Journal: Scientific reports

Article Title: Spinal gastrin releasing peptide receptor expressing interneurons are controlled by local phasic and tonic inhibition.

doi: 10.1038/s41598-019-52642-3

Figure Lengend Snippet: Figure 5. Grpr-Cre neurons expressing GCaMP6 are activated by somatostatin through disinhibition. Example traces from representative samples are shown. (a) Grpr-Cre neurons expressing GCaMP6 in an intact spinal cord. Numbers 1 and 2 indicate two neurons showing somatostatin-induced activity. (b) Fluorescence change traces from the intact spinal cord neurons indicated in a. (c) Proportion of Grpr-Cre neurons in the non-spontaneously active population expressing GCaMP6 in intact spinal cord that showed somatostatin-induced activity. (d) 300 µM coronal slice with Grpr-Cre neurons expressing GCaMP6. Numbers 1–4 indicate four different neurons showing somatostatin-induced activity. (e) Fluorescence change traces from the neurons indicated by 1–4 in (d–f) Proportion of Grpr-Cre neurons in the non-spontaneously active population expressing GCaMP6 in coronal slices that showed somatostatin-induced activity. (g) Mean values for fluorescence change (ΔF/F%) in GCaMP6 intensity for the intact cord and coronal slices, imaged at control conditions and when somatostatin was bath applied. Scale bar corresponds to 40 µm. The data is presented as mean ± standard error.

Article Snippet: 1 2Scientific RepoRtS | (2019) 9:16573 | https://doi.org/10.1038/s41598-019-52642-3 Spontaneous calcium imaging activities from superficial dorsal horn Grpr-Cre AAV9-CAG-DIO-GCaMP6infected neurons, and from superficial Grpr-Cre AAV9-CAG-DIO-GCaMP6-infected neurons in the presence of bath applied somatostatin (1 μM, Tocris), were recorded through a blue LED excitation light and signals were acquired in a frame rate of 2 Hz.

Techniques: Expressing, Activity Assay, Fluorescence, Control

Journal: Cancers

Article Title: Differentially Expressed Somatostatin (SST) and Its Receptors (SST1-5) in Sporadic Colorectal Cancer and Normal Colorectal Mucosa

doi: 10.3390/cancers16213584

Figure Lengend Snippet: Comparison of the quantitative SST and SST1-5 mRNA expression in CRC patients and in histologically normal colorectal mucosa from the same patients (C).

Article Snippet: Rabbit polyclonal antibodies specific for SST1 (orb229340, at 1:500 dilution), SST2 (orb221903, at 1:500 dilution), SST3 (orb11423, at 1:50 dilution) (all Abs from Biorbyt Ltd., Cambridge, CB5 8LA, UK), SST4 (DF2779, at 1:200 dilution) (Affinity Biosciences (Middlesex, UK), www.affbiotech.com ), SST5 (orb11424, at 1:200 dilution) (Biorbyt Ltd.), and Ki-67 antigen (clone MIB-1) (Dako Denmark A/S, Glostrup, Denmark, ready to use) were used.

Techniques: Comparison, Expressing

Journal: Cancers

Article Title: Differentially Expressed Somatostatin (SST) and Its Receptors (SST1-5) in Sporadic Colorectal Cancer and Normal Colorectal Mucosa

doi: 10.3390/cancers16213584

Figure Lengend Snippet: Values of Spearman’s coefficient for correlation between reciprocal expression of SST and SST1-5 mRNA in colorectal cancer samples (CRC) and non-tumor samples (C).

Article Snippet: Rabbit polyclonal antibodies specific for SST1 (orb229340, at 1:500 dilution), SST2 (orb221903, at 1:500 dilution), SST3 (orb11423, at 1:50 dilution) (all Abs from Biorbyt Ltd., Cambridge, CB5 8LA, UK), SST4 (DF2779, at 1:200 dilution) (Affinity Biosciences (Middlesex, UK), www.affbiotech.com ), SST5 (orb11424, at 1:200 dilution) (Biorbyt Ltd.), and Ki-67 antigen (clone MIB-1) (Dako Denmark A/S, Glostrup, Denmark, ready to use) were used.

Techniques: Expressing

Journal: Cancers

Article Title: Differentially Expressed Somatostatin (SST) and Its Receptors (SST1-5) in Sporadic Colorectal Cancer and Normal Colorectal Mucosa

doi: 10.3390/cancers16213584

Figure Lengend Snippet: Incidence of somatostatin (SST) and SST receptor (SST1-5) expression in colorectal cancer (CRC), lymph node metastasis (LNM), and control colorectal mucosa (C).

Article Snippet: Rabbit polyclonal antibodies specific for SST1 (orb229340, at 1:500 dilution), SST2 (orb221903, at 1:500 dilution), SST3 (orb11423, at 1:50 dilution) (all Abs from Biorbyt Ltd., Cambridge, CB5 8LA, UK), SST4 (DF2779, at 1:200 dilution) (Affinity Biosciences (Middlesex, UK), www.affbiotech.com ), SST5 (orb11424, at 1:200 dilution) (Biorbyt Ltd.), and Ki-67 antigen (clone MIB-1) (Dako Denmark A/S, Glostrup, Denmark, ready to use) were used.

Techniques: Expressing, Control

Journal: Cancers

Article Title: Differentially Expressed Somatostatin (SST) and Its Receptors (SST1-5) in Sporadic Colorectal Cancer and Normal Colorectal Mucosa

doi: 10.3390/cancers16213584

Figure Lengend Snippet: Detection of IHC expression pattern of SST receptors (SST1-5) in colorectal cancer (CRC), lymph node metastasis (LNM), and control colorectal mucosa (C).

Article Snippet: Rabbit polyclonal antibodies specific for SST1 (orb229340, at 1:500 dilution), SST2 (orb221903, at 1:500 dilution), SST3 (orb11423, at 1:50 dilution) (all Abs from Biorbyt Ltd., Cambridge, CB5 8LA, UK), SST4 (DF2779, at 1:200 dilution) (Affinity Biosciences (Middlesex, UK), www.affbiotech.com ), SST5 (orb11424, at 1:200 dilution) (Biorbyt Ltd.), and Ki-67 antigen (clone MIB-1) (Dako Denmark A/S, Glostrup, Denmark, ready to use) were used.

Techniques: Expressing, Control

Journal: Cancers

Article Title: Differentially Expressed Somatostatin (SST) and Its Receptors (SST1-5) in Sporadic Colorectal Cancer and Normal Colorectal Mucosa

doi: 10.3390/cancers16213584

Figure Lengend Snippet: Comparison of the quantitative SST and SST1-5 immunoexpression in colorectal cancer (CRC), lymph node metastasis (LNM), and control mucosa (C) (A%).

Article Snippet: Rabbit polyclonal antibodies specific for SST1 (orb229340, at 1:500 dilution), SST2 (orb221903, at 1:500 dilution), SST3 (orb11423, at 1:50 dilution) (all Abs from Biorbyt Ltd., Cambridge, CB5 8LA, UK), SST4 (DF2779, at 1:200 dilution) (Affinity Biosciences (Middlesex, UK), www.affbiotech.com ), SST5 (orb11424, at 1:200 dilution) (Biorbyt Ltd.), and Ki-67 antigen (clone MIB-1) (Dako Denmark A/S, Glostrup, Denmark, ready to use) were used.

Techniques: Comparison, Control

Journal: Cancers

Article Title: Differentially Expressed Somatostatin (SST) and Its Receptors (SST1-5) in Sporadic Colorectal Cancer and Normal Colorectal Mucosa

doi: 10.3390/cancers16213584

Figure Lengend Snippet: Values of Spearman’s coefficient for correlation between reciprocal immunoexpression of SST and SST1-5 in colorectal cancer samples (CRC), lymph node metastasis (LNM), and control colorectal mucosa (C).

Article Snippet: Rabbit polyclonal antibodies specific for SST1 (orb229340, at 1:500 dilution), SST2 (orb221903, at 1:500 dilution), SST3 (orb11423, at 1:50 dilution) (all Abs from Biorbyt Ltd., Cambridge, CB5 8LA, UK), SST4 (DF2779, at 1:200 dilution) (Affinity Biosciences (Middlesex, UK), www.affbiotech.com ), SST5 (orb11424, at 1:200 dilution) (Biorbyt Ltd.), and Ki-67 antigen (clone MIB-1) (Dako Denmark A/S, Glostrup, Denmark, ready to use) were used.

Techniques: Control, Expressing

Journal: Cancers

Article Title: Differentially Expressed Somatostatin (SST) and Its Receptors (SST1-5) in Sporadic Colorectal Cancer and Normal Colorectal Mucosa

doi: 10.3390/cancers16213584

Figure Lengend Snippet: Comparison of SST1-5 expression in relation to different IHC patterns of cellular localization.

Article Snippet: Rabbit polyclonal antibodies specific for SST1 (orb229340, at 1:500 dilution), SST2 (orb221903, at 1:500 dilution), SST3 (orb11423, at 1:50 dilution) (all Abs from Biorbyt Ltd., Cambridge, CB5 8LA, UK), SST4 (DF2779, at 1:200 dilution) (Affinity Biosciences (Middlesex, UK), www.affbiotech.com ), SST5 (orb11424, at 1:200 dilution) (Biorbyt Ltd.), and Ki-67 antigen (clone MIB-1) (Dako Denmark A/S, Glostrup, Denmark, ready to use) were used.

Techniques: Comparison, Expressing

Journal: Cancers

Article Title: Differentially Expressed Somatostatin (SST) and Its Receptors (SST1-5) in Sporadic Colorectal Cancer and Normal Colorectal Mucosa

doi: 10.3390/cancers16213584

Figure Lengend Snippet: Values of Spearman’s coefficient for correlation between SST and or SST1-5 (mRNA and peptide) in colorectal cancer (CRC) and control mucosa samples (C).

Article Snippet: Rabbit polyclonal antibodies specific for SST1 (orb229340, at 1:500 dilution), SST2 (orb221903, at 1:500 dilution), SST3 (orb11423, at 1:50 dilution) (all Abs from Biorbyt Ltd., Cambridge, CB5 8LA, UK), SST4 (DF2779, at 1:200 dilution) (Affinity Biosciences (Middlesex, UK), www.affbiotech.com ), SST5 (orb11424, at 1:200 dilution) (Biorbyt Ltd.), and Ki-67 antigen (clone MIB-1) (Dako Denmark A/S, Glostrup, Denmark, ready to use) were used.

Techniques: Control, Expressing

Journal: Cancers

Article Title: Differentially Expressed Somatostatin (SST) and Its Receptors (SST1-5) in Sporadic Colorectal Cancer and Normal Colorectal Mucosa

doi: 10.3390/cancers16213584

Figure Lengend Snippet: Comparison of SST1-5 immunoexpression in colorectal cancer (CRC) and control samples (C) in relation to different anatomical sites of the large intestine.

Article Snippet: Rabbit polyclonal antibodies specific for SST1 (orb229340, at 1:500 dilution), SST2 (orb221903, at 1:500 dilution), SST3 (orb11423, at 1:50 dilution) (all Abs from Biorbyt Ltd., Cambridge, CB5 8LA, UK), SST4 (DF2779, at 1:200 dilution) (Affinity Biosciences (Middlesex, UK), www.affbiotech.com ), SST5 (orb11424, at 1:200 dilution) (Biorbyt Ltd.), and Ki-67 antigen (clone MIB-1) (Dako Denmark A/S, Glostrup, Denmark, ready to use) were used.

Techniques: Comparison, Control

Journal: Cancers

Article Title: Differentially Expressed Somatostatin (SST) and Its Receptors (SST1-5) in Sporadic Colorectal Cancer and Normal Colorectal Mucosa

doi: 10.3390/cancers16213584

Figure Lengend Snippet: Comparison of SST and SST1-5 mRNA expression in colorectal cancer (CRC) in relation to mucinous vs. nonmucinous subtypes of CRC.

Article Snippet: Rabbit polyclonal antibodies specific for SST1 (orb229340, at 1:500 dilution), SST2 (orb221903, at 1:500 dilution), SST3 (orb11423, at 1:50 dilution) (all Abs from Biorbyt Ltd., Cambridge, CB5 8LA, UK), SST4 (DF2779, at 1:200 dilution) (Affinity Biosciences (Middlesex, UK), www.affbiotech.com ), SST5 (orb11424, at 1:200 dilution) (Biorbyt Ltd.), and Ki-67 antigen (clone MIB-1) (Dako Denmark A/S, Glostrup, Denmark, ready to use) were used.

Techniques: Comparison, Expressing

Journal: Cancers

Article Title: Differentially Expressed Somatostatin (SST) and Its Receptors (SST1-5) in Sporadic Colorectal Cancer and Normal Colorectal Mucosa

doi: 10.3390/cancers16213584

Figure Lengend Snippet: Comparison of SST and SST1-5 immunoexpression in colorectal cancer (CRC) in relation to mucinous vs. nonmucinous CRC subtypes.

Article Snippet: Rabbit polyclonal antibodies specific for SST1 (orb229340, at 1:500 dilution), SST2 (orb221903, at 1:500 dilution), SST3 (orb11423, at 1:50 dilution) (all Abs from Biorbyt Ltd., Cambridge, CB5 8LA, UK), SST4 (DF2779, at 1:200 dilution) (Affinity Biosciences (Middlesex, UK), www.affbiotech.com ), SST5 (orb11424, at 1:200 dilution) (Biorbyt Ltd.), and Ki-67 antigen (clone MIB-1) (Dako Denmark A/S, Glostrup, Denmark, ready to use) were used.

Techniques: Comparison

Journal: Cancers

Article Title: Differentially Expressed Somatostatin (SST) and Its Receptors (SST1-5) in Sporadic Colorectal Cancer and Normal Colorectal Mucosa

doi: 10.3390/cancers16213584

Figure Lengend Snippet: Values of Spearman’s coefficient for correlation between SST and SST1-5 mRNA in colorectal cancer (CRC), control colorectal mucosa (C), age of the patients, and basic laboratory tests.

Article Snippet: Rabbit polyclonal antibodies specific for SST1 (orb229340, at 1:500 dilution), SST2 (orb221903, at 1:500 dilution), SST3 (orb11423, at 1:50 dilution) (all Abs from Biorbyt Ltd., Cambridge, CB5 8LA, UK), SST4 (DF2779, at 1:200 dilution) (Affinity Biosciences (Middlesex, UK), www.affbiotech.com ), SST5 (orb11424, at 1:200 dilution) (Biorbyt Ltd.), and Ki-67 antigen (clone MIB-1) (Dako Denmark A/S, Glostrup, Denmark, ready to use) were used.

Techniques: Control

Journal: European journal of immunology

Article Title: CD4 + T cells regulate glucose homeostasis independent of adipose tissue dysfunction in mice.

doi: 10.1002/eji.202048870

Figure Lengend Snippet: Figure 6. Impact of 14-day CD4 depletion on pancreatic tissue integrity and secretory function. (A) Hematoxylin and eosin staining of pancreatic tissue (representative images of islets, data analysis shown in B and C). (B and C) Morphometric analysis of pancreatic tissue as measured by number of islets per mm2 pancreatic tissue (B; n = 6 per group; cumulative data from three independent experiments) and islet diameter (C; n = 6 per group; cumulative data from 3 independent experiments). (D) Frequency distribution analysis of islet size (shown as histogram with 20 μm bin width (n = 6 per group; cumulative data from three independent experiments). (E) Fasting plasma insulin in mice treated with either CD4- depleting or control antibody was measured using ELISA (n = 8 per group; cumulative data from four independent experiments). (F) Representative Western blots of insulin, somatostatin, glucagon, GLUT2 and GAPDH following depletion of CD4-positive cells or injection of an appropriate isotype control. Uncropped Western blots can be found in Supporting Information Fig. 4. (G-I) The abundance of insulin (G; both groups n = 8), glucagon (H; both groups n = 8), and somatostatin (I; both groups n = 8) is normalized to both GAPDH to account for whole pancreatic cell mass and GLUT2 to account for islet cell mass (panel G, H, and I represent cumulative data from four independent experiments with four mice per experiment). Data are presented as means ± SEM and as column bar graphs and were tested for statistical significance by unpaired t-testing, *p < 0.05. **p < 0.01. Scale bar represents 50 μm.

Article Snippet: Equal amounts of protein (30 μg) were loaded to SDS-PAGE and transferred to nitrocellulose membrane After blocking with 1% ROTI®Block (Carl Roth, Karlsruhe, Germany) or 5% dry milk in TBS/T, blots were incubated with primary antibodies against insulin (1:1000, STJ24210; St John‘s Laboratory, London, United Kingdom), glucagon (1:500, PA5-13442; Thermo Fisher Scientific), somatostatin (1:2000, STJ95730; St John’s Laboratory), GLUT2 (1:4000, 07–1402, Merck Millipore, Burlington, USA), and GAPDH (1:1000, 3686, Cell Signaling Technology, Boston, USA).

Techniques: Staining, Clinical Proteomics, Control, Enzyme-linked Immunosorbent Assay, Western Blot, Injection