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Journal: Molecular cell

Article Title: In vivo evidence for ATPase-dependent DNA translocation by the Bacillus subtilis SMC condensin complex

doi: 10.1016/j.molcel.2018.07.006

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-SMC polyclonal rabbit antibody ( Lindow et al., 2002 ) N/A Anti-ParB polyclonal rabbit antibody ( Lin et al., 1997 ) N/A Anti-ScpA polyclonal rabbit antibody, affinity purified ( Wang et al., 2017 ) N/A Anti-ScpA polyclonal rabbit antibody, affinity purified ( Wang et al., 2017 ) N/A Anti-SigA polyclonal rabbit antibody ( Fujita, 2000 ) N/A Chemicals, Peptides, and Recombinant Proteins Benzonase EMD Cat # 7046 Ni-NTA agarose Qiagen Cat # 30230 HiTrap Q HP GE Healthcare Cat # 17115301 Bradford Assay Kit ThermoFisher Cat # 23236 ATP Dot Scientific Cat # DSA30030–5 NADH Acros Organic Cat # 271100010 Phosphoenolypyruvate Tokyo Chemical Industry Cat # P0256 Rabbit Pyruvate Kinase Roche Diagnostics Cat # 10109045001 Lactate Dehydrogenase Sigma, Darmstadt, Germany Cat # L2500–25KU Formaldehyde 37% Sigma Cat # F8775 Ready-Lyse Lysozyme Epicentre Cat # R1802M HindIII NEB Cat # R0104M Klenow NEB Cat # M0210L Biotin-14-dATP ThermoFisher Cat # 19524016 T4 DNA ligase NEB Cat # M0202M Proteinase K NEB Cat # P8107S T4 DNA Polymerase NEB Cat # M0203L Critical Commercial Assays ATPase/GTPase Activity Assay Kit Sigma Cat # MAK113 NEBNext Ultra II DNA Library Prep Kit NEB Cat # E7645S Deposited Data Raw and analyzed Hi-C data This paper GEO: {"type":"entrez-geo","attrs":{"text":"GSE95137","term_id":"95137"}} GSE95137 Original Data Mendeley Data http://dx.doi.org/10.17632/tygwp234gr.1 Experimental Models: Organisms/Strains Bacillus subtilis strains, see Table S1 Oligonucleotides See Table S3 Recombinant DNA See Table S2 Software and Algorithms MATLAB 8.5 (R2015) ( Wang et al., 2017 ) https://www.mathworks.com/ hiclib ( Imakaev et al., 2012 ) https://bitbucket.org/mirnylab/hiclib Open in a separate window KEY RESOURCES TABLE

Techniques: Affinity Purification, Recombinant, Bradford Assay, Activity Assay, Software