srx Search Results


rabbit polyclonal anti sulfiredoxin  (Proteintech)
92
Proteintech rabbit polyclonal anti sulfiredoxin
Rabbit Polyclonal Anti Sulfiredoxin, supplied by Proteintech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/srx/SRX1+Antibody/pmc06269445-172-152-156
Average 92 stars, based on 1 article reviews
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human srxn 1  (OriGene)
92
OriGene human srxn 1
Human Srxn 1, supplied by OriGene, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/srx/SRXN1+(NM_080725)+Human+Tagged+ORF+Clone/pmc07502373-260-18-22
Average 92 stars, based on 1 article reviews
human srxn 1 - by Bioz Stars, 2026-10
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human srx cdna  (OriGene)
90
OriGene human srx cdna
Fig. 4. <t>Antioxidant</t> <t>Srx</t> is involved in AGP-resistance in HT29 colorectal cancer cells. (A&B) HT29 cancer cells were treated with AGP for the indicated time points. Srx gene expression was measured by RT-qPCR and Western blotting. (C) Western blot analysis of Srx protein expression in treated and un-treated control cells. HT29 and Caco2 cells were pre-treated with NAC for 1–2 h and then treated with AGP for 30 s. GAPDH antibody was used as loading control. The relative bar graphs for Srx protein. The band intensities were normalized against that of GAPDH. Data are expressed as the mean ± SD, n = 3. *p ≤.05 (D) HT29 cells were transfected with Srx siRNA or control siRNA for 24 h. Srx protein expression was measured by using specific antibody to Srx protein. GAPDH antibody was used as loading control. The relative bar graphs for Srx protein. The band intensities were normalized against that of GAPDH. Data are expressed as the mean ± SD, n = 3. *p ≤.05. (E) Caco2 cells were transfected with Srx <t>cDNA</t> plasmid (pSrx) or a control vector plasmid (pControl) for 24–36 h. Srx protein expression was measured by using specific antibody to Srx protein. GAPDH antibody was used as a loading control. The relative bar graphs for Srx protein. The band intensities were normalized against that of GAPDH. Data are expressed as the mean ± SD, n = 3. *p ≤.05. (F) HT29 and Caco2 cells were transfected with siControl or siSrx siRNAs for 24 h, followed with AGP treatment for the indicated time points. Cell viability was measured using MTS/PMS assay 24 h after treatment. (G) Caco2 cells were transfected with Srx cDNA plasmid (pSrx) or control vector plasmid (pControl) for 24 h, followed with AGP treatment for indicated time points. Cell viability was measured using MTS/PMS assay 24 h after AGP treatment. Control cells were treated only with He gas flow in all experiments. All values are mean ± of three independent experiments performed in triplicate. *p ≤.05; ANOVA.
Human Srx Cdna, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/srx/SRXN1+(NM_080725)+Human+Tagged+ORF+Clone/pm25173814-70-0-15
Average 90 stars, based on 1 article reviews
human srx cdna - by Bioz Stars, 2026-10
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srx software  (Vutara Inc)
90
Vutara Inc srx software
Fig. 4. <t>Antioxidant</t> <t>Srx</t> is involved in AGP-resistance in HT29 colorectal cancer cells. (A&B) HT29 cancer cells were treated with AGP for the indicated time points. Srx gene expression was measured by RT-qPCR and Western blotting. (C) Western blot analysis of Srx protein expression in treated and un-treated control cells. HT29 and Caco2 cells were pre-treated with NAC for 1–2 h and then treated with AGP for 30 s. GAPDH antibody was used as loading control. The relative bar graphs for Srx protein. The band intensities were normalized against that of GAPDH. Data are expressed as the mean ± SD, n = 3. *p ≤.05 (D) HT29 cells were transfected with Srx siRNA or control siRNA for 24 h. Srx protein expression was measured by using specific antibody to Srx protein. GAPDH antibody was used as loading control. The relative bar graphs for Srx protein. The band intensities were normalized against that of GAPDH. Data are expressed as the mean ± SD, n = 3. *p ≤.05. (E) Caco2 cells were transfected with Srx <t>cDNA</t> plasmid (pSrx) or a control vector plasmid (pControl) for 24–36 h. Srx protein expression was measured by using specific antibody to Srx protein. GAPDH antibody was used as a loading control. The relative bar graphs for Srx protein. The band intensities were normalized against that of GAPDH. Data are expressed as the mean ± SD, n = 3. *p ≤.05. (F) HT29 and Caco2 cells were transfected with siControl or siSrx siRNAs for 24 h, followed with AGP treatment for the indicated time points. Cell viability was measured using MTS/PMS assay 24 h after treatment. (G) Caco2 cells were transfected with Srx cDNA plasmid (pSrx) or control vector plasmid (pControl) for 24 h, followed with AGP treatment for indicated time points. Cell viability was measured using MTS/PMS assay 24 h after AGP treatment. Control cells were treated only with He gas flow in all experiments. All values are mean ± of three independent experiments performed in triplicate. *p ≤.05; ANOVA.
Srx Software, supplied by Vutara Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/srx/srx+software/pm40263458-335-0-0
Average 90 stars, based on 1 article reviews
srx software - by Bioz Stars, 2026-10
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vutara srx software  (Vutara Inc)
90
Vutara Inc vutara srx software
Fig. 4. <t>Antioxidant</t> <t>Srx</t> is involved in AGP-resistance in HT29 colorectal cancer cells. (A&B) HT29 cancer cells were treated with AGP for the indicated time points. Srx gene expression was measured by RT-qPCR and Western blotting. (C) Western blot analysis of Srx protein expression in treated and un-treated control cells. HT29 and Caco2 cells were pre-treated with NAC for 1–2 h and then treated with AGP for 30 s. GAPDH antibody was used as loading control. The relative bar graphs for Srx protein. The band intensities were normalized against that of GAPDH. Data are expressed as the mean ± SD, n = 3. *p ≤.05 (D) HT29 cells were transfected with Srx siRNA or control siRNA for 24 h. Srx protein expression was measured by using specific antibody to Srx protein. GAPDH antibody was used as loading control. The relative bar graphs for Srx protein. The band intensities were normalized against that of GAPDH. Data are expressed as the mean ± SD, n = 3. *p ≤.05. (E) Caco2 cells were transfected with Srx <t>cDNA</t> plasmid (pSrx) or a control vector plasmid (pControl) for 24–36 h. Srx protein expression was measured by using specific antibody to Srx protein. GAPDH antibody was used as a loading control. The relative bar graphs for Srx protein. The band intensities were normalized against that of GAPDH. Data are expressed as the mean ± SD, n = 3. *p ≤.05. (F) HT29 and Caco2 cells were transfected with siControl or siSrx siRNAs for 24 h, followed with AGP treatment for the indicated time points. Cell viability was measured using MTS/PMS assay 24 h after treatment. (G) Caco2 cells were transfected with Srx cDNA plasmid (pSrx) or control vector plasmid (pControl) for 24 h, followed with AGP treatment for indicated time points. Cell viability was measured using MTS/PMS assay 24 h after AGP treatment. Control cells were treated only with He gas flow in all experiments. All values are mean ± of three independent experiments performed in triplicate. *p ≤.05; ANOVA.
Vutara Srx Software, supplied by Vutara Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/srx/vutara+srx+software/10__1096_slash_fj__202000667r-85-14-13
Average 90 stars, based on 1 article reviews
vutara srx software - by Bioz Stars, 2026-10
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srx localization and visualization software v6.2  (Vutara Inc)
90
Vutara Inc srx localization and visualization software v6.2
Fig. 4. <t>Antioxidant</t> <t>Srx</t> is involved in AGP-resistance in HT29 colorectal cancer cells. (A&B) HT29 cancer cells were treated with AGP for the indicated time points. Srx gene expression was measured by RT-qPCR and Western blotting. (C) Western blot analysis of Srx protein expression in treated and un-treated control cells. HT29 and Caco2 cells were pre-treated with NAC for 1–2 h and then treated with AGP for 30 s. GAPDH antibody was used as loading control. The relative bar graphs for Srx protein. The band intensities were normalized against that of GAPDH. Data are expressed as the mean ± SD, n = 3. *p ≤.05 (D) HT29 cells were transfected with Srx siRNA or control siRNA for 24 h. Srx protein expression was measured by using specific antibody to Srx protein. GAPDH antibody was used as loading control. The relative bar graphs for Srx protein. The band intensities were normalized against that of GAPDH. Data are expressed as the mean ± SD, n = 3. *p ≤.05. (E) Caco2 cells were transfected with Srx <t>cDNA</t> plasmid (pSrx) or a control vector plasmid (pControl) for 24–36 h. Srx protein expression was measured by using specific antibody to Srx protein. GAPDH antibody was used as a loading control. The relative bar graphs for Srx protein. The band intensities were normalized against that of GAPDH. Data are expressed as the mean ± SD, n = 3. *p ≤.05. (F) HT29 and Caco2 cells were transfected with siControl or siSrx siRNAs for 24 h, followed with AGP treatment for the indicated time points. Cell viability was measured using MTS/PMS assay 24 h after treatment. (G) Caco2 cells were transfected with Srx cDNA plasmid (pSrx) or control vector plasmid (pControl) for 24 h, followed with AGP treatment for indicated time points. Cell viability was measured using MTS/PMS assay 24 h after AGP treatment. Control cells were treated only with He gas flow in all experiments. All values are mean ± of three independent experiments performed in triplicate. *p ≤.05; ANOVA.
Srx Localization And Visualization Software V6.2, supplied by Vutara Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/srx/srx+v+6+04+14+analysis+software/pmc07399380-80-6-6
Average 90 stars, based on 1 article reviews
srx localization and visualization software v6.2 - by Bioz Stars, 2026-10
90/100 stars
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smooth round silicone implants inspira srx 420 cc  (InSpira LLC)
90
InSpira LLC smooth round silicone implants inspira srx 420 cc
Fig. 4. <t>Antioxidant</t> <t>Srx</t> is involved in AGP-resistance in HT29 colorectal cancer cells. (A&B) HT29 cancer cells were treated with AGP for the indicated time points. Srx gene expression was measured by RT-qPCR and Western blotting. (C) Western blot analysis of Srx protein expression in treated and un-treated control cells. HT29 and Caco2 cells were pre-treated with NAC for 1–2 h and then treated with AGP for 30 s. GAPDH antibody was used as loading control. The relative bar graphs for Srx protein. The band intensities were normalized against that of GAPDH. Data are expressed as the mean ± SD, n = 3. *p ≤.05 (D) HT29 cells were transfected with Srx siRNA or control siRNA for 24 h. Srx protein expression was measured by using specific antibody to Srx protein. GAPDH antibody was used as loading control. The relative bar graphs for Srx protein. The band intensities were normalized against that of GAPDH. Data are expressed as the mean ± SD, n = 3. *p ≤.05. (E) Caco2 cells were transfected with Srx <t>cDNA</t> plasmid (pSrx) or a control vector plasmid (pControl) for 24–36 h. Srx protein expression was measured by using specific antibody to Srx protein. GAPDH antibody was used as a loading control. The relative bar graphs for Srx protein. The band intensities were normalized against that of GAPDH. Data are expressed as the mean ± SD, n = 3. *p ≤.05. (F) HT29 and Caco2 cells were transfected with siControl or siSrx siRNAs for 24 h, followed with AGP treatment for the indicated time points. Cell viability was measured using MTS/PMS assay 24 h after treatment. (G) Caco2 cells were transfected with Srx cDNA plasmid (pSrx) or control vector plasmid (pControl) for 24 h, followed with AGP treatment for indicated time points. Cell viability was measured using MTS/PMS assay 24 h after AGP treatment. Control cells were treated only with He gas flow in all experiments. All values are mean ± of three independent experiments performed in triplicate. *p ≤.05; ANOVA.
Smooth Round Silicone Implants Inspira Srx 420 Cc, supplied by InSpira LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/srx/smooth+round+silicone+implants+inspira+srx+420+cc/pmc07317085-104-91-94
Average 90 stars, based on 1 article reviews
smooth round silicone implants inspira srx 420 cc - by Bioz Stars, 2026-10
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rabbit antiserum specific for purified s. cerevisiae srx  (Centre Lago)
90
Centre Lago rabbit antiserum specific for purified s. cerevisiae srx
Fig. 4. <t>Antioxidant</t> <t>Srx</t> is involved in AGP-resistance in HT29 colorectal cancer cells. (A&B) HT29 cancer cells were treated with AGP for the indicated time points. Srx gene expression was measured by RT-qPCR and Western blotting. (C) Western blot analysis of Srx protein expression in treated and un-treated control cells. HT29 and Caco2 cells were pre-treated with NAC for 1–2 h and then treated with AGP for 30 s. GAPDH antibody was used as loading control. The relative bar graphs for Srx protein. The band intensities were normalized against that of GAPDH. Data are expressed as the mean ± SD, n = 3. *p ≤.05 (D) HT29 cells were transfected with Srx siRNA or control siRNA for 24 h. Srx protein expression was measured by using specific antibody to Srx protein. GAPDH antibody was used as loading control. The relative bar graphs for Srx protein. The band intensities were normalized against that of GAPDH. Data are expressed as the mean ± SD, n = 3. *p ≤.05. (E) Caco2 cells were transfected with Srx <t>cDNA</t> plasmid (pSrx) or a control vector plasmid (pControl) for 24–36 h. Srx protein expression was measured by using specific antibody to Srx protein. GAPDH antibody was used as a loading control. The relative bar graphs for Srx protein. The band intensities were normalized against that of GAPDH. Data are expressed as the mean ± SD, n = 3. *p ≤.05. (F) HT29 and Caco2 cells were transfected with siControl or siSrx siRNAs for 24 h, followed with AGP treatment for the indicated time points. Cell viability was measured using MTS/PMS assay 24 h after treatment. (G) Caco2 cells were transfected with Srx cDNA plasmid (pSrx) or control vector plasmid (pControl) for 24 h, followed with AGP treatment for indicated time points. Cell viability was measured using MTS/PMS assay 24 h after AGP treatment. Control cells were treated only with He gas flow in all experiments. All values are mean ± of three independent experiments performed in triplicate. *p ≤.05; ANOVA.
Rabbit Antiserum Specific For Purified S. Cerevisiae Srx, supplied by Centre Lago, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/srx/rabbit+antiserum+specific+for+purified+s++cerevisiae+srx/10__1074_slash_jbc__m800493200-55-0-11
Average 90 stars, based on 1 article reviews
rabbit antiserum specific for purified s. cerevisiae srx - by Bioz Stars, 2026-10
90/100 stars
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srx storm system  (Vutara Inc)
90
Vutara Inc srx storm system
Fig. 4. <t>Antioxidant</t> <t>Srx</t> is involved in AGP-resistance in HT29 colorectal cancer cells. (A&B) HT29 cancer cells were treated with AGP for the indicated time points. Srx gene expression was measured by RT-qPCR and Western blotting. (C) Western blot analysis of Srx protein expression in treated and un-treated control cells. HT29 and Caco2 cells were pre-treated with NAC for 1–2 h and then treated with AGP for 30 s. GAPDH antibody was used as loading control. The relative bar graphs for Srx protein. The band intensities were normalized against that of GAPDH. Data are expressed as the mean ± SD, n = 3. *p ≤.05 (D) HT29 cells were transfected with Srx siRNA or control siRNA for 24 h. Srx protein expression was measured by using specific antibody to Srx protein. GAPDH antibody was used as loading control. The relative bar graphs for Srx protein. The band intensities were normalized against that of GAPDH. Data are expressed as the mean ± SD, n = 3. *p ≤.05. (E) Caco2 cells were transfected with Srx <t>cDNA</t> plasmid (pSrx) or a control vector plasmid (pControl) for 24–36 h. Srx protein expression was measured by using specific antibody to Srx protein. GAPDH antibody was used as a loading control. The relative bar graphs for Srx protein. The band intensities were normalized against that of GAPDH. Data are expressed as the mean ± SD, n = 3. *p ≤.05. (F) HT29 and Caco2 cells were transfected with siControl or siSrx siRNAs for 24 h, followed with AGP treatment for the indicated time points. Cell viability was measured using MTS/PMS assay 24 h after treatment. (G) Caco2 cells were transfected with Srx cDNA plasmid (pSrx) or control vector plasmid (pControl) for 24 h, followed with AGP treatment for indicated time points. Cell viability was measured using MTS/PMS assay 24 h after AGP treatment. Control cells were treated only with He gas flow in all experiments. All values are mean ± of three independent experiments performed in triplicate. *p ≤.05; ANOVA.
Srx Storm System, supplied by Vutara Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/srx/srx+storm+system/pmc06795522-253-10-9
Average 90 stars, based on 1 article reviews
srx storm system - by Bioz Stars, 2026-10
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srx-r105  (Sony)
90
Sony srx-r105
Fig. 4. <t>Antioxidant</t> <t>Srx</t> is involved in AGP-resistance in HT29 colorectal cancer cells. (A&B) HT29 cancer cells were treated with AGP for the indicated time points. Srx gene expression was measured by RT-qPCR and Western blotting. (C) Western blot analysis of Srx protein expression in treated and un-treated control cells. HT29 and Caco2 cells were pre-treated with NAC for 1–2 h and then treated with AGP for 30 s. GAPDH antibody was used as loading control. The relative bar graphs for Srx protein. The band intensities were normalized against that of GAPDH. Data are expressed as the mean ± SD, n = 3. *p ≤.05 (D) HT29 cells were transfected with Srx siRNA or control siRNA for 24 h. Srx protein expression was measured by using specific antibody to Srx protein. GAPDH antibody was used as loading control. The relative bar graphs for Srx protein. The band intensities were normalized against that of GAPDH. Data are expressed as the mean ± SD, n = 3. *p ≤.05. (E) Caco2 cells were transfected with Srx <t>cDNA</t> plasmid (pSrx) or a control vector plasmid (pControl) for 24–36 h. Srx protein expression was measured by using specific antibody to Srx protein. GAPDH antibody was used as a loading control. The relative bar graphs for Srx protein. The band intensities were normalized against that of GAPDH. Data are expressed as the mean ± SD, n = 3. *p ≤.05. (F) HT29 and Caco2 cells were transfected with siControl or siSrx siRNAs for 24 h, followed with AGP treatment for the indicated time points. Cell viability was measured using MTS/PMS assay 24 h after treatment. (G) Caco2 cells were transfected with Srx cDNA plasmid (pSrx) or control vector plasmid (pControl) for 24 h, followed with AGP treatment for indicated time points. Cell viability was measured using MTS/PMS assay 24 h after AGP treatment. Control cells were treated only with He gas flow in all experiments. All values are mean ± of three independent experiments performed in triplicate. *p ≤.05; ANOVA.
Srx R105, supplied by Sony, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/srx/srx+r105/us07996699-170-28-19
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srx-r105 - by Bioz Stars, 2026-10
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oligonucleotides containing the small interfering rna sequences targeting srx  (Genotec GmbH)
90
Genotec GmbH oligonucleotides containing the small interfering rna sequences targeting srx
Fig. 4. <t>Antioxidant</t> <t>Srx</t> is involved in AGP-resistance in HT29 colorectal cancer cells. (A&B) HT29 cancer cells were treated with AGP for the indicated time points. Srx gene expression was measured by RT-qPCR and Western blotting. (C) Western blot analysis of Srx protein expression in treated and un-treated control cells. HT29 and Caco2 cells were pre-treated with NAC for 1–2 h and then treated with AGP for 30 s. GAPDH antibody was used as loading control. The relative bar graphs for Srx protein. The band intensities were normalized against that of GAPDH. Data are expressed as the mean ± SD, n = 3. *p ≤.05 (D) HT29 cells were transfected with Srx siRNA or control siRNA for 24 h. Srx protein expression was measured by using specific antibody to Srx protein. GAPDH antibody was used as loading control. The relative bar graphs for Srx protein. The band intensities were normalized against that of GAPDH. Data are expressed as the mean ± SD, n = 3. *p ≤.05. (E) Caco2 cells were transfected with Srx <t>cDNA</t> plasmid (pSrx) or a control vector plasmid (pControl) for 24–36 h. Srx protein expression was measured by using specific antibody to Srx protein. GAPDH antibody was used as a loading control. The relative bar graphs for Srx protein. The band intensities were normalized against that of GAPDH. Data are expressed as the mean ± SD, n = 3. *p ≤.05. (F) HT29 and Caco2 cells were transfected with siControl or siSrx siRNAs for 24 h, followed with AGP treatment for the indicated time points. Cell viability was measured using MTS/PMS assay 24 h after treatment. (G) Caco2 cells were transfected with Srx cDNA plasmid (pSrx) or control vector plasmid (pControl) for 24 h, followed with AGP treatment for indicated time points. Cell viability was measured using MTS/PMS assay 24 h after AGP treatment. Control cells were treated only with He gas flow in all experiments. All values are mean ± of three independent experiments performed in triplicate. *p ≤.05; ANOVA.
Oligonucleotides Containing The Small Interfering Rna Sequences Targeting Srx, supplied by Genotec GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/srx/oligonucleotides+containing+the+small+interfering+rna+sequences+targeting+srx/pmc03249139-54-23-41
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Image Search Results


Fig. 4. Antioxidant Srx is involved in AGP-resistance in HT29 colorectal cancer cells. (A&B) HT29 cancer cells were treated with AGP for the indicated time points. Srx gene expression was measured by RT-qPCR and Western blotting. (C) Western blot analysis of Srx protein expression in treated and un-treated control cells. HT29 and Caco2 cells were pre-treated with NAC for 1–2 h and then treated with AGP for 30 s. GAPDH antibody was used as loading control. The relative bar graphs for Srx protein. The band intensities were normalized against that of GAPDH. Data are expressed as the mean ± SD, n = 3. *p ≤.05 (D) HT29 cells were transfected with Srx siRNA or control siRNA for 24 h. Srx protein expression was measured by using specific antibody to Srx protein. GAPDH antibody was used as loading control. The relative bar graphs for Srx protein. The band intensities were normalized against that of GAPDH. Data are expressed as the mean ± SD, n = 3. *p ≤.05. (E) Caco2 cells were transfected with Srx cDNA plasmid (pSrx) or a control vector plasmid (pControl) for 24–36 h. Srx protein expression was measured by using specific antibody to Srx protein. GAPDH antibody was used as a loading control. The relative bar graphs for Srx protein. The band intensities were normalized against that of GAPDH. Data are expressed as the mean ± SD, n = 3. *p ≤.05. (F) HT29 and Caco2 cells were transfected with siControl or siSrx siRNAs for 24 h, followed with AGP treatment for the indicated time points. Cell viability was measured using MTS/PMS assay 24 h after treatment. (G) Caco2 cells were transfected with Srx cDNA plasmid (pSrx) or control vector plasmid (pControl) for 24 h, followed with AGP treatment for indicated time points. Cell viability was measured using MTS/PMS assay 24 h after AGP treatment. Control cells were treated only with He gas flow in all experiments. All values are mean ± of three independent experiments performed in triplicate. *p ≤.05; ANOVA.

Journal: Biochimica et biophysica acta

Article Title: Atmospheric pressure gas plasma-induced colorectal cancer cell death is mediated by Nox2-ASK1 apoptosis pathways and oxidative stress is mitigated by Srx-Nrf2 anti-oxidant system.

doi: 10.1016/j.bbamcr.2014.08.011

Figure Lengend Snippet: Fig. 4. Antioxidant Srx is involved in AGP-resistance in HT29 colorectal cancer cells. (A&B) HT29 cancer cells were treated with AGP for the indicated time points. Srx gene expression was measured by RT-qPCR and Western blotting. (C) Western blot analysis of Srx protein expression in treated and un-treated control cells. HT29 and Caco2 cells were pre-treated with NAC for 1–2 h and then treated with AGP for 30 s. GAPDH antibody was used as loading control. The relative bar graphs for Srx protein. The band intensities were normalized against that of GAPDH. Data are expressed as the mean ± SD, n = 3. *p ≤.05 (D) HT29 cells were transfected with Srx siRNA or control siRNA for 24 h. Srx protein expression was measured by using specific antibody to Srx protein. GAPDH antibody was used as loading control. The relative bar graphs for Srx protein. The band intensities were normalized against that of GAPDH. Data are expressed as the mean ± SD, n = 3. *p ≤.05. (E) Caco2 cells were transfected with Srx cDNA plasmid (pSrx) or a control vector plasmid (pControl) for 24–36 h. Srx protein expression was measured by using specific antibody to Srx protein. GAPDH antibody was used as a loading control. The relative bar graphs for Srx protein. The band intensities were normalized against that of GAPDH. Data are expressed as the mean ± SD, n = 3. *p ≤.05. (F) HT29 and Caco2 cells were transfected with siControl or siSrx siRNAs for 24 h, followed with AGP treatment for the indicated time points. Cell viability was measured using MTS/PMS assay 24 h after treatment. (G) Caco2 cells were transfected with Srx cDNA plasmid (pSrx) or control vector plasmid (pControl) for 24 h, followed with AGP treatment for indicated time points. Cell viability was measured using MTS/PMS assay 24 h after AGP treatment. Control cells were treated only with He gas flow in all experiments. All values are mean ± of three independent experiments performed in triplicate. *p ≤.05; ANOVA.

Article Snippet: Human Srx cDNA clone plasmid (Cat#RG207654) and control vector pCMV6-AC-GFP (Cat# PS100010) were purchased from OriGene Technologies.

Techniques: Gene Expression, Quantitative RT-PCR, Western Blot, Expressing, Control, Transfection, Plasmid Preparation