.12.004
Figure Lengend Snippet: Fig. 4. Pluripotent marker expression of xenogeneic-free SEES cell lines In the undifferentiated state, xenogeneic-free SEES cell lines expressed markers characteristic of pluripotent hESCs, including OCT4, NANOG, SOX2, SSEA4, and TRA1-60. SEES-4: scale bars are 200 mm; SEES-5: scale bars are 50 mm in OCT3/4 and TRA1-60 and 200 mm in SOX2 and SSEA4; SEES-6: scale bars are 50 mm in OCT3/4 and SOX2 and 200 mm in SSEA4; SEES-7: scale bars are 200 mm.
Article Snippet: Antibodies against human SSEA-1 (R&D Systems; #FAB2155C),
Techniques: Marker, Expressing
Journal: Regenerative therapy
Article Title: Xenogeneic-free defined conditions for derivation and expansion of human embryonic stem cells with mesenchymal stem cells.
doi: 10.1016/j.reth.2014.12.004
Figure Lengend Snippet: Fig. 6. Characterization of the pluripotency of SEES-2 maintained using a modified conventional hESC culture medium SEES-2 cells were stably maintained over 20 passages on the qualified MEF feeder layer in the modified medium, which contained pharmaceutical-grade recombinant human bFGF (trafermin) and high-dose (35-K) gamma-irradiated KO-SR without antibiotics. A) Typical hESC colony morphology was readily visible. ALP activity was detected. B) SEES-2 cells expressed undifferentiated hESC markers, including OCT4, NANOG, SOX2, SSEA4, and TRA1-60. SEES-2 cells could differentiate into three embryonic germ layers in vitro and in vivo. Scale bars are 200 mm. C) SEES cells that had been differentiated in vitro via EBs expressed markers of the primary germ layers, ectoderm (TUJ1), mesoderm (aSMA), and endoderm (AFP). Scale bars are 100 mm. D) Histological analysis of teratomas containing multidifferentiated tissues derived from SEES-2 cells. Pigmented epithelium (ectoderm), cartilage (mesoderm), and gut epithelial tissues (endoderm). E) Chromosomal analysis of SEES-2 cells cultivated through 16 passages using a modified conventional hESC culture medium showed a normal 46,XX karyotype.
Article Snippet: Antibodies against human SSEA-1 (R&D Systems; #FAB2155C),
Techniques: Stable Transfection, Recombinant, Irradiation, Activity Assay, In Vitro, In Vivo, Derivative Assay
Journal: Regenerative therapy
Article Title: Xenogeneic-free defined conditions for derivation and expansion of human embryonic stem cells with mesenchymal stem cells.
doi: 10.1016/j.reth.2014.12.004
Figure Lengend Snippet: Fig. 7. Expression of pluripotency markers in SEES-2 cells was maintained using a modified conventional hESC culture medium Flow cytometric analysis of hESC-specific marker expression in SEES-2 cells. The isotype control is indicated by the blue line, and the unlabeled sample, which was used as a control, is indicated by the red line. Surface staining is shown by the yellow line for SSEA4, SSEA1, TRA-1-60, and TRA-1-81.
Article Snippet: Antibodies against human SSEA-1 (R&D Systems; #FAB2155C),
Techniques: Expressing, Marker, Control, Staining