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Image Search Results
Journal: Cell stem cell
Article Title: The microbiota regulates hematopoietic stem cell fate decisions by controlling iron availability in bone marrow
doi: 10.1016/j.stem.2021.12.009
Figure Lengend Snippet: (A–C) Cell lineages, Lin − cells, MPPs and HSCs in the BM of control and ABX-treated mice at day 24 after sublethal TBI (n = 4–5). See also Figure S2G . (D–E) BMT and HSCT analyses of control and ABX-treated mice under steady state or at day 24 after sublethal TBI (n = 4–9). (F) HSCs in the blood and BM of control and ABX-treated mice following G-CSF treatment (n = 6–8). (G–I) LSK cells and HSCs in the BM of control and ABX-treated mice following E. coli infection or LPS challenge (n = 3–4). See also Figure S2H – I . * p < 0.05, ** p < 0.01, *** p < 0.001. Error bars, mean ± SEM. See also Figure S3 for niche, HSC localization, and cytokine availability analyses.
Article Snippet:
Techniques: Control, Infection
Journal: Cell reports
Article Title: A combinatorial approach to uncover an additional Integrator subunit
doi: 10.1016/j.celrep.2023.112244
Figure Lengend Snippet: (A) FLAG IP of a stably expressing INTS15-FLAG HEK-293T cell line. Western blot was probed for INTS1, INTS5, INTS6, INTS8, INTS10, INTS13, INTS14, RNAPII (RPB1), and INTS15 (FLAG). Input lane is 5% of the starting material. (B) Silver stain of INTS15-FLAG IP eluate shown in (A). Labels indicate bands that were excised and identified through LC-MS/MS. (C) IP-MS of FLAG IPs from INTS15-FLAG and GFP-FLAG nuclear extracts. Correlation plot compares log 10 iBAQ values for each IP-MS. (D) Schematic of experimental design for (E). A FLAG IP was performed on the INTS15-FLAG stable HEK-293T cell line, followed by glycerol gradient. (E) Glycerol gradient fractionation of INTS15-FLAG IP. Fraction 1 represents 50% glycerol, while fraction 14 represents 11% glycerol. Integrator subunits INTS1, INTS13, INTS10, and INTS15 (FLAG) were probed in the western blot. (F) FLAG IP western blot of nuclear INTS15-FLAG performed in different salt conditions (100–750 mM KCl). Salt conditions indicated were used in both the IP buffer and wash buffers. Input is 2% of starting material. Western blot was probed for RNAPII (Rpb1), INTS1, INTS3, INTS5, INTS6, INTS10, INTS13, and INTS15-FLAG. (G) Endogenous co-IPs using rabbit sera from two house-made polyclonal antibodies raised against recombinant INTS15 with rabbit immunoglobulin G (IgG) used as a control. Input represents 5% of the original starting material. INTS1, INTS3, INTS13, and INTS15 were probed in the western blot. See .
Article Snippet: Anti-INTS3,
Techniques: Stable Transfection, Expressing, Western Blot, Silver Staining, Liquid Chromatography with Mass Spectroscopy, Protein-Protein interactions, Fractionation, Recombinant, Control
Journal: Cell reports
Article Title: A combinatorial approach to uncover an additional Integrator subunit
doi: 10.1016/j.celrep.2023.112244
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet: Anti-INTS3,
Techniques: Virus, Recombinant, Multiplex Assay, cDNA Synthesis, Western Blot, Mass Spectrometry, Sequencing, Software