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Image Search Results


Journal: STAR Protocols

Article Title: Measuring trogocytosis between ovarian tumor and natural killer cells

doi: 10.1016/j.xpro.2022.101425

Figure Lengend Snippet:

Article Snippet: Create gates for sorting using the relevant software e.g., BD FACSDiva TM or Sony SH800. c. For singlets, gate on FSC-H vs FSC-A. d. For live cells (DAPI-negative), gate singlets on DAPI vs FSC-A. e. For CD45+ NK-92 cells, gate live cells on CD45-APC vs FSC-A. f. For CD9+ and CD9- NK-92 cells, gate CD45+ NK-92 cells on CD9-PE vs CD45-APC. g. Sort using the purity mode. h. Collect sorted CD9+ and CD9- NK-92 cells in ice-cold FBS. i.

Techniques: Recombinant, Labeling, Staining, Electron Microscopy, Blocking Assay, Antibody Labeling, Expressing, Isolation, Software, Cell Counting, Microscopy, Cytometry, Real-time Polymerase Chain Reaction, Flow Cytometry, Spectrophotometry