Journal: bioRxiv

Article Title: Coping with multiple enemies: pairwise interactions do not predict evolutionary change in complex multitrophic communities

doi: 10.1101/492132

Figure Lengend Snippet: Abundance (log-transformed, mean ± std. dev., n = 7) of the focal species B. subtilis (orange circles), competitor bacterium S. marcescens (red circles), predator P. caudatum (green circles) and B. subtilis-specific parasite phage SPP1 (purple circles) in each treatment over the duration of the experiment (Days 1-10).

Article Snippet: Our experiment consisted of seven sets of species combinations of Bacillus subtilis (NCIB3610) with (1) SPP1 phage parasite in isolation; (2) Paramecium caudatum , a generalist [ ; ; ] bacterial predator in isolation; (3) Serratia marcescens (ATCC 29632), a competitor of B. subtilis , in isolation; (4) P. caudatum and SPP1 phage; (5) S. marcescens and SPP1 phage; (6) S. marcescens and P. caudatum; and (7) S. marcescens, P. caudatum and SPP1 phage, resulting in a total of 7 experimental treatments, each replicated seven times.

Techniques: Transformation Assay

Journal: bioRxiv

Article Title: Coping with multiple enemies: pairwise interactions do not predict evolutionary change in complex multitrophic communities

doi: 10.1101/492132

Figure Lengend Snippet: The effect of community composition on B. subtilis evolution relative to the ancestral strain (solid line). a . Competitive ability of B. subtilis isolates, measured as the ratio of B. subtilis to S. marcescens (dashed line indicates a 1:1 ratio, solid line represents ancestral resistance). b . Relative resistance to parasite in B. subtilis across treatment groups. Resistance to phage SPP1 was measured as the difference in growth (optical density) of B. subtilis in the presence or absence of the ancestral phage parasite. The solid line represents the resistance of ancestral B. subtilis and the resistance of our experimental treatment isolates relative to ancestral parasite resistance. c . Growth of B. subtilis isolates in the presence of the predator P. caudatum (measured as log 10 optical density of biofilm production). Solid line indicates resistance of the ancestral B. subtilis grown in the presence of P. caudatum ). In all cases, values to the right of the solid lines indicate higher relative resistance than the ancestral strain, values to the left of the solid line indicate lower resistance relative to ancestral strain of B. subtilis .

Article Snippet: Our experiment consisted of seven sets of species combinations of Bacillus subtilis (NCIB3610) with (1) SPP1 phage parasite in isolation; (2) Paramecium caudatum , a generalist [ ; ; ] bacterial predator in isolation; (3) Serratia marcescens (ATCC 29632), a competitor of B. subtilis , in isolation; (4) P. caudatum and SPP1 phage; (5) S. marcescens and SPP1 phage; (6) S. marcescens and P. caudatum; and (7) S. marcescens, P. caudatum and SPP1 phage, resulting in a total of 7 experimental treatments, each replicated seven times.

Techniques:

Journal: bioRxiv

Article Title: Porcine and human aortic valve endothelial and interstitial cell isolation and characterization

doi: 10.1101/2022.12.01.518669

Figure Lengend Snippet: (a) Workflow of porcine VIC isolation. (b) Brightfield and immunofluorescence images of pVICs displaying a positive staining for a-SMA and VIM and a negative signal for PECAM-1 and vWF. (c) Migration assays revealed a scratch closure to 87% after 8 hours. (d) Gene expression analysis of pVICs were treated with osteogenic medium (OM) or pro-calcifying medium (PCM) for 7 days to induce in vitro calcification. Calcific markers, such as alkaline phosphatase ( ALPL ), bone morphogenic protein 2 ( BMP2 ), osteocalcin ( BGLAP ) and osteopontin ( SPP1 ) were significantly upregulated in OM and PCM. (e) Alizarin Red staining showed pronounced calcific nodules in OM and PCM, compared to control medium (CTM). n=3 donors, *P < 0.05, **P < 0.01, ***P < 0.001, ns not-significant, (b, c) analyzed by Student t-test, 2 tail, unpaired, (d) analyzed by one-way ANOVA with Bonferroni’s correction, scale bar 200μm, (a) created with BioRender.com.

Article Snippet: The following porcine TaqManTM Primers were used: GAPDH (Ss03375629_u1), α-SMA (Ss04245588_m1), vWF (Ss04322692_m1), PECAM-1 (Ss04322692_m1), VIM (Ss04330801_gH), ALPL (Ss06879568_m1), BMP2 (Ss03373798_g1), BGLAP (Ss03373655_s1), SPP1 (Ss03391321_m1).

Techniques: Isolation, Immunofluorescence, Staining, Migration, Gene Expression, In Vitro, Control

Journal: bioRxiv

Article Title: Porcine and human aortic valve endothelial and interstitial cell isolation and characterization

doi: 10.1101/2022.12.01.518669

Figure Lengend Snippet: Valvular endothelial cells (VECs), valvular interstitial cells (VICs), human VECs (hVECs), human VICs (hVICs), porcine VECs (pVECs), porcine VICs (pVICs), von Willebrand factor (vWF), platelet adhesion molecule-1 (PECAM-1), alpha smooth muscle actin (α-SMA), vimentin (VIM), alkaline phosphatase (ALPL), osteocalcin (BGLAP), runt-related factor X 2 (RUNX2), bone morphogenic protein 2 (BMP2), osteopontin (SPP1).

Article Snippet: The following porcine TaqManTM Primers were used: GAPDH (Ss03375629_u1), α-SMA (Ss04245588_m1), vWF (Ss04322692_m1), PECAM-1 (Ss04322692_m1), VIM (Ss04330801_gH), ALPL (Ss06879568_m1), BMP2 (Ss03373798_g1), BGLAP (Ss03373655_s1), SPP1 (Ss03391321_m1).

Techniques: