Journal: Experimental parasitology

Article Title: Typification of virulent and low virulence Babesia bigemina clones by 18S rRNA and rap-1c.

doi: 10.1016/j.exppara.2014.03.016

Figure Lengend Snippet: Fig. 1. 18S rRNA PCR amplification. Scheme of the B. bigemina 18S rRNA gene and amplified sequences. Arrows indicate the orientations and numbers indicate the location of the oligonucleotides used according to JG-29 18S rRNA sequence. The 18S rRNA gene is represented by an open box, and the PCR-amplified fragments are indicated with patterned boxes.

Article Snippet: Finally, the DNA fragments were purified using a commercial kit (Qiagen ) and sequenced using dye terminator sequencing (Macrogen , USA).

Techniques: Sequencing

Journal: Experimental parasitology

Article Title: Typification of virulent and low virulence Babesia bigemina clones by 18S rRNA and rap-1c.

doi: 10.1016/j.exppara.2014.03.016

Figure Lengend Snippet: Fig. 3. rt-PCR. Melting temperatures obtained by high-resolution melting analysis for the 287 bp rap-1c sequence of B. bigemina reference clones Bbi2A1 and Bbi9P1 and the JG-29 strain. The dashed line corresponds to Gr2 (attenuated genotype), the dotted line corresponds to Gr3 (pathogenic genotype), and the solid line corre- sponds to Gr1 (JG29 genotype). The range of analyzed temperatures was 83–93 C. dF/dT is the first derivative from Fluorescence/Temperature, and 0.32 C and 0.37 C are the differences observed between the genotypes.

Article Snippet: Finally, the DNA fragments were purified using a commercial kit (Qiagen ) and sequenced using dye terminator sequencing (Macrogen , USA).

Techniques: Reverse Transcription Polymerase Chain Reaction, Sequencing, Clone Assay, Fluorescence