Journal: Frontiers in Microbiology

Article Title: Detection of Cronobacter Genus in Powdered Infant Formula by Enzyme-linked Immunosorbent Assay Using Anti- Cronobacter Antibody

doi: 10.3389/fmicb.2016.01124

Figure Lengend Snippet: Cross-reactivity of the developed indirect non-competitive enzyme-linked immunosorbent assay with non- Cronobacter species in pure culture.

Article Snippet: B. cereus (KCCM 40935), Buttiauxella noackiae (ATCC 51713), C. condimenti (LMG 26250), C. dublinensis (LMG 23823), C. malonaticus (LMG 23826), C. muytjensii (CDC 3523-75), C. turicensis (LMG 23827), C. muytjensii (ATCC 51329), C. sakazakii (ATCC 29544), C. sakazakii (ATCC 29004), C. universalis (LMG 26249), C. freundi (ATCC 8090), Escherichia coli (ATCC 39418), Franconibacter helveticus (LMG 23732), Franconibacter pulveris (LMG 24057), and Salmonella Typhimurium (ATCC 13311) were used in this study.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Saudi Journal of Biological Sciences

Article Title: A comparative ex vivo permeation evaluation of a novel 5-Fluorocuracil nanoemulsion-gel by topically applied in the different excised rat, goat, and cow skin

doi: 10.1016/j.sjbs.2020.02.014

Figure Lengend Snippet: Solubility of 5-FU determined in different types of oils and surfactants.

Article Snippet: 5-Fluorouracil was obtained from Chem-Impex International, Inc. Milli-Q-water purification system (ELGA, Made in UK) was used for purification of water i.e. Milli-Q-water.

Techniques: Solubility

Journal: Saudi Journal of Biological Sciences

Article Title: A comparative ex vivo permeation evaluation of a novel 5-Fluorocuracil nanoemulsion-gel by topically applied in the different excised rat, goat, and cow skin

doi: 10.1016/j.sjbs.2020.02.014

Figure Lengend Snippet: 5-FU-DDTC complex formation by Keto-enol mechanism.

Article Snippet: 5-Fluorouracil was obtained from Chem-Impex International, Inc. Milli-Q-water purification system (ELGA, Made in UK) was used for purification of water i.e. Milli-Q-water.

Techniques:

Journal: Saudi Journal of Biological Sciences

Article Title: A comparative ex vivo permeation evaluation of a novel 5-Fluorocuracil nanoemulsion-gel by topically applied in the different excised rat, goat, and cow skin

doi: 10.1016/j.sjbs.2020.02.014

Figure Lengend Snippet: Distribution of 5-FU-NE is determined by dynamic light scattering techniques (A), zeta potential of 5-FU-NE (B), SEM images (C), and TEM images (D).

Article Snippet: 5-Fluorouracil was obtained from Chem-Impex International, Inc. Milli-Q-water purification system (ELGA, Made in UK) was used for purification of water i.e. Milli-Q-water.

Techniques: Zeta Potential Analyzer

Journal: Saudi Journal of Biological Sciences

Article Title: A comparative ex vivo permeation evaluation of a novel 5-Fluorocuracil nanoemulsion-gel by topically applied in the different excised rat, goat, and cow skin

doi: 10.1016/j.sjbs.2020.02.014

Figure Lengend Snippet: DSC-thermograms showed endothermic peaks of castor oil, 5-FU, Transcutol HP, PEG-400, 5-FU-NE, Carbopol and freeze dried optimized-5-FU-NE-Gel.

Article Snippet: 5-Fluorouracil was obtained from Chem-Impex International, Inc. Milli-Q-water purification system (ELGA, Made in UK) was used for purification of water i.e. Milli-Q-water.

Techniques:

Journal: Saudi Journal of Biological Sciences

Article Title: A comparative ex vivo permeation evaluation of a novel 5-Fluorocuracil nanoemulsion-gel by topically applied in the different excised rat, goat, and cow skin

doi: 10.1016/j.sjbs.2020.02.014

Figure Lengend Snippet: Spectral analysis of ATR of castor oil, 5-FU, Transcutol HP, PEG-400, 5-FU-NE, Carbopol and freeze dried optimized-5-FU-NE-Gel.

Article Snippet: 5-Fluorouracil was obtained from Chem-Impex International, Inc. Milli-Q-water purification system (ELGA, Made in UK) was used for purification of water i.e. Milli-Q-water.

Techniques:

Journal: Saudi Journal of Biological Sciences

Article Title: A comparative ex vivo permeation evaluation of a novel 5-Fluorocuracil nanoemulsion-gel by topically applied in the different excised rat, goat, and cow skin

doi: 10.1016/j.sjbs.2020.02.014

Figure Lengend Snippet: showed the %age-cumulative-release of 5-FU from 5-FU-NE and 5-FU-NE-Gel when it compared with pure 5-FU-S.

Article Snippet: 5-Fluorouracil was obtained from Chem-Impex International, Inc. Milli-Q-water purification system (ELGA, Made in UK) was used for purification of water i.e. Milli-Q-water.

Techniques:

Journal: Saudi Journal of Biological Sciences

Article Title: A comparative ex vivo permeation evaluation of a novel 5-Fluorocuracil nanoemulsion-gel by topically applied in the different excised rat, goat, and cow skin

doi: 10.1016/j.sjbs.2020.02.014

Figure Lengend Snippet: showed a comparative ex vivo various skins (cow, goat, & rat) permeation study of 5-FU from 5-FU-NE and 5-FU-NE-Gel.

Article Snippet: 5-Fluorouracil was obtained from Chem-Impex International, Inc. Milli-Q-water purification system (ELGA, Made in UK) was used for purification of water i.e. Milli-Q-water.

Techniques: Ex Vivo

Journal: Saudi Journal of Biological Sciences

Article Title: A comparative ex vivo permeation evaluation of a novel 5-Fluorocuracil nanoemulsion-gel by topically applied in the different excised rat, goat, and cow skin

doi: 10.1016/j.sjbs.2020.02.014

Figure Lengend Snippet: %Age permeation of 5-FU and their retention of skin in stratum corneum (SC) and viable part of the skin ex-vivo from 5-FU, 5-FU-NE, and 5-FU-NE-Gel nanoformulation.

Article Snippet: 5-Fluorouracil was obtained from Chem-Impex International, Inc. Milli-Q-water purification system (ELGA, Made in UK) was used for purification of water i.e. Milli-Q-water.

Techniques: Ex Vivo

Journal: Saudi Journal of Biological Sciences

Article Title: A comparative ex vivo permeation evaluation of a novel 5-Fluorocuracil nanoemulsion-gel by topically applied in the different excised rat, goat, and cow skin

doi: 10.1016/j.sjbs.2020.02.014

Figure Lengend Snippet: Mass spectrum of 5-FU-MS-Scan i.e. protonated precursor [M-H] – ions at m / z 129 [A], 5-FU-MSMS-Scan fragmentation transitions i.e. major fragmented product ion at m / z 42 [B], and a chromatogram of UHPLC showing retention time 1.710 min.

Article Snippet: 5-Fluorouracil was obtained from Chem-Impex International, Inc. Milli-Q-water purification system (ELGA, Made in UK) was used for purification of water i.e. Milli-Q-water.

Techniques:

Journal: Saudi Journal of Biological Sciences

Article Title: A comparative ex vivo permeation evaluation of a novel 5-Fluorocuracil nanoemulsion-gel by topically applied in the different excised rat, goat, and cow skin

doi: 10.1016/j.sjbs.2020.02.014

Figure Lengend Snippet: Mass spectrum of Diethyldithiocarbamate (DDTC)-MS-Scan and Sodium-Diethyldithiocarbamate (DDTC)-MS-Scan showed at protonated precursor [M-H] – ions at m / z 147.27, and 170.21 [A], MS-Scan of complex 5-FU with Diethyldithiocarbamate (DDTC)-MS-Scan and Sodium-Diethyldithiocarbamate (DDTC)-MS-Scan product ion at m / z 277.09 and 300.26 [B], and a UHPLC chromatogram of showing retention time 0.736 and 2.185 min for DDTC chelating agent individual and DDTC–5-FU complex [C].

Article Snippet: 5-Fluorouracil was obtained from Chem-Impex International, Inc. Milli-Q-water purification system (ELGA, Made in UK) was used for purification of water i.e. Milli-Q-water.

Techniques:

Journal: Saudi Journal of Biological Sciences

Article Title: A comparative ex vivo permeation evaluation of a novel 5-Fluorocuracil nanoemulsion-gel by topically applied in the different excised rat, goat, and cow skin

doi: 10.1016/j.sjbs.2020.02.014

Figure Lengend Snippet: Fluorescence micrographs of cow- and goat-ear pinna skin and rat skin treated with the formulation 5-FU-S (A) 5-FU-NE (B) 5-FU-NE-Gel (C). Different type of formulations labelled with hydrophilic fluorescence probe (6-carboxyfluorescein) to mark the penetration of the 5-FU to deep skin strata.

Article Snippet: 5-Fluorouracil was obtained from Chem-Impex International, Inc. Milli-Q-water purification system (ELGA, Made in UK) was used for purification of water i.e. Milli-Q-water.

Techniques: Fluorescence, Formulation

Journal: Saudi Journal of Biological Sciences

Article Title: A comparative ex vivo permeation evaluation of a novel 5-Fluorocuracil nanoemulsion-gel by topically applied in the different excised rat, goat, and cow skin

doi: 10.1016/j.sjbs.2020.02.014

Figure Lengend Snippet: %Age-Cell viability (n = 3, SD, mean) of 5-FU in free 5-FU aqueous solution, control, and optimized-5-FU-NE, and 5-FU-NE-Gel nanoformulation on melanoma cell lines. Optimized–5-FU-NE3 (p < 0.05) and 5-FU-NE3-Gel (p < 0.01) were highly significant than free 5-FU-S.

Article Snippet: 5-Fluorouracil was obtained from Chem-Impex International, Inc. Milli-Q-water purification system (ELGA, Made in UK) was used for purification of water i.e. Milli-Q-water.

Techniques:

Journal: Saudi Journal of Biological Sciences

Article Title: A comparative ex vivo permeation evaluation of a novel 5-Fluorocuracil nanoemulsion-gel by topically applied in the different excised rat, goat, and cow skin

doi: 10.1016/j.sjbs.2020.02.014

Figure Lengend Snippet: IC50 and % Cytotoxicity of free 5-FU, opt-5-FU-NE, 5-FU-NE-Gel, and control on melanoma cancer cell lines (n = 3).

Article Snippet: 5-Fluorouracil was obtained from Chem-Impex International, Inc. Milli-Q-water purification system (ELGA, Made in UK) was used for purification of water i.e. Milli-Q-water.

Techniques: Concentration Assay

Journal: International Journal of Molecular Sciences

Article Title: Sorcin Inhibits Mitochondrial Apoptosis by Interacting with STAT3 via NF-κB Pathway

doi: 10.3390/ijms25137206

Figure Lengend Snippet: SRI and STAT3 are upregulated both in HCC tissues and cells. ( A , B ) SRI and STAT3 are highly expressed in HCC tissues (T) compared with normal liver tissues (N) in the GEO database (GDS4882). ( C ) Image available from Proteinatlas database showed high expression of SRI and STAT3 in tumor (T) compared with normal liver tissues (N). The expression of proteins were determined by the brown area. Scale bars = 200 μm. ( D – H ) Western blot showed SRI and STAT3 proteins were overexpressed in clinical HCC tissues and in Huh-7/HepG2/Hep3B cells. The red and blue spots represented the expression of different proteins in the T and N groups, respectively. ( I ) From the TGGA database, SRI was found to be positively correlated with STAT3 in LIHC ( p < 0.001). ( J , K ) The expressions of SRI and STAT3 are positively correlated in HCC tissues and cells lines ( p < 0.05). * p < 0.05, ** p < 0.01.

Article Snippet: The diluted primary antibodies against SRI (1:500, Proteintech, Wuhan, China), STAT3 (1:500, Proteintech, Wuhan, China), Bcl-XL (1:500, Proteintech, Wuhan, China), MCL-1 (1:500, Proteintech, Wuhan, China), p65 (1:500, Proteintech, Wuhan, China), p-p65 (1:500, Abmart, Shanghai, China), Bcl-2 (1:1000, Abcam, Cambridge, UK), Bax (1:1000, Proteintech, Wuhan, China), caspase3 (1:500, Proteintech, Wuhan, China), cIAP1 (1:500, Proteintech, Wuhan, China), p-IκB (1:500, Proteintech, Wuhan, China), tubulin (1:5000, Proteintech, Wuhan, China) and GAPDH (1:10000, Proteintech, Wuhan, China) were incubated with the membranes at 4 °C overnight.

Techniques: Expressing, Western Blot

Journal: International Journal of Molecular Sciences

Article Title: Sorcin Inhibits Mitochondrial Apoptosis by Interacting with STAT3 via NF-κB Pathway

doi: 10.3390/ijms25137206

Figure Lengend Snippet: SRI and STAT3 are interacting proteins. ( A ) SRI and STAT3 are interacting proteins in the STRING database. ( B ) SRI and STAT3 are interacting proteins confirmed by co-immunoprecipitation assays in Huh-7/HepG2 cells. ( C , D ) SRI (shown in red) co-localized with STAT3 (shown in green) was visualized by cellular immunofluorescence in Huh-7/HepG2 cells. DAPI (shown in blue) was used for nuclear staining. Scale bars = 200 μm.

Article Snippet: The diluted primary antibodies against SRI (1:500, Proteintech, Wuhan, China), STAT3 (1:500, Proteintech, Wuhan, China), Bcl-XL (1:500, Proteintech, Wuhan, China), MCL-1 (1:500, Proteintech, Wuhan, China), p65 (1:500, Proteintech, Wuhan, China), p-p65 (1:500, Abmart, Shanghai, China), Bcl-2 (1:1000, Abcam, Cambridge, UK), Bax (1:1000, Proteintech, Wuhan, China), caspase3 (1:500, Proteintech, Wuhan, China), cIAP1 (1:500, Proteintech, Wuhan, China), p-IκB (1:500, Proteintech, Wuhan, China), tubulin (1:5000, Proteintech, Wuhan, China) and GAPDH (1:10000, Proteintech, Wuhan, China) were incubated with the membranes at 4 °C overnight.

Techniques: Immunoprecipitation, Immunofluorescence, Staining

Journal: International Journal of Molecular Sciences

Article Title: Sorcin Inhibits Mitochondrial Apoptosis by Interacting with STAT3 via NF-κB Pathway

doi: 10.3390/ijms25137206

Figure Lengend Snippet: SRI interacts with STAT3, inhibits apoptosis and activates the NF-κB signaling pathway in vitro and in vivo. ( A – D ) SRI downexpression reduced the fluorescence intensity of TMRE (shown in red) and enhanced the apoptosis sensitivities by Hoechst 33342 (shown in blue) staining assay. The results of SRI overexpression were consisted with SRI downexpression. Scale bars = 200 μm. ( E – J ) Western blot detected the expression of STAT3, p65, p-p65, p-IκB and apoptosis-related proteins when SRI overexpression or downexpression were found in HCC cell and tumor xenografts. ( K , L ) Representative IHC images of STAT3, p65, p-p65, p-IκB and apoptosis-related proteins when SRI overexpression or downexpression were found in tumor xenografts. The expression of proteins were determined by the brown area. Scale bars = 50 μm. * p < 0.05, ** p < 0.01.

Article Snippet: The diluted primary antibodies against SRI (1:500, Proteintech, Wuhan, China), STAT3 (1:500, Proteintech, Wuhan, China), Bcl-XL (1:500, Proteintech, Wuhan, China), MCL-1 (1:500, Proteintech, Wuhan, China), p65 (1:500, Proteintech, Wuhan, China), p-p65 (1:500, Abmart, Shanghai, China), Bcl-2 (1:1000, Abcam, Cambridge, UK), Bax (1:1000, Proteintech, Wuhan, China), caspase3 (1:500, Proteintech, Wuhan, China), cIAP1 (1:500, Proteintech, Wuhan, China), p-IκB (1:500, Proteintech, Wuhan, China), tubulin (1:5000, Proteintech, Wuhan, China) and GAPDH (1:10000, Proteintech, Wuhan, China) were incubated with the membranes at 4 °C overnight.

Techniques: In Vitro, In Vivo, Fluorescence, Staining, Over Expression, Western Blot, Expressing

Journal: International Journal of Molecular Sciences

Article Title: Sorcin Inhibits Mitochondrial Apoptosis by Interacting with STAT3 via NF-κB Pathway

doi: 10.3390/ijms25137206

Figure Lengend Snippet: SRI and STAT3 interaction is crucial for anti-apoptosis. ( A – D ) Stattic reduced the fluorescence intensity of TMRE (shown in red) and enhanced the sensitivities to SRI induced apoptosis by Hoechst 33342 (shown in blue) staining assay. Scale bars = 200 μm. ( E – H ) The expression of SRI, p65, p-p65, p-IκB and apoptosis-related proteins were detected by Western blot in Stattic group. * p < 0.05, ** p < 0.01.

Article Snippet: The diluted primary antibodies against SRI (1:500, Proteintech, Wuhan, China), STAT3 (1:500, Proteintech, Wuhan, China), Bcl-XL (1:500, Proteintech, Wuhan, China), MCL-1 (1:500, Proteintech, Wuhan, China), p65 (1:500, Proteintech, Wuhan, China), p-p65 (1:500, Abmart, Shanghai, China), Bcl-2 (1:1000, Abcam, Cambridge, UK), Bax (1:1000, Proteintech, Wuhan, China), caspase3 (1:500, Proteintech, Wuhan, China), cIAP1 (1:500, Proteintech, Wuhan, China), p-IκB (1:500, Proteintech, Wuhan, China), tubulin (1:5000, Proteintech, Wuhan, China) and GAPDH (1:10000, Proteintech, Wuhan, China) were incubated with the membranes at 4 °C overnight.

Techniques: Fluorescence, Staining, Expressing, Western Blot

Journal: International Journal of Molecular Sciences

Article Title: Sorcin Inhibits Mitochondrial Apoptosis by Interacting with STAT3 via NF-κB Pathway

doi: 10.3390/ijms25137206

Figure Lengend Snippet: SRI inhibits cells apoptosis through the NF-κB signaling pathway. ( A , B ) Tumor volume and weight of orthotopic xenograft models derived from upSRI cells treated with Stattic. Symbols of different colors represented the weight of tumors in different groups. ( C , D ) Representative IHC images of STAT3, p65, p-p65, p-IκB and apoptosis-related proteins in Stattic treatment. Scale bars = 50 μm. ( C , E ) Representative IHC images of p65 and apoptosis-related proteins after treatment with AL inhibitor. The expression of proteins were determined by the brown area. ( F – H ) AL inhibits the fluorescence intensity of TMRE (shown in red) and reduced the sensitivities to SRI-induced apoptosis by Hoechst 33342 (shown in blue) staining assay. Scale bars = 200 μm. ( I – L ) Apoptosis-related proteins were detected after treated with AL inhibitor by Western blot. * p < 0.05, ** p < 0.01.

Article Snippet: The diluted primary antibodies against SRI (1:500, Proteintech, Wuhan, China), STAT3 (1:500, Proteintech, Wuhan, China), Bcl-XL (1:500, Proteintech, Wuhan, China), MCL-1 (1:500, Proteintech, Wuhan, China), p65 (1:500, Proteintech, Wuhan, China), p-p65 (1:500, Abmart, Shanghai, China), Bcl-2 (1:1000, Abcam, Cambridge, UK), Bax (1:1000, Proteintech, Wuhan, China), caspase3 (1:500, Proteintech, Wuhan, China), cIAP1 (1:500, Proteintech, Wuhan, China), p-IκB (1:500, Proteintech, Wuhan, China), tubulin (1:5000, Proteintech, Wuhan, China) and GAPDH (1:10000, Proteintech, Wuhan, China) were incubated with the membranes at 4 °C overnight.

Techniques: Derivative Assay, Expressing, Fluorescence, Staining, Western Blot

Journal: International Journal of Molecular Sciences

Article Title: Sorcin Inhibits Mitochondrial Apoptosis by Interacting with STAT3 via NF-κB Pathway

doi: 10.3390/ijms25137206

Figure Lengend Snippet: A schematic illustration of SRI regulating apoptosis in HCC. SRI overexpression inhibits the apoptosis of HCC cells by interacting with STAT3 via NF-κB pathway, whereas opposing effects were observed for knockdown of SRI. Stattic, an inhibitor of STAT3, promotes apoptosis of HCC cells via NF-κB pathway. Avicularin, the inhibitor of NF-κB, which promotes apoptosis of HCC cells. ↑ : represent upregulation or promotion. ↓ : represent downregulation or inhibition.

Article Snippet: The diluted primary antibodies against SRI (1:500, Proteintech, Wuhan, China), STAT3 (1:500, Proteintech, Wuhan, China), Bcl-XL (1:500, Proteintech, Wuhan, China), MCL-1 (1:500, Proteintech, Wuhan, China), p65 (1:500, Proteintech, Wuhan, China), p-p65 (1:500, Abmart, Shanghai, China), Bcl-2 (1:1000, Abcam, Cambridge, UK), Bax (1:1000, Proteintech, Wuhan, China), caspase3 (1:500, Proteintech, Wuhan, China), cIAP1 (1:500, Proteintech, Wuhan, China), p-IκB (1:500, Proteintech, Wuhan, China), tubulin (1:5000, Proteintech, Wuhan, China) and GAPDH (1:10000, Proteintech, Wuhan, China) were incubated with the membranes at 4 °C overnight.

Techniques: Over Expression, Knockdown, Inhibition

Journal: Nature Communications

Article Title: Translation velocity determines the efficacy of engineered suppressor tRNAs on pathogenic nonsense mutations

doi: 10.1038/s41467-024-47258-9

Figure Lengend Snippet: a Schematic of the readthrough experiments with plasmid-encoded PTC-FLuc constructs with a longer sequence upstream of the PTC (orange). IVT in vitro transcribed sup-tRNA, FLuc (green) firefly luciferase, CMV cytomegalovirus promoter, Met methionine start codon of FLuc , PTC (red) premature termination codon. Figure was generated using BioRender. b Computed translation velocity profiles of eW1282X PTC with extended sequence upstream of the PTC (blue) and codon- substituted variant smW1282X PTC (gray) of CFTR whose translation profile was smoothed to be within 1.5 σ . Inset, codon, and amino acid sequence of the segment with speed inversion (light green, substitution for a fast-translated codon; orange, substitution for a slow-translated codon). Horizontal dotted lines designate the ±1.5 σ interval. c Sup-tRNA mediated readthrough efficacy in CFBE41o - cells, with and without eRF1 inhibitor (SRI-41315, 5 µM) and normalized to wildtype FLuc extended with the same sequence without the PTC (eFLuc). Mis, mismatched tRNA that does not pair to the PTC (purple). Data are means ± s.e.m. ( n , independent replicates, e.g., n = 3 for eFLuc, mismatched tRNA, eW1282X and smW1282X with sup-tRNA and eRF1 inhibitor; n = 6 for eW1282X and smW1282X with sup-tRNA). Statistics, two-sided t test. Source data are provided as a file.

Article Snippet: In some experiments, cells were treated with 5 µM eRF1 inhibitor (SRI-41315, MedChemExpress) and after 18 h transfected with sup-tRNA as described above for 6 h (total duration of eRF1 treatment was 24 h).

Techniques: Plasmid Preparation, Construct, Sequencing, In Vitro, Luciferase, Generated, Variant Assay