Journal: Antimicrobial Agents and Chemotherapy

Article Title: Potentiation of Antibiotic Activity by a Novel Cationic Peptide: Potency and Spectrum of Activity of SPR741

doi: 10.1128/AAC.00200-17

Figure Lengend Snippet: Structure of SPR741.

Article Snippet: In line with previously reported results ( 12 ), in combination with SPR741, the MIC of rifampin was substantially reduced; in the present work, the MIC was reduced >8,000-fold to 0.002 μg/ml in the presence of 8 μg/ml SPR741 ( and ), substantially below the Clinical and Laboratory Standards Institute (CLSI) breakpoint for susceptibility of 1 μg/ml for Staphylococcus spp. ( 13 ).

Techniques:

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Potentiation of Antibiotic Activity by a Novel Cationic Peptide: Potency and Spectrum of Activity of SPR741

doi: 10.1128/AAC.00200-17

Figure Lengend Snippet: MICs of rifampin, clarithromycin, and azithromycin, each in the presence and absence of SPR741, against E. coli ATCC 25922, K. pneumoniae ATCC 43816, and A. baumannii NCTC 12156

Article Snippet: In line with previously reported results ( 12 ), in combination with SPR741, the MIC of rifampin was substantially reduced; in the present work, the MIC was reduced >8,000-fold to 0.002 μg/ml in the presence of 8 μg/ml SPR741 ( and ), substantially below the Clinical and Laboratory Standards Institute (CLSI) breakpoint for susceptibility of 1 μg/ml for Staphylococcus spp. ( 13 ).

Techniques:

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Potentiation of Antibiotic Activity by a Novel Cationic Peptide: Potency and Spectrum of Activity of SPR741

doi: 10.1128/AAC.00200-17

Figure Lengend Snippet: SPR741 potentiates the activities of antibiotics against E. coli ATCC 25922 (A), K. pneumoniae ATCC 43816 (B), and A. baumannii NCTC 12156 (C). The fold reduction in the MIC of each of the 35 antibiotics (grouped by class between broken lines) is displayed at each of three concentrations of SPR741: 2 μg/ml (white bars), 4 μg/ml (gray bars), and 8 μg/ml (black bars) (A) or 4 μg/ml (white bars), 8 μg/ml (gray bars), and 16 μg/ml (black bars) (B and C).

Article Snippet: In line with previously reported results ( 12 ), in combination with SPR741, the MIC of rifampin was substantially reduced; in the present work, the MIC was reduced >8,000-fold to 0.002 μg/ml in the presence of 8 μg/ml SPR741 ( and ), substantially below the Clinical and Laboratory Standards Institute (CLSI) breakpoint for susceptibility of 1 μg/ml for Staphylococcus spp. ( 13 ).

Techniques:

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Potentiation of Antibiotic Activity by a Novel Cationic Peptide: Potency and Spectrum of Activity of SPR741

doi: 10.1128/AAC.00200-17

Figure Lengend Snippet: Susceptibilities of E. coli strains to antibiotics that are substrates of the AcrAB-TolC efflux pump

Article Snippet: In line with previously reported results ( 12 ), in combination with SPR741, the MIC of rifampin was substantially reduced; in the present work, the MIC was reduced >8,000-fold to 0.002 μg/ml in the presence of 8 μg/ml SPR741 ( and ), substantially below the Clinical and Laboratory Standards Institute (CLSI) breakpoint for susceptibility of 1 μg/ml for Staphylococcus spp. ( 13 ).

Techniques:

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Potentiation of Antibiotic Activity by a Novel Cationic Peptide: Potency and Spectrum of Activity of SPR741

doi: 10.1128/AAC.00200-17

Figure Lengend Snippet: MIC 90 s of rifampin, mupirocin, azithromycin, fusidic acid, meropenem, aztreonam, retapamulin, and clarithromycin in the presence or absence of SPR741 against clinical and MDR isolate panels

Article Snippet: In line with previously reported results ( 12 ), in combination with SPR741, the MIC of rifampin was substantially reduced; in the present work, the MIC was reduced >8,000-fold to 0.002 μg/ml in the presence of 8 μg/ml SPR741 ( and ), substantially below the Clinical and Laboratory Standards Institute (CLSI) breakpoint for susceptibility of 1 μg/ml for Staphylococcus spp. ( 13 ).

Techniques:

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Assessment of the In Vivo Activity of SPR741 in Combination with Azithromycin against Multidrug-Resistant Enterobacteriaceae Isolates in the Neutropenic Murine Thigh Infection Model

doi: 10.1128/AAC.00239-18

Figure Lengend Snippet: MICs of AZM, SPR741, and the AZM-SPR741 combination a against Enterobacteriaceae isolates utilized in the in vivo activity studies

Article Snippet: The plasma samples were shipped to AIT Bioscience for determination of AZM and SPR741 concentrations.

Techniques: In Vivo, Activity Assay

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Assessment of the In Vivo Activity of SPR741 in Combination with Azithromycin against Multidrug-Resistant Enterobacteriaceae Isolates in the Neutropenic Murine Thigh Infection Model

doi: 10.1128/AAC.00239-18

Figure Lengend Snippet: Comparison of ƒAUC 0–24 values achieved with AZM in humans receiving 500 mg q24h i.v. and those in mice receiving a human-simulated regimen s.c.

Article Snippet: The plasma samples were shipped to AIT Bioscience for determination of AZM and SPR741 concentrations.

Techniques: Comparison

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Assessment of the In Vivo Activity of SPR741 in Combination with Azithromycin against Multidrug-Resistant Enterobacteriaceae Isolates in the Neutropenic Murine Thigh Infection Model

doi: 10.1128/AAC.00239-18

Figure Lengend Snippet: Concentration-time profile of free SPR741 in plasma in the neutropenic thigh infection model in mice receiving two different human-simulated AZM regimens. Data are means ± standard deviations.

Article Snippet: The plasma samples were shipped to AIT Bioscience for determination of AZM and SPR741 concentrations.

Techniques: Concentration Assay, Clinical Proteomics, Infection

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Assessment of the In Vivo Activity of SPR741 in Combination with Azithromycin against Multidrug-Resistant Enterobacteriaceae Isolates in the Neutropenic Murine Thigh Infection Model

doi: 10.1128/AAC.00239-18

Figure Lengend Snippet: Average change in log10 CFU ± SD at 24 h from the starting inoculum in a neutropenic murine thigh model with human-simulated AZM-SPR741. Isolates are ordered by increasing MICs of AZM alone. ***, AZM HSR (14 mg/kg) in combination with the SPR741 HSR of 5 doses (0 h, 36 mg/kg; 1.5 h, 33 mg/kg; 3.25 h, 23 mg/kg; 5 h, 16 mg/kg; 6.5 h, 12 mg/kg) administered every 8 h over 24 h.

Article Snippet: The plasma samples were shipped to AIT Bioscience for determination of AZM and SPR741 concentrations.

Techniques: