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Bio-Logic Science
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Specsoft Inc
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Verlag GmbH
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Verlag GmbH
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Verlag GmbH
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Verlag GmbH
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Image Search Results
Journal: Toxicological Sciences
Article Title: Linking Inflammation and Parkinson Disease: Hypochlorous Acid Generates Parkinsonian Poisons
doi: 10.1093/toxsci/kfw052
Figure Lengend Snippet: The reactions of HOCl and dopamine as observed by UV-Vis spectrometry. The possible individual reactions and products for the reaction of hypochlorous acid (HOCl) and dopamine are given in (A), where 1: indoline-5,6-diol; 2: 2,3-dihydro-1H-indole-5,6-dione; 3: 1-chloro-2,3-dihydro-1H-indole-5,6-dione; 4: 4-(2-(chloroamino)ethyl)benzene-1,2-diol; 5: 1-chloroindoline-5,6-diol; 6: 4-(2-aminoethyl)-5-chlorobenzene-1,2-diol; 7: 4-chloro-5-(2-(chloroamino)ethyl)benzene-1,2-diol; 8: 4-(2-aminoethyl)cyclohexa-3,5-diene-1,2-dione; and 9: 4-(2-(chloroamino)ethyl)cyclohexa-3,5-diene-1,2-dione. Spectra were recorded using a Hewlet-Packard 8453 diode array spectrophotometer. The reaction was initiated by the addition of HOCl (150 µM final concentration) to dopamine (25 µM final concentration) and data were collected every 0.5 s for 300 s. A three-dimensional depiction of the data from 200 to 350 nm is shown (B). The resulting data set was exported and analyzed using global analysis, singular value decomposition via Specfit software (Specsoft Inc.). Data were fitted to a sequential model in which the reactants formed 2 successive transients followed by a final unchanging endpoint specie (C).
Article Snippet: The resulting data set was then exported as a single data file and analyzed using global analysis, singular value decomposition via
Techniques: Spectrophotometry, Concentration Assay, Software