spam1 Search Results


gene exp spam1 rn01500727 m1  (Thermo Fisher)
87
Thermo Fisher gene exp spam1 rn01500727 m1
Gene Exp Spam1 Rn01500727 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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recombinant bovine spam1  (R&D Systems)
91
R&D Systems recombinant bovine spam1
Effects of the S3 flavonoid on the activities of different hyaluronidases. (A) Effects of 20 μM S3 (“+”) on HA digestion by BTH, recombinant Hyal1, recombinant <t>PH20</t> <t>(rPH20),</t> and Streptomyces hyaluronidase compared to enzymes treated with vehicle alone (“-“). The “HA” lane was incubated without S3 but with the vehicle from the hyaluronidases. (B) Effects of the S3 flavonoid on HA digestion by lysates from HEK-293 cells transfected with empty vector (“vector”) or cells transfected with Hyal1, Hyal2, or PH20 compared to HA digested by rPH20 or BTH or to undigested HA “HA”. (C) Effects of different concentrations of S3 or vehicle alone (DMSO - “D”) on HA digestion by HEK-293 cells either untransfected (“untrans”), transfected with vector alone, or transfected with an expression vector carrying the cDNA for Cemip. (D) Quantification of the inhibition of HA digestion by S3 (20 μM) in the presence of recombinant Hyal1, recombinant PH20, BTH, or Streptomyces hyaluronidase. *p<0.01. (E) Quantification of HA digestion in live cell cultures of HEK-293 cells treated with S3 (20 μM) following transfection with either Hyal1, Hyal2, PH20 or Cemip expression vectors. *p<0.005.
Recombinant Bovine Spam1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gene exp spam1 rn00588656 m1  (Thermo Fisher)
85
Thermo Fisher gene exp spam1 rn00588656 m1
Effects of the S3 flavonoid on the activities of different hyaluronidases. (A) Effects of 20 μM S3 (“+”) on HA digestion by BTH, recombinant Hyal1, recombinant <t>PH20</t> <t>(rPH20),</t> and Streptomyces hyaluronidase compared to enzymes treated with vehicle alone (“-“). The “HA” lane was incubated without S3 but with the vehicle from the hyaluronidases. (B) Effects of the S3 flavonoid on HA digestion by lysates from HEK-293 cells transfected with empty vector (“vector”) or cells transfected with Hyal1, Hyal2, or PH20 compared to HA digested by rPH20 or BTH or to undigested HA “HA”. (C) Effects of different concentrations of S3 or vehicle alone (DMSO - “D”) on HA digestion by HEK-293 cells either untransfected (“untrans”), transfected with vector alone, or transfected with an expression vector carrying the cDNA for Cemip. (D) Quantification of the inhibition of HA digestion by S3 (20 μM) in the presence of recombinant Hyal1, recombinant PH20, BTH, or Streptomyces hyaluronidase. *p<0.01. (E) Quantification of HA digestion in live cell cultures of HEK-293 cells treated with S3 (20 μM) following transfection with either Hyal1, Hyal2, PH20 or Cemip expression vectors. *p<0.005.
Gene Exp Spam1 Rn00588656 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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csb el022471hu  (Cusabio)
90
Cusabio csb el022471hu
Effects of the S3 flavonoid on the activities of different hyaluronidases. (A) Effects of 20 μM S3 (“+”) on HA digestion by BTH, recombinant Hyal1, recombinant <t>PH20</t> <t>(rPH20),</t> and Streptomyces hyaluronidase compared to enzymes treated with vehicle alone (“-“). The “HA” lane was incubated without S3 but with the vehicle from the hyaluronidases. (B) Effects of the S3 flavonoid on HA digestion by lysates from HEK-293 cells transfected with empty vector (“vector”) or cells transfected with Hyal1, Hyal2, or PH20 compared to HA digested by rPH20 or BTH or to undigested HA “HA”. (C) Effects of different concentrations of S3 or vehicle alone (DMSO - “D”) on HA digestion by HEK-293 cells either untransfected (“untrans”), transfected with vector alone, or transfected with an expression vector carrying the cDNA for Cemip. (D) Quantification of the inhibition of HA digestion by S3 (20 μM) in the presence of recombinant Hyal1, recombinant PH20, BTH, or Streptomyces hyaluronidase. *p<0.01. (E) Quantification of HA digestion in live cell cultures of HEK-293 cells treated with S3 (20 μM) following transfection with either Hyal1, Hyal2, PH20 or Cemip expression vectors. *p<0.005.
Csb El022471hu, supplied by Cusabio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gene exp spam1 rn01500728 m1  (Thermo Fisher)
85
Thermo Fisher gene exp spam1 rn01500728 m1
Effects of the S3 flavonoid on the activities of different hyaluronidases. (A) Effects of 20 μM S3 (“+”) on HA digestion by BTH, recombinant Hyal1, recombinant <t>PH20</t> <t>(rPH20),</t> and Streptomyces hyaluronidase compared to enzymes treated with vehicle alone (“-“). The “HA” lane was incubated without S3 but with the vehicle from the hyaluronidases. (B) Effects of the S3 flavonoid on HA digestion by lysates from HEK-293 cells transfected with empty vector (“vector”) or cells transfected with Hyal1, Hyal2, or PH20 compared to HA digested by rPH20 or BTH or to undigested HA “HA”. (C) Effects of different concentrations of S3 or vehicle alone (DMSO - “D”) on HA digestion by HEK-293 cells either untransfected (“untrans”), transfected with vector alone, or transfected with an expression vector carrying the cDNA for Cemip. (D) Quantification of the inhibition of HA digestion by S3 (20 μM) in the presence of recombinant Hyal1, recombinant PH20, BTH, or Streptomyces hyaluronidase. *p<0.01. (E) Quantification of HA digestion in live cell cultures of HEK-293 cells treated with S3 (20 μM) following transfection with either Hyal1, Hyal2, PH20 or Cemip expression vectors. *p<0.005.
Gene Exp Spam1 Rn01500728 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gene exp spam1 hs00162139 m1  (Thermo Fisher)
85
Thermo Fisher gene exp spam1 hs00162139 m1
Effects of the S3 flavonoid on the activities of different hyaluronidases. (A) Effects of 20 μM S3 (“+”) on HA digestion by BTH, recombinant Hyal1, recombinant <t>PH20</t> <t>(rPH20),</t> and Streptomyces hyaluronidase compared to enzymes treated with vehicle alone (“-“). The “HA” lane was incubated without S3 but with the vehicle from the hyaluronidases. (B) Effects of the S3 flavonoid on HA digestion by lysates from HEK-293 cells transfected with empty vector (“vector”) or cells transfected with Hyal1, Hyal2, or PH20 compared to HA digested by rPH20 or BTH or to undigested HA “HA”. (C) Effects of different concentrations of S3 or vehicle alone (DMSO - “D”) on HA digestion by HEK-293 cells either untransfected (“untrans”), transfected with vector alone, or transfected with an expression vector carrying the cDNA for Cemip. (D) Quantification of the inhibition of HA digestion by S3 (20 μM) in the presence of recombinant Hyal1, recombinant PH20, BTH, or Streptomyces hyaluronidase. *p<0.01. (E) Quantification of HA digestion in live cell cultures of HEK-293 cells treated with S3 (20 μM) following transfection with either Hyal1, Hyal2, PH20 or Cemip expression vectors. *p<0.005.
Gene Exp Spam1 Hs00162139 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 85 stars, based on 1 article reviews
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elisa  (Cusabio)
90
Cusabio elisa
Effects of the S3 flavonoid on the activities of different hyaluronidases. (A) Effects of 20 μM S3 (“+”) on HA digestion by BTH, recombinant Hyal1, recombinant <t>PH20</t> <t>(rPH20),</t> and Streptomyces hyaluronidase compared to enzymes treated with vehicle alone (“-“). The “HA” lane was incubated without S3 but with the vehicle from the hyaluronidases. (B) Effects of the S3 flavonoid on HA digestion by lysates from HEK-293 cells transfected with empty vector (“vector”) or cells transfected with Hyal1, Hyal2, or PH20 compared to HA digested by rPH20 or BTH or to undigested HA “HA”. (C) Effects of different concentrations of S3 or vehicle alone (DMSO - “D”) on HA digestion by HEK-293 cells either untransfected (“untrans”), transfected with vector alone, or transfected with an expression vector carrying the cDNA for Cemip. (D) Quantification of the inhibition of HA digestion by S3 (20 μM) in the presence of recombinant Hyal1, recombinant PH20, BTH, or Streptomyces hyaluronidase. *p<0.01. (E) Quantification of HA digestion in live cell cultures of HEK-293 cells treated with S3 (20 μM) following transfection with either Hyal1, Hyal2, PH20 or Cemip expression vectors. *p<0.005.
Elisa, supplied by Cusabio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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elisa - by Bioz Stars, 2026-03
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gene exp spam1 hs01095939 m1  (Thermo Fisher)
85
Thermo Fisher gene exp spam1 hs01095939 m1
GusB, Hyal1 and klotho gene expression in arthritis patients' synovial membrane and fibroblast samples. Gene expression for β- D -glucuronidase (GusB), hyaluronidase 1 (Hyal1), and klotho genes was lower in rheumatoid arthritis (RA) and osteoarthritis (OA) synovial fibroblasts (SFs) than in RA and OA synovial membrane (SM) tissue samples (Mann-Whitney rank sum test). The Hyal1 gene expression was significantly higher in OA SM as compared with RA SM (Mann – Whitney rank sum test). The sperm adhesion molecule 1 gene expression was undetectable. Gex, gene expression; HGPRT, hypoxanthine phosphoribosyl transferase. * P < 0.05; ** P < 0.01; *** P < 0.001.
Gene Exp Spam1 Hs01095939 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 85 stars, based on 1 article reviews
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gene exp spam1 mm00486329 m1  (Thermo Fisher)
86
Thermo Fisher gene exp spam1 mm00486329 m1
GusB, Hyal1 and klotho gene expression in arthritis patients' synovial membrane and fibroblast samples. Gene expression for β- D -glucuronidase (GusB), hyaluronidase 1 (Hyal1), and klotho genes was lower in rheumatoid arthritis (RA) and osteoarthritis (OA) synovial fibroblasts (SFs) than in RA and OA synovial membrane (SM) tissue samples (Mann-Whitney rank sum test). The Hyal1 gene expression was significantly higher in OA SM as compared with RA SM (Mann – Whitney rank sum test). The sperm adhesion molecule 1 gene expression was undetectable. Gex, gene expression; HGPRT, hypoxanthine phosphoribosyl transferase. * P < 0.05; ** P < 0.01; *** P < 0.001.
Gene Exp Spam1 Mm00486329 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-spam1  (Abnova)
90
Abnova anti-spam1
GusB, Hyal1 and klotho gene expression in arthritis patients' synovial membrane and fibroblast samples. Gene expression for β- D -glucuronidase (GusB), hyaluronidase 1 (Hyal1), and klotho genes was lower in rheumatoid arthritis (RA) and osteoarthritis (OA) synovial fibroblasts (SFs) than in RA and OA synovial membrane (SM) tissue samples (Mann-Whitney rank sum test). The Hyal1 gene expression was significantly higher in OA SM as compared with RA SM (Mann – Whitney rank sum test). The sperm adhesion molecule 1 gene expression was undetectable. Gex, gene expression; HGPRT, hypoxanthine phosphoribosyl transferase. * P < 0.05; ** P < 0.01; *** P < 0.001.
Anti Spam1, supplied by Abnova, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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peptide spam1  (GL Biochem)
90
GL Biochem peptide spam1
GusB, Hyal1 and klotho gene expression in arthritis patients' synovial membrane and fibroblast samples. Gene expression for β- D -glucuronidase (GusB), hyaluronidase 1 (Hyal1), and klotho genes was lower in rheumatoid arthritis (RA) and osteoarthritis (OA) synovial fibroblasts (SFs) than in RA and OA synovial membrane (SM) tissue samples (Mann-Whitney rank sum test). The Hyal1 gene expression was significantly higher in OA SM as compared with RA SM (Mann – Whitney rank sum test). The sperm adhesion molecule 1 gene expression was undetectable. Gex, gene expression; HGPRT, hypoxanthine phosphoribosyl transferase. * P < 0.05; ** P < 0.01; *** P < 0.001.
Peptide Spam1, supplied by GL Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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spam1 gene  (Bioscientifica Ltd)
90
Bioscientifica Ltd spam1 gene
GusB, Hyal1 and klotho gene expression in arthritis patients' synovial membrane and fibroblast samples. Gene expression for β- D -glucuronidase (GusB), hyaluronidase 1 (Hyal1), and klotho genes was lower in rheumatoid arthritis (RA) and osteoarthritis (OA) synovial fibroblasts (SFs) than in RA and OA synovial membrane (SM) tissue samples (Mann-Whitney rank sum test). The Hyal1 gene expression was significantly higher in OA SM as compared with RA SM (Mann – Whitney rank sum test). The sperm adhesion molecule 1 gene expression was undetectable. Gex, gene expression; HGPRT, hypoxanthine phosphoribosyl transferase. * P < 0.05; ** P < 0.01; *** P < 0.001.
Spam1 Gene, supplied by Bioscientifica Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Effects of the S3 flavonoid on the activities of different hyaluronidases. (A) Effects of 20 μM S3 (“+”) on HA digestion by BTH, recombinant Hyal1, recombinant PH20 (rPH20), and Streptomyces hyaluronidase compared to enzymes treated with vehicle alone (“-“). The “HA” lane was incubated without S3 but with the vehicle from the hyaluronidases. (B) Effects of the S3 flavonoid on HA digestion by lysates from HEK-293 cells transfected with empty vector (“vector”) or cells transfected with Hyal1, Hyal2, or PH20 compared to HA digested by rPH20 or BTH or to undigested HA “HA”. (C) Effects of different concentrations of S3 or vehicle alone (DMSO - “D”) on HA digestion by HEK-293 cells either untransfected (“untrans”), transfected with vector alone, or transfected with an expression vector carrying the cDNA for Cemip. (D) Quantification of the inhibition of HA digestion by S3 (20 μM) in the presence of recombinant Hyal1, recombinant PH20, BTH, or Streptomyces hyaluronidase. *p<0.01. (E) Quantification of HA digestion in live cell cultures of HEK-293 cells treated with S3 (20 μM) following transfection with either Hyal1, Hyal2, PH20 or Cemip expression vectors. *p<0.005.

Journal: Glia

Article Title: A Modified Flavonoid Accelerates Oligodendrocyte Maturation and Functional Remyelination

doi: 10.1002/glia.23715

Figure Lengend Snippet: Effects of the S3 flavonoid on the activities of different hyaluronidases. (A) Effects of 20 μM S3 (“+”) on HA digestion by BTH, recombinant Hyal1, recombinant PH20 (rPH20), and Streptomyces hyaluronidase compared to enzymes treated with vehicle alone (“-“). The “HA” lane was incubated without S3 but with the vehicle from the hyaluronidases. (B) Effects of the S3 flavonoid on HA digestion by lysates from HEK-293 cells transfected with empty vector (“vector”) or cells transfected with Hyal1, Hyal2, or PH20 compared to HA digested by rPH20 or BTH or to undigested HA “HA”. (C) Effects of different concentrations of S3 or vehicle alone (DMSO - “D”) on HA digestion by HEK-293 cells either untransfected (“untrans”), transfected with vector alone, or transfected with an expression vector carrying the cDNA for Cemip. (D) Quantification of the inhibition of HA digestion by S3 (20 μM) in the presence of recombinant Hyal1, recombinant PH20, BTH, or Streptomyces hyaluronidase. *p<0.01. (E) Quantification of HA digestion in live cell cultures of HEK-293 cells treated with S3 (20 μM) following transfection with either Hyal1, Hyal2, PH20 or Cemip expression vectors. *p<0.005.

Article Snippet: Recombinant bovine SPAM1 (rPH20, 1.5 μg/ml, R&D Systems), Recombinant Human Hyaluronidase HYAL1 (rhHyal1, 6 μg/ml, R&D Systems), recombinant Human protein HYAL2 (rhHyal2, 40 μg/ml, MybioSource), Streptomyces hyaluronidase (StrepH; 7.5U/ml, Sigma), and bovine testicular hyaluronidase (BTH; Sigma, 10–20U/ml) were diluted in pH 3.5 or 4.5 (sodium citrate buffer) or pH 7.0 (phosphate-buffered saline, PBS) sample buffer.

Techniques: Recombinant, Incubation, Transfection, Plasmid Preparation, Expressing, Inhibition

Effects of blocking hyaluronidase activity on HA-mediated inhibition of OPC maturation. (A) Mouse OPCs grown under conditions that favor maturation in the absence of HA. (B) OPCs grown as in A in the presence of HMW HA. (C) OPCs grown as in B in the presence of 2 μM S3. (D) OPCs grown as in C in the presence of 25 U rPH20. (E) OPCs grown as in B in the presence of 25 μM Vcpal. Red = MBP; green = PDGFRα. Scale bars = 50 μm. (F) Quantification of the results in A-E. c = vehicle control; vc = Vcpal; P = PH20. Note that the data to the right of the dashed lines was from a separate experiment where HA was not added to the cultures. *p<0.001; **p<0.0005

Journal: Glia

Article Title: A Modified Flavonoid Accelerates Oligodendrocyte Maturation and Functional Remyelination

doi: 10.1002/glia.23715

Figure Lengend Snippet: Effects of blocking hyaluronidase activity on HA-mediated inhibition of OPC maturation. (A) Mouse OPCs grown under conditions that favor maturation in the absence of HA. (B) OPCs grown as in A in the presence of HMW HA. (C) OPCs grown as in B in the presence of 2 μM S3. (D) OPCs grown as in C in the presence of 25 U rPH20. (E) OPCs grown as in B in the presence of 25 μM Vcpal. Red = MBP; green = PDGFRα. Scale bars = 50 μm. (F) Quantification of the results in A-E. c = vehicle control; vc = Vcpal; P = PH20. Note that the data to the right of the dashed lines was from a separate experiment where HA was not added to the cultures. *p<0.001; **p<0.0005

Article Snippet: Recombinant bovine SPAM1 (rPH20, 1.5 μg/ml, R&D Systems), Recombinant Human Hyaluronidase HYAL1 (rhHyal1, 6 μg/ml, R&D Systems), recombinant Human protein HYAL2 (rhHyal2, 40 μg/ml, MybioSource), Streptomyces hyaluronidase (StrepH; 7.5U/ml, Sigma), and bovine testicular hyaluronidase (BTH; Sigma, 10–20U/ml) were diluted in pH 3.5 or 4.5 (sodium citrate buffer) or pH 7.0 (phosphate-buffered saline, PBS) sample buffer.

Techniques: Blocking Assay, Activity Assay, Inhibition, Control

GusB, Hyal1 and klotho gene expression in arthritis patients' synovial membrane and fibroblast samples. Gene expression for β- D -glucuronidase (GusB), hyaluronidase 1 (Hyal1), and klotho genes was lower in rheumatoid arthritis (RA) and osteoarthritis (OA) synovial fibroblasts (SFs) than in RA and OA synovial membrane (SM) tissue samples (Mann-Whitney rank sum test). The Hyal1 gene expression was significantly higher in OA SM as compared with RA SM (Mann – Whitney rank sum test). The sperm adhesion molecule 1 gene expression was undetectable. Gex, gene expression; HGPRT, hypoxanthine phosphoribosyl transferase. * P < 0.05; ** P < 0.01; *** P < 0.001.

Journal: Arthritis Research & Therapy

Article Title: Gene expression and activity of cartilage degrading glycosidases in human rheumatoid arthritis and osteoarthritis synovial fibroblasts

doi: 10.1186/ar2697

Figure Lengend Snippet: GusB, Hyal1 and klotho gene expression in arthritis patients' synovial membrane and fibroblast samples. Gene expression for β- D -glucuronidase (GusB), hyaluronidase 1 (Hyal1), and klotho genes was lower in rheumatoid arthritis (RA) and osteoarthritis (OA) synovial fibroblasts (SFs) than in RA and OA synovial membrane (SM) tissue samples (Mann-Whitney rank sum test). The Hyal1 gene expression was significantly higher in OA SM as compared with RA SM (Mann – Whitney rank sum test). The sperm adhesion molecule 1 gene expression was undetectable. Gex, gene expression; HGPRT, hypoxanthine phosphoribosyl transferase. * P < 0.05; ** P < 0.01; *** P < 0.001.

Article Snippet: Relative quantification of hexosaminidase A subunit (HexA), hexosaminidase B subunit (HexB), GusB, Hyal1, Hc-gp 39, klotho, Spam1, MMP1 and MMP3 mRNAs (referred to mRNA of hypoxanthine phosphoribosyl transferase) was performed with TaqMan quantitative-PCR assays (Hs00166843_m1, Hs00166864_m1, Hs99999908_m1, Hs00537920_g1, Hs00609691_m1, Hs00183100_m1, Hs01095939_m1, Hs00899658:m1 and Hs00233962_m1 referred to Hs99999909_m1, respectively) on an ABI PRISM 7000 Sequence Detector (Applied Biosystems, Foster City, CA, USA) using standard protocols [ ].

Techniques: Gene Expression, Membrane, MANN-WHITNEY