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Image Search Results
Journal: EMBO Reports
Article Title: Ubiquitylation of BBSome is required for ciliary assembly and signaling
doi: 10.15252/embr.202255571
Figure Lengend Snippet: ARPE‐19 cells were transiently transfected with control siRNA or siRNA targeting PJA2 (si PJA2 ), serum‐deprived for 48 h, and immunostained for acetylated tubulin (red). Nuclei were stained with DAPI (blue). Scale bar, 3 μm. Lower panel shows western blot for PJA2. Cumulative data of the experiments shown in (A). A mean value ±SD of at least 4 independent experiments is shown. In total 25 cells for each experiment were scored. Student's t test, ** P < 0.01. ARPE‐19 cells were transiently transfected with control siRNA or siRNA targeting PJA2 (si PJA2 ). Cells were serum‐deprived for 48 h and left untreated or stimulated with purmorphamine (puro) for 4 h. Cells were fixed and doubly immunostained for GPR161 (green) and acetylated tubulin (red). Nuclei were stained with DAPI (blue). Scale bar, 3 μm. Cumulative data of the experiments shown in (C). A mean value ±SD of at least 4 independent experiments is shown. In total, 25 cells for each experiment were scored. Student's t test, ** P < 0.01. ARPE‐19 cells were transiently transfected with control siRNA or siRNA targeting PJA2 (si PJA2 ). Cells were serum‐deprived for 48 h and left untreated or stimulated with somatostatin (SST) for 3 h. Cells were fixed and doubly immunostained for SSTR3 (green) and acetylated tubulin (red). Nuclei were stained with DAPI (blue). Scale bar, 3 μm. Cumulative data of the experiments shown in (E). A mean value ±SD of at least 4 independent experiments is shown. In total 25 cells for each experiment were scored. Student's t test, *** P < 0.001.
Article Snippet: Primary antibodies against the following epitopes were used: flag (1:5,000 immunoblot, 1:200 immunoprecipitation; #F3165, Sigma‐Aldrich), GST (1:5,000; #sc‐138 Santa Cruz Biotechnology), PJA2 (1:1,000 immunoblot, 1:200 immunoprecipitation, 1:200 immunofluorescence; #A302‐991A, Bethyl Laboratories), GPR161 (1:100 immunofluorescence #13398‐1‐AP, Proteintech),
Techniques: Transfection, Control, Staining, Western Blot