Journal: PLoS ONE

Article Title: Characterization of CD56 + Dendritic-Like Cells: A Normal Counterpart of Blastic Plasmacytoid Dendritic Cell Neoplasm?

doi: 10.1371/journal.pone.0081722

Figure Lengend Snippet: qRT-PCR analyses were performed using cDNA from pDLCs, pDCs, and mDCs. Results were standardized using PBMC cDNA (n=5). * P < 0.05 compared to pDLC. (A) TLR expression analysis revealed that mDCs expressed TLR2 and TLR4, whereas pDCs expressed TLR7 and TLR9. (B) Cytokine production was analyzed after CpG or LPS stimulation. (C) Transcripts of E2-2, Id2, Irf8, PU.1, and SpiB were analyzed in pDLCs, pDCs, and mDCs.

Article Snippet: Taqman Gene Expression Assays (Applied Biosystems) were as follows: Toll-like receptor (TLR) 2 Hs01872448_s1, TLR4 Hs00152939_m1, TLR7 Hs01933259_s1, TLR9 Hs00370913_s1, E2-2 Hs00162613_m1, ID2 Hs04187239_m1, IRF8 Hs00175238_m1, PU.1 Hs00745162_s1, SPIB Hs00162150_m1, IFN-α Hs00855471_g1, IL12 Hs 01073447_m1, IL10 Hs00961622_m1, IL6 Hs00985639_m1, TNF-α Hs01113624_g1, and GAPDH Hs02758991_g1.

Techniques: Quantitative RT-PCR, Expressing

Journal: iScience

Article Title: Neonatal infection with Bordetella pertussis promotes autism-like phenotypes in mice

doi: 10.1016/j.isci.2024.111548

Figure Lengend Snippet:

Article Snippet: Spib (Mm03048233_m1) , Applied Biosystems , Cat#: 4331182.

Techniques: Virus, Recombinant, Purification, Blocking Assay, Control, Reverse Transcription, Staining, Enzyme-linked Immunosorbent Assay, Software

Journal: Journal of Virology

Article Title: A Functional MicroRNA-155 Ortholog Encoded by the Oncogenic Marek's Disease Virus

doi: 10.1128/jvi.01166-08

Figure Lengend Snippet: FIG. 4. Repression of endogenous PU.1 levels by miR-M4 and miR-155. (a) Repression of luciferase reporter constructs of the MRE- containing region of the 3UTR of the PU.1 transcript or seed mutant (PU.1-mu2) cloned in psiCHECK-2 at the end of the Renilla luciferase gene. The histogram shows the relative Renilla luciferase activity in DF-1 cells transfected with empty vector or miRNA expression con- structs as indicated. (b) Western blot analysis of PU.1 protein expres- sion in stably selected HD11 cells. After transfection with expression vectors of miR-M4, miR-155, viral miR cluster 1, or a miR mutant, HD11 cells were selected with appropriate antibiotics. The untrans- fected HD11 cells were included as controls. (Top) A Western blot assay was carried out with polyclonal anti-SPIB antibodies; (bottom); for the loading control, the same blot was reprobed with antitubulin (-Tubulin) antibody. (c) Relative signal intensities of the PU.1 West- ern blot band were quantified using ImageQuant and normalized against the corresponding signal from the antitubulin band. The signal from untransfected control HD11 cells was set as 1.

Article Snippet: Western blot analysis of PU.1 levels using rabbit anti-SPIB polyclonal antibody (Aviva Systems Biology) showed that miR-M4 expressed alone or as part of the cluster and miR-155 had a negative effect on PU.1 levels compared to what was seen for control HD11 cells or mutant constructs (Fig. 4b and c).

Techniques: Luciferase, Construct, Mutagenesis, Clone Assay, Activity Assay, Transfection, Plasmid Preparation, Expressing, Western Blot, Stable Transfection, Control