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Image Search Results
Journal: Journal of Molecular Endocrinology
Article Title: Sorcin is involved during embryo implantation via activating VEGF/PI3K/Akt pathway in mice
doi: 10.1530/jme-17-0153
Figure Lengend Snippet: Figure 1: Expression of sorcin during the period of early pregnancy and during estrous cycle in mouse
Article Snippet: 128 129 2.4 In vivo sorcin knock-down 130 Pregnant female mice underwent mini laparotomy under anesthesia on D3 of pregnancy to 131 deliver 50 nM of
Techniques: Expressing
Journal: Journal of Molecular Endocrinology
Article Title: Sorcin is involved during embryo implantation via activating VEGF/PI3K/Akt pathway in mice
doi: 10.1530/jme-17-0153
Figure Lengend Snippet: Figure 2: Immunohistochemistry of sorcin expression in mouse uteri during early pregnancy. Sorcin expression was measured in endometrium i.e. glandular epithelium (GE), luminal epithelium (LE), and stroma (S) at different days of pregnancy (D1, D4, D5, D6 and D7). Graph in lower panel shows image score analysis. IS, implantation site; NP, non-pregnant; NC, Negative control. p values are ap<0.001 vs.
Article Snippet: 128 129 2.4 In vivo sorcin knock-down 130 Pregnant female mice underwent mini laparotomy under anesthesia on D3 of pregnancy to 131 deliver 50 nM of
Techniques: Immunohistochemistry, Expressing, Negative Control
Journal: Journal of Molecular Endocrinology
Article Title: Sorcin is involved during embryo implantation via activating VEGF/PI3K/Akt pathway in mice
doi: 10.1530/jme-17-0153
Figure Lengend Snippet: Figure 3: Effect of sorcin inhibition on the number of implantation sites. The right horn was injected with
Article Snippet: 128 129 2.4 In vivo sorcin knock-down 130 Pregnant female mice underwent mini laparotomy under anesthesia on D3 of pregnancy to 131 deliver 50 nM of
Techniques: Inhibition, Injection
Journal: Journal of Molecular Endocrinology
Article Title: Sorcin is involved during embryo implantation via activating VEGF/PI3K/Akt pathway in mice
doi: 10.1530/jme-17-0153
Figure Lengend Snippet: Figure 5: Role of ovarian hormones in regulation of sorcin expression. (A) Ovariectomized mice treated with
Article Snippet: 128 129 2.4 In vivo sorcin knock-down 130 Pregnant female mice underwent mini laparotomy under anesthesia on D3 of pregnancy to 131 deliver 50 nM of
Techniques: Expressing
Journal: Journal of Molecular Endocrinology
Article Title: Sorcin is involved during embryo implantation via activating VEGF/PI3K/Akt pathway in mice
doi: 10.1530/jme-17-0153
Figure Lengend Snippet: Figure 6: Effect of sorcin silencing on cytosolic free Ca+2 , VEGF level, VEGFR-2 expression in mouse
Article Snippet: 128 129 2.4 In vivo sorcin knock-down 130 Pregnant female mice underwent mini laparotomy under anesthesia on D3 of pregnancy to 131 deliver 50 nM of
Techniques: Expressing
Journal: Journal of Molecular Endocrinology
Article Title: Sorcin is involved during embryo implantation via activating VEGF/PI3K/Akt pathway in mice
doi: 10.1530/jme-17-0153
Figure Lengend Snippet: Figure 7: The proliferation, migration and invasion assay of HUVEC cells. (A) The proliferation of HUVECs was detected in various groups; HUVECs were cultured with supernatants (20% V/V) from mouse primary EECs transfected with sorcin siRNA or scrambled siRNA. In additional group, a blocking anti-VEGF antibody was added in the supernatant from scrambled siRNA treated EECs. Details have been given in “materials and
Article Snippet: 128 129 2.4 In vivo sorcin knock-down 130 Pregnant female mice underwent mini laparotomy under anesthesia on D3 of pregnancy to 131 deliver 50 nM of
Techniques: Migration, Invasion Assay, Cell Culture, Transfection, Blocking Assay
Journal: Journal of Molecular Endocrinology
Article Title: Sorcin is involved during embryo implantation via activating VEGF/PI3K/Akt pathway in mice
doi: 10.1530/jme-17-0153
Figure Lengend Snippet: Figure 8: Schematic hypothetical representation of the molecular mechanism of sorcin in endometrium
Article Snippet: 128 129 2.4 In vivo sorcin knock-down 130 Pregnant female mice underwent mini laparotomy under anesthesia on D3 of pregnancy to 131 deliver 50 nM of
Techniques:
Journal: Diabetes
Article Title: Glucose-Induced Nuclear Shuttling of ChREBP Is Mediated by Sorcin and Ca 2+ Ions in Pancreatic β-Cells
doi: 10.2337/db10-1329
Figure Lengend Snippet: Sorcin controls ChREBP subcellular location in primary mouse β-cells. A : Dissociated mouse islets were transduced for 48 h with null or sorcin-overexpressing adenoviruses in 11 mmol/L glucose RPMI 1640, and then incubated 1 h in 3 mmol/L glucose followed by 6 h in 17 mmol/L RPMI 1640, before immunochemistry and confocal imaging as described in . Scale bars = 5 μm. C : Dissociated mouse islets were transduced with control or sorcin-shRNA lentiviruses for 72 h in 11 mmol/L RPMI 1640 and then incubated for 6 h in 3 mmol/L RPMI 1640 before immunochemistry and confocal imaging as above. B and C : Quantification of endogenous ChREBP in the nucleus, for the experiments presented in A and C . The intensities of ChREBP staining were measured using Volocity 4.0 software, for both nuclear and cytoplasmic regions of interest, in each individual cell, and background intensities were subtracted. Nuclear ChREBP intensity is expressed as percentage of cytoplasmic intensity ( n = 3 experiments, with 24 to 29 cells per condition). (A high-quality digital representation of this figure is available in the online issue.)
Article Snippet: Adenovirus production was then performed as in Ref. .
Techniques: Incubation, Imaging, Transduction, Control, shRNA, Staining, Software