Journal: JCI Insight

Article Title: Mutations of CNTNAP1 led to defects in neuronal development

doi: 10.1172/jci.insight.135697

Figure Lengend Snippet: ( A ) Representative Golgi staining of cortical layers II/III of adult Cntnap1 −/− mice (apical dendrites, 5 months of age, n = 3 for WT and Cntnap1 −/− mice). Scale bar: 4 μm. ( B ) Costaining of Cntnap1 and PV in WT mouse brain at P15. Scale bar: 200 μm. ( C ) Representative immunochemical images of the S1 primary somatosensory cortex and hippocampus in coronal slices of P15 mouse brains stained with a PV antibody. Scale bar: 500 μm. ( D ) Quantitative analysis of spine density in Cntnap1 –/– mice (22 and 25 neurons from each group were randomly selected and measured). Statistical significance was evaluated by Student’s t test. ** P = 0.0093. Data are shown as mean ± SEM. ( E and F ) Quantitative analysis of the number of PV + neurons in Cntnap1 –/– mice ( n = 3 mice for WT and KO) compared with their normal littermates. Statistical significance was evaluated by Student’s t test. Data are shown as mean ± SEM. For the quantification of PV + neurons in cortex, *** P = 0.001. For the quantification of PV + neurons in hippocampus, ** P = 0.0025. ( G and H ) Representative immunochemical images of the S1 primary somatosensory cortex and hippocampus in coronal slices of P15 mouse brains stained with a VIP (green) and SST (red) antibody. Scale bar: 500 μm. On the right are enlarged images of boxed hippocampus stained with VIP antibody. ( I and J ) Quantitative analysis of the number of VIP + and SST + neurons in Cntnap1 –/– mice compared with WT ( n = 3 mice for WT and KO). For the quantification of VIP + neurons in hippocampus, *** P = 0.0002. Statistical significance was evaluated by Student’s t test. Data are shown as mean ± SEM.

Article Snippet: Then, the brains were immersed in Golgi staining buffer (1:1 solution A/B) 1 day before according to the guidelines of the FD Rapid GolgiStainTM Kit (FD NeuroTechnologies, PK401).

Techniques: Staining